当归炭疽病菌的生物学特性研究

doi:10.11686/cyxb2016295

草业学报 ›› 2017, Vol. 26 ›› Issue (6): 139-144.DOI: 10.11686/cyxb2016295

当归炭疽病菌的生物学特性研究

杨成德¹, 卞静¹, 陈泰祥¹, 陈秀蓉^1,*, 王涵琦¹, 杨小利¹, 王艳²

1.甘肃农业大学植物保护学院, 甘肃兰州 730070;
2.甘肃中医药大学, 甘肃兰州 730000

收稿日期:2016-08-17 修回日期:2016-10-19 出版日期:2017-06-20 发布日期:2017-06-20
通讯作者: E-mail: chenxiurong@gsau.edu.cn
作者简介:杨成德(1975-),男,甘肃武都人,教授,博士。E-mail: yangcd@gsau.edu.cn
基金资助:
甘肃农业大学自列项目(GSAU-ZL-2015-014)资助

Biological characteristics of the Angelica sinensis anthracnose causal agent, Colletotrichum dematium

YANG Cheng-De¹, BIAN Jing¹, CHEN Tai-Xiang¹, CHEN Xiu-Rong^1,*, WANG Han-Qi¹, YANG Xiao-Li¹, WANG Yang²

1.College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China;
2.Gansu University of Chinese Medicine, Lanzhou 730000, China

Received:2016-08-17 Revised:2016-10-19 Online:2017-06-20 Published:2017-06-20

摘要/Abstract

摘要： 本试验利用常规方法研究了当归炭疽病菌(束状炭疽菌Colletotrichum dematium)的生物学特性和营养利用能力,为明确当归炭疽病的发生流行规律提供了依据。结果表明,菌落生长和孢子萌发最低温度和最高温度为10 ℃到35 ℃,最适为25 ℃;在pH 5~11间菌落可以生长,最适pH为11;在pH 8~11间产孢,最适pH为10,但中性有利于孢子萌发;该菌对碳氮源利用范围广,但甘露糖、D-半乳糖和氯醛糖抑制其生长;菌落生长和孢子萌发对光照不敏感;相对湿度95%以上分生孢子能萌发,水中萌发率显著高于其他湿度条件(P<0.05),蔗糖能够促进孢子的萌发,但马铃薯汁液抑制其萌发。

Abstract: The biological characteristics and substrate requirements of Colletotrichum dematium were studied using traditional culture techniques to provide a basis for understanding the occurrence of outbreaks of Angelica sinensis anthracnose. It was found that the temperature range over which mycelium growth and conidial germination of C. dematium occurred was from 10 ℃ to 35 ℃, with an optimum temperature of 25 ℃. Mycelium growth occurred from pH 5 to pH 12, and the optimum pH value was 11. Conidia were produced from pH 8 to pH 11, and the optimum pH value for conidia production was 10, while the optimum for conidial germination was pH 7. The mycelium of C. dematium grew best on media with more abundant carbon and nitrogen supply, but were inhibited by mannitol, D-galactose and chloralose. Mycelium growth and conidial germination were not sensitive to light. Conidia required a relative humidity of 95% or more for germination, and liquid water was the most suitable medium for conidial germination (P<0.05). Sucrose solution promoted conidial germination, but potato solution inhibited germination.

杨成德, 卞静, 陈泰祥, 陈秀蓉, 王涵琦, 杨小利, 王艳. 当归炭疽病菌的生物学特性研究[J]. 草业学报, 2017, 26(6): 139-144.

YANG Cheng-De, BIAN Jing, CHEN Tai-Xiang, CHEN Xiu-Rong, WANG Han-Qi, YANG Xiao-Li, WANG Yang. Biological characteristics of the Angelica sinensis anthracnose causal agent, Colletotrichum dematium[J]. Acta Prataculturae Sinica, 2017, 26(6): 139-144.

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当归炭疽病菌的生物学特性研究

Biological characteristics of the Angelica sinensis anthracnose causal agent, Colletotrichum dematium

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