Effect of Chinese herbal medicine on milk production, antioxidant capacity and immunity of dairy cows

doi:10.11686/cyxb20150117

Abstract

Abstract: The effect of compound Chinese herbal medicine on milk performance, antioxidant capacity and immunity of dairy cows was studied in 20 healthy mid to late lactation Chinese Holstein cows. Based on lactation time and milk yield, cows were divided into four equal size groups (A, B, C and D). Group A (control) was feed the base diet only (no herbal medicine), whereas group B, C and D were feed the basal diet supplemented with 50, 100 and 150 g/d of the Chinese herbal medicine, respectively. The milk yield for each cow was measured daily. Blood samples for each cow under heat stress conditions were collected on the 1st, 20th and 40th day following implementation of treatments to determine apoptosis rate of lymphocytes, GSH-Px, and gene expression of CAT and lymphocyte apoptosis related gene, Bcl-2, p53. Cows in group B, C and D produced 20.26%, 24.20% and 16.98% more milk than those in group A (P<0.05), respectively. The somatic cell counts in milk from group B, C and D were 21.68%, 29.88%and 24.22% (P<0.05) lower than that group A, respectively. The mean level of GSH-Px and CAT gene expression in group B, C and D was greater than that in group A by 5.00% (P>0.05), 13.00% (P<0.05), 6.00% (P>0.05) and 5.00% (P>0.05), 16.00% (P<0.05), 3.00% (P>0.05), respectively. The apoptosis rate of lymphocyte in cows from group B, C and D were 18.20% (P>0.05), 17.86% (P>0.05) and 12.51% (P>0.05) lower than those in group A, respectively. The expression level of Bcl-2 in group B, C and D was 105.00% (P>0.05), 113.00% (P>0.05) and 57.00% (P>0.05) higher than that in group A, respectively. Additionally, the expression level of p53 gene in groups B, C and D were 24.00% (P>0.05), 28.00% (P>0.05) and 20.00% (P>0.05) lower than that in the control group, respectively. The gene expression level of Bcl-2 and p53 was significantly higher in groups (B, C and D) than that in control group after 20 and 40 days. Within the groups receiving Chinese medicine, milk yield, somatic number, antioxidase activity and expression level of lymphocytic apoptosis related genes were higher in group C than other groups, while the apoptosis rate of lymphocyte in group B was highest. This study suggested that compound Chinese herbal medicine can improve milk performance, antioxidant capacity and immunity of dairy cattle under heat stress.

HU Yongling, YE Shili, LUO Jiajie. Effect of Chinese herbal medicine on milk production, antioxidant capacity and immunity of dairy cows[J]. Acta Prataculturae Sinica, 2015, 24(1): 132-140.

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