Construction of a bivalent plant expression vector of DREB1A and Bar genes and studies of genetic transformation of potato

doi:10.11686/cyxb20140312

Abstract

Abstract: Potato growth and productivity has been extremely affected by drought-stress. It is important to improve drought tolerance of potato to increase yield under stress conditions. To improve drought resistance of potato, the drought response transcription factor DREBIA gene and rd29A promoter were isolated from Arabidopsis thaliana by PCR. Using recombinant DNA technology, the bivalent plant expression vector pBI121-rd29-BDR, which contains two expression cassettes, rd29A-DREB1A-NOS and CaMV35S-Bar-NOS, was successfully constructed. Potato stems were used for the transformation. Excised stems (0.5-1.0 cm) were infected with Agrobacterium LBA4404/pBI121-rd29-BDR (OD₆₀₀=0.5-0.8) and grown on a co-cultivation medium for 3 days in darkness, then placed on callus induction medium plates containing 2 mg/L PPT and 200 mg/L cefotaxime for callus formation. Twenty two resistant seedlings were obtained. The PCR and RT-PCR testing showed that DREB1A was not only integrated into the genome of the potato Longshu 10, but was also expressed in transgenic plants. To verify drought resistance of transgenic potato, analyses were carried out.

CLC Number:

S532.03

JIA Xiao-xia,QI En-fang,WANG Yi-hang,WEN Guo-hong,GONG Cheng-wen,WANG Hong-mei,LI Jian-wu,MA Sheng,HU Xin-yuan. Construction of a bivalent plant expression vector of DREB1A and Bar genes and studies of genetic transformation of potato[J]. Acta Prataculturae Sinica, 2014, 23(3): 110-117.

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