甘农三号紫花苜蓿种带细菌的生物功能分析及鉴定

doi:10.11686/cyxb2017107

摘要/Abstract

摘要： 采用稀释平板法从表面消毒的甘农三号紫花苜蓿种子中分离、纯化出8株细菌(ZSR9~16),测定其产IAA、固氮、溶磷和分解纤维素生物学功能,明确6株菌(ZSR9~12和ZSR14~15),它们具有功能多样性,均能产IAA、溶磷、固氮和分解纤维素,菌株ZSR9在它们中的所有生物功能最强;菌株ZSR13具有较弱的产IAA的能力;菌株ZSR16具有较强的产IAA、溶磷、固氮和分解纤维素的能力。通过对这8株菌的菌体形态描述、表型和16S rDNA鉴定,它们分别属于3个属的8种菌。其中芽孢杆菌属(Bacillus)的有6株:枯草芽孢杆菌(B. subtilis) ZSR9、沙福芽孢杆菌(B. safensis) ZSR10、莫海威芽孢杆菌(B. mojavensis) ZSR11、地衣芽孢杆菌(B. licheniformis) ZSR12、短小芽孢杆菌(B. pumilus) ZSR14和深褐芽孢杆菌(B. atrophaeus) ZSR15;ZSR13为肠杆菌属(Enterobacter sp.)和ZSR16为土地芽孢杆菌属(Terribacillus sp.)。这些菌种的得到将为其开发利用奠定菌种资源基础。

Abstract: Eight bacterial strains (ZSR9-16) were isolated from surface-sterilized seeds of Gannong No.3 lucerne using the dilution plate method. Biological functions including indole acetic acid (IAA) production, nitrogen fixation, phosphate solubilization and ability to decompose cellulose were determined for all strains. The results showed that six strains (ZSR9-12 and ZSR14-15) had the ability to produce IAA, solubilize phosphorus, fix nitrogen and decompose cellulose; ZSR9 was better than other strains. Strain ZSR13 had weak IAA production. ZSR16 had good IAA production, phosphorus solubilization, cellulose decomposition and nitrogen fixation. The morphology, phenotype and 16S rDNA sequence of all 8 strains were analyzed and revealed that they belonged to three different genera. Six Bacillus strains included B. subtilis ZSR9, B. safensis ZSR10, B. mojavensis ZSR11, B. licheniformis ZSR12, B. pumilus ZSR14 and B. atrophaeus ZSR15. ZSR13 and ZSR16 belonged to Enterobacter sp. and Terribacillus sp., respectively. These strains will be potentially very useful for improving production of lucerne.

张振粉, 师尚礼. 甘农三号紫花苜蓿种带细菌的生物功能分析及鉴定[J]. 草业学报, 2018, 27(1): 152-160.

ZHANG Zhen-fen, SHI Shang-li. Identification and biological attributes of seed-borne bacteria isolated from lucerne cv. Gannong No.3[J]. Acta Prataculturae Sinica, 2018, 27(1): 152-160.

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