玉簪属植物花瓣中类黄酮化合物价值评价

草业学报 ›› 2013, Vol. 22 ›› Issue (1): 234-244.

玉簪属植物花瓣中类黄酮化合物价值评价

刘妮娜^1,2,李晓东^1*,张金政^1*,王亮生¹,孙国峰¹,林秦文¹,徐彦军³

1.中国科学院植物研究所,北京 100093;
2.中国科学院研究生院,北京 100049;
3.中国农业大学理学院,北京 100094

收稿日期:2012-01-18 出版日期:2013-01-25 发布日期:2013-02-20
通讯作者: E-mail:lixcds1234@ibcas.ac.cn;caohua@ibcas.ac.cn
作者简介:刘妮娜(1987-),女,山东潍坊人,在读硕士。E-mail:liunn007@163.com
基金资助:
中国科学院知识创新工程重要方向项目(KSCX2-EW-B-02,KSCX2-EW-B-05)资助。

Flavonoid compounds in the petals of Hosta

LIU Ni-na^1,2, LI Xiao-dong¹, ZHANG Jin-zheng¹, WANG Liang-sheng¹, SUN Guo-feng¹, LIN Qin-wen¹, XU Yan-jun³

1.Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China;
2.Graduate University of the Chinese Academy of Sciences, Beijing 100049, China;
3.Department of Applied Chemistry, China Agricultural University, Beijing 100094, China

Received:2012-01-18 Online:2013-01-25 Published:2013-02-20

摘要/Abstract

摘要： 为拓宽玉簪属植物除园林方面的应用,本研究以玉簪属5个野生种和85个栽培品种的花瓣为材料,利用高效液相色谱(HPLC-DAD)技术,建立了定性定量分析玉簪花中类黄酮化合物的HPLC方法,可作为玉簪花药材的质量控制方法。同时结合质谱(MSⁿ)技术,鉴定了玉簪花瓣中17种类黄酮化合物的化学结构,分别为:山奈酚 3,7-二葡萄糖苷、山奈酚 3-葡萄糖-7-芸香糖苷、异鼠李素 3,4-二葡萄糖苷、山奈酚 3-奎尼酰葡萄糖苷、山奈酚 3-芥子酰葡萄糖苷、山奈酚 3-抗坏血酰葡萄糖苷、山奈酚 7-新橙皮糖苷、山奈酚 3,7-二己糖苷、山奈酚 3-葡萄糖-7-鼠李糖苷、木犀草素 7-新橙皮糖苷、槲皮素3-葡萄糖苷、槲皮素 3-葡糖苷酸、槲皮素 3-刺槐二糖苷、山奈酚 3-葡萄糖苷、山奈酚 3-葡糖苷酸、山奈酚 3-戊糖苷和山奈酚 7-葡萄糖苷。并比较了90种玉簪花中类黄酮化合物成分与含量的差异性,筛选出类黄酮化合物含量较高的种类。

Abstract: The flavonoid composition of five Hosta species and 85 Hosta cultivars was investigated by HPLC-DAD for the first time to expand the applications of Hosta. Qualitative and quantitative analysis methods for total flavonoids in flowers of Hosta were used as a basis for the quality control of Hosta. A combination of powerful tools (HPLC-DAD and HPLC-MSⁿ), were used to characterize seventeen kinds of flavonoid including kaempferol 3,7-di-O-glucoside, kaempferol 3-O-glucosyl-7-O-rutinose, isorhamnetin 3,4-di-O-glucoside, kaempferol 3-O-quinoylglucoside, kaempferol 3-O-sinapoylglucoside, kaempferol 3-O- ascorbicoylglucoside, kaempferol 7-O-neohesperidoside, kaempferol 3,7-di-O-hexoside, kaempferol 3-O-glucosyl-7-O-rhamnoside, luteolin 7-O-neohesperidoside, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, kaempferol 3-O-robinobioside, kaempferol 3-O-glucoside, kaempferol 3-O-glucuronide, kaempferol 3-O-pentose, and kaempferol 7-O-glucoside. The flavonoid contents of 90 kinds of Hosta flowers were compared and the high-value species were selected.

中图分类号:

Q949.71⁺8.23

刘妮娜,李晓东,张金政,王亮生,孙国峰,林秦文,徐彦军. 玉簪属植物花瓣中类黄酮化合物价值评价[J]. 草业学报, 2013, 22(1): 234-244.

LIU Ni-na, LI Xiao-dong, ZHANG Jin-zheng, WANG Liang-sheng, SUN Guo-feng, LIN Qin-wen, XU Yan-jun. Flavonoid compounds in the petals of Hosta[J]. Acta Prataculturae Sinica, 2013, 22(1): 234-244.

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