海滨雀稗60Co-γ辐射诱变突变体筛选

doi:10.11686/cyxb2016352

草业学报 ›› 2017, Vol. 26 ›› Issue (7): 62-70.DOI: 10.11686/cyxb2016352

海滨雀稗⁶⁰Co-γ辐射诱变突变体筛选

刘天增, 谢新春, 张巨明^*

华南农业大学林学与风景园林学院,广东省草业工程技术研究中心,广东广州 510642

收稿日期:2016-09-18 出版日期:2017-07-20 发布日期:2017-07-20
通讯作者: E-mail:jimmzh@scau.edu.cn
作者简介:刘天增(1984-),男,甘肃永昌人,讲师,博士。E-mail:liutianzeng@scau.edu.cn
基金资助:
广东省科技计划项目(2012B020302002)和国家自然科学基金(31502011)资助

Mutagenic effect of ⁶⁰Co-γ irradiation on turf characteristics of Paspalum vaginatum

LIU Tian-Zeng, XIE Xin-Chun, ZHANG Ju-Ming^*

College of Forestry and Landscape Architecture, South China Agricultural University, Guangdong Engineering Research Center of Grassland Science, Guangzhou 510642, China

Received:2016-09-18 Online:2017-07-20 Published:2017-07-20

摘要/Abstract

摘要： 海滨雀稗具有耐盐、对灌溉水质要求不高的特性。用它建植的草坪可以降低淡水资源的消耗和浪费,但目前我国还没有自己育成的海滨雀稗品种,只能通过国外引进。本实验以海滨雀稗4个品种Sea Isle 2000、Platinum、Supreme、Salam为材料,利用⁶⁰Co-γ射线以0.12 Gy/min 的强度在0,40,45,50,55 Gy 5个剂量下分别照射种茎,从辐照群体中寻找突变材料,对突变材料从叶宽、叶长、株高、匍匐茎节间长度和直径以及密度等坪用性状进行观测分析,研究其植株形态变异的诱变效应,并从中选育性状优良的突变材料。结果表明,材料经辐射后,不同海滨雀稗品种对⁶⁰Co-γ辐射的敏感性不同。经过3个阶段的扩繁、筛选,Sea Isle 2000选出3个突变体,Platinum选出3个突变体,Supreme选出1个突变体,Salam选出2个突变体,初步共选育出9个突变材料。所有突变材料的植株叶宽、叶长、株高、匍匐茎节间长度和直径以及密度等指标均不同程度地优于各自对照。综合来看,突变材料“SI-50-1”、“PL-40-2”、“SLM-45-1”叶片短细、节间缩短、株高矮化、成坪密度高,辐射诱变效果最明显,为新品种选育提供了优异的育种新材料。

Abstract: Seashore paspalum (Paspalum vaginatum) is a salt-tolerant plant that does not require good-quality water for irrigation. Therefore, it has the potential to greatly reduce fresh water consumption and waste. To date, however, no new seashore paspalum cultivars have been bred in China. Mutation is an important method for breeding new varieties of plants, including turfgrass. The objective of this study was to breed new strains of seashore paspalum with the ⁶⁰Co-γ radiation method. Sprigs of “Sea Isle 2000”, “Platinum”, “Supreme”, and “Salam” were radiated at five different radiation intensities, and then favorable mutants were screened by comparing leaf width, leaf length, plant height, stolon length, stolon diameter, and stolon density with those of unirradiated controls. Nine mutants were acquired by ⁶⁰Co-γ irradiation of sprigs of seashore paspalum. Compared with the control plants, the mutants showed greater plant height, leaf length, and leaf width. The turf qualities of the mutants “SI-50-1”, “PL-40-2” and “SLM-45-1” were better than those of the control. In conclusion, we have identified the appropriate radiation intensity of ⁶⁰Co-γ for mutation of seashore paspalum, and we have generated several new mutants of this important plant.

刘天增, 谢新春, 张巨明. 海滨雀稗⁶⁰Co-γ辐射诱变突变体筛选[J]. 草业学报, 2017, 26(7): 62-70.

LIU Tian-Zeng, XIE Xin-Chun, ZHANG Ju-Ming. Mutagenic effect of ⁶⁰Co-γ irradiation on turf characteristics of Paspalum vaginatum[J]. Acta Prataculturae Sinica, 2017, 26(7): 62-70.

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海滨雀稗⁶⁰Co-γ辐射诱变突变体筛选

Mutagenic effect of ⁶⁰Co-γ irradiation on turf characteristics of Paspalum vaginatum

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