Abstract: The characteristics of hexaploid somatic mutant CHP2009-14 which was regenerated from embryonic callus treated by colchicine,CHP2009-14 and a triploid hybrid of Tift23A and Pennisetum purpureum cv. Sumu No.2 (CK) were studied. Specific characteristics analysed were morphological traits,plant nutrition value,leaf stomatal characteristics,and ploidy identification by flow cytometry. Compared to those of the CK after 92 days growth in saline soil,the leaf color of CHP2009-14 was dark green,the chlorophyll content increased 10.51% (P<0.05),there were many more hairs in the leaves and sheaths; the plant height was reduced 26.34% (P<0.01); the leaf length decreased by 20.06% (P<0.01) and the leaf thickness increased by 16.41% (P<0.01).The stem/leaf ratio was reduced 43.61% (P<0.01) and number of tillers increased by 44.62% (P<0.01). The leaf width was not significantly different. Digestibility in vitro,crude protein,ash and acid detergent fiber were significantly increased by 6.94% (P<0.05),62.34% (P<0.01),17.35% (P<0.01) and 10.24% (P<0.01),respectively. Neutral detergent fiber decreased by 3.35% and acid detergent fiber increased by 0.50% (P>0.05). Fresh grass yield increased by 0.87% (P>0.05) and dry matter yield decreased by 3.27%(P>0.05). Compared to those of the CK,the stomatal density of CHP2009-14 in the upper and lower epidermis was lower by 65.20% (P<0.01) and 35.71% (P<0.01),the stomatal length and width increased by 47.71% (P<0.01) and 30.53% (P<0.01),51.54% (P<0.01) and 34.17% (P<0.01),respectively,and the stomatal area was 2.07 and 1.54 times that of the CK. Flow cytometry analysis showed that the nuclear DNA contents of CHP2009-14 was twice that of the CK.