利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性

doi:10.11686/cyxb2016374

草业学报 ›› 2017, Vol. 26 ›› Issue (7): 71-81.DOI: 10.11686/cyxb2016374

利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性

涂明月¹, 李杰¹, 何亚丽², 李醒¹, 李俊², 袁晓君^{1, *}

1.上海大学生命科学学院,上海市能源作物育种及应用重点实验室,上海200444;
2.上海交通大学农业与生物学院,上海200240

收稿日期:2016-10-09 出版日期:2017-07-20 发布日期:2017-07-20
通讯作者: E-mail:yuanxjforever@163.com
作者简介:涂明月(1992-),女,安徽淮南人,在读硕士。E-mail:m18817510336@163.com
基金资助:
国家自然科学基金青年科学基金项目(31502002)和沪农科攻字(2013)第1-1号资助

Genetic diversity analysis of Kentucky Bluegrass cultivars by RAPDs

TU Ming-Yue¹, LI Jie¹, HE Ya-Li², LI Xing¹, LI Jun², YUAN Xiao-Jun^{1, *}

1.School of Life Science, Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, China;
2.School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received:2016-10-09 Online:2017-07-20 Published:2017-07-20

摘要/Abstract

摘要： 草地早熟禾是一种重要的冷季型草坪草,广泛种植于温带地区,且拥有众多的商品品种和丰富的野生资源。本研究收集了国内外72份草地早熟禾材料,对它们苗期的株高、叶宽、叶色及分蘖情况进行了考察,结果显示材料间的表型差异显著。此外,又在材料间进行了RAPD扩增产物的多态性鉴定,20条引物共扩增出115条多态性条带,多态性比率达91%。UPGMA聚类显示,材料被聚成两大类:一类为4个国内品种/系(“沪夜”、“沪青”、“沪禾2号”和“杂禾”),另一大类为剩余的所有材料。当遗传相似系数增大后,后一大类分成3组,组Ⅰ以国外引进品种为主,组Ⅱ和组Ⅲ则集中了更多的野生材料。RAPD聚类分析结果与材料背景来源之间存在一定程度的相关,但与苗期表型性状没有相关性。

Abstract: There are many commercial cultivars and wild germplasms of Kentucky bluegrass (Poa pratensis), an important cool-season turfgrass widely cultivated in temperate regions worldwide. In this study, 72 Kentucky bluegrass materials from China and elsewhere were collected and evaluated at the seedling stage by recording plant height, number of tillers, leaf width, and leaf color. All these traits differed significantly among the germplasms. Polymorphic bands were amplified from these germplasms by using random amplified polymorphic DNA (RAPD) primers. A total of 115 polymorphic bands (91%) was generated using 20 RAPD primers. The dendrogram grouped these germplasms into two main clusters. The first cluster contained four domestic varieties (‘KBG04’, ‘KBG03’, ‘Huhe 2’, and ‘Zahe’), while the second cluster contained the other varieties. The second group had a higher genetic similarity coefficient, and was divided into three subgroups: GroupⅠ consisting mainly of imported varieties, and Group Ⅱ and Group Ⅲ with mainly wild germplasms. The clustering analysis based on the RAPDs data was partly consistent with the source of the materials, but there was no correlation with seedling phenotypic traits.

涂明月, 李杰, 何亚丽, 李醒, 李俊, 袁晓君. 利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性[J]. 草业学报, 2017, 26(7): 71-81.

TU Ming-Yue, LI Jie, HE Ya-Li, LI Xing, LI Jun, YUAN Xiao-Jun. Genetic diversity analysis of Kentucky Bluegrass cultivars by RAPDs[J]. Acta Prataculturae Sinica, 2017, 26(7): 71-81.

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利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性

Genetic diversity analysis of Kentucky Bluegrass cultivars by RAPDs

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