Abstract: The herbicide 2,4-D butyl ester was used to control Pedicularis verticilata efficiently. The best time for control of P. verticilata was florescence and the concentration was 0.066 mL/m². The grassland vegetation ecological environment was obviously improved after spraying 2,4-D butyl ester herbicide. P. verticilata was entirely or partially controlled and the high quality forage Elymus multicaulis became dominant. The harmful grassland weed Gentianella turkestanorum was inhibited to some degree. In summary, grassland quality was efficiently improved.