防治紫花苜蓿真菌病害的生防菌筛选

doi:10.11686/cyxb2016293

草业学报 ›› 2017, Vol. 26 ›› Issue (6): 145-152.DOI: 10.11686/cyxb2016293

防治紫花苜蓿真菌病害的生防菌筛选

胡进玲, 汪治刚, 徐娜, 党淑钟, 李彦忠^*

草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2016-07-19 修回日期:2016-10-09 出版日期:2017-06-20 发布日期:2017-06-20
通讯作者: E-mail: liyzh@lzu.edu.cn
作者简介:胡进玲(1991-),女,甘肃白银人,在读硕士。E-mail: hujl15@lzu.edu.cn
基金资助:
国家自然科学基金(No.31272496),公益性行业(农业)科研专项经费项目(No.201303057), 国家牧草产业技术体系(CARS-35)和云南省高端科技人才引进项目(2012HA012)资助

Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa

HU Jin-Ling, WANG Zhi-Gang, XU Na, DANG Shu-Zhong, LI Yan-Zhong^*

State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2016-07-19 Revised:2016-10-09 Online:2017-06-20 Published:2017-06-20

摘要/Abstract

摘要： 本研究采用革兰氏染色、显微观察和分子生物学方法鉴定了2种具有拮抗潜力的微生物的分类地位,采用PDA培养基平板对峙法测定了其对苜蓿茎点霉(Phoma medicaginis)等9种常见的紫花苜蓿主要病原真菌的拮抗效果。研究结果表明这2种菌分别为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和卡那霉素链霉菌(Streptomyces kanamyceticus)。前者对供试病原菌的抑菌率为24.02%~71.11%,其中对苜蓿尾孢菌(71.11%)、苜蓿匍柄霉(64.76%)和粉红镰孢(60.55%)抑菌效果最强;后者对供试病原菌抑菌率为49.95%~76.90%,其中对三叶草刺盘孢(76.90%)、苜蓿茎点霉(70.94%)、苜蓿匍柄霉(68.01%)和苜蓿小光壳(60.99%)抑制效果较强。两株菌对供试苜蓿9种病原真菌均表现出不同程度的拮抗作用,该研究结果为解淀粉芽孢杆菌和卡那霉素链霉菌作为紫花苜蓿多种病害的生物防治资源以及进一步开发利用提供了理论依据。

Abstract: The inhibitory effect of two bacteria on 9 alfalfa pathogens were tested on PDA medium using the confrontation method; the taxonomic status of each pathogen species were determined using morphological and molecular criteria. The bacteria identified were Bacillus amyloliquefaciens and Streptomyces kanamycin. The inhibitory effect of Bacillus amyloliquefaciens ranged from 24.02%-71.11%, with the greatest effect shown against Cercospora medicaginis (71.11%), Stemphylium botryosum (64.76%) and Fusarium roseum (60.55%). The inhibitory range of Streptomyces kanamycin was similar (49.95%-76.90%) with greatest effect against Colletotrichum trifolii (76.90%), Phoma medicaginis (70.94%), Stemphylium botryosum (68.01%) and Leptosphaerulina briosiana (60.99%). Bacillus amyloliquefaciens and Streptomyces kanamycin showed varying degrees of antagonism to different alfalfa pathogenic fungi indicate that they have promise as biological control agents for a variety of Medicago sativa foliar diseases, suggesting further development should be undertaken.

胡进玲, 汪治刚, 徐娜, 党淑钟, 李彦忠. 防治紫花苜蓿真菌病害的生防菌筛选[J]. 草业学报, 2017, 26(6): 145-152.

HU Jin-Ling, WANG Zhi-Gang, XU Na, DANG Shu-Zhong, LI Yan-Zhong. Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa[J]. Acta Prataculturae Sinica, 2017, 26(6): 145-152.

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防治紫花苜蓿真菌病害的生防菌筛选

Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa

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