Effects of chlortetracycline and 4-epi-chlortetracycline stress on seedling growth, antioxidant enzyme activities and accumulation in pakchoi

Abstract

Abstract: The effects of different concentrations of chlortetracycline (CTC) and 4-epi-chlortetracycline (ECTC) (1, 10, 30 mg/L) on seed germination, growth, antioxidant enzyme systems, CTC and ECTC accumulation in pakchoi (Brassica campestris ssp. chinensis) seedlings were investigated using a water culture method. The results indicated that root elongation could be recommended as a sensitivity indicator to assess eco-toxicity of CTC and ECTC compared with other targets. The activities of SOD, POD and CAT of pakchoi were enhanced with increasing CTC concentration and the activities of POD and CAT were always higher than that of the control group. Under the same conditions, the SOD, POD and CAT activities were enhanced at a low concentration of ECTC but the latter two were inhibited at high ECTC concentration. CTC and ECTC can accumulate in pakchoi. When the concentration of CTC and ECTC was increased, the contents of CTC and ECTC accumulated in seedlings increased. The experiments revealed that the abilities of pakchoi to accumulate CTC were stronger than for ECTC and the stress was correspondingly greater.

CLC Number:

S634.303.4

WEI Rui-cheng, PEI Yan, ZHENG Xiao-li, ZHU Xu-bo, CHEN Ming, WANG Ran. Effects of chlortetracycline and 4-epi-chlortetracycline stress on seedling growth, antioxidant enzyme activities and accumulation in pakchoi[J]. Acta Prataculturae Sinica, 2011, 20(5): 102-110.

References

European Federation of Animal Health (FEDESA).Antibiotic Use in Farm Animals Does not Threaten Human Health. Brussels, Belgium: FEDESA/FEFANA Press Release, 2001.
Kemper N. Veterinary antibiotics in the aquatic and terrestrial environment. Ecological Indicators, 2008, 8: 1-13.
Union of Concerned Scientists (UCS). Hogging it!: Estimates of Antimicrobial Abuse in Livestock. Washington, DC, USA: UCS Press Release, 2001.
侯放亮. 饲料添加剂应用大全. 北京: 中国农业出版社, 2003.
Alcock R E, Sweetman A, Jones K C. Assessment of organic contaminant fate in wastewater treatment plants I. Selected compounds and physiochemical properties. Chemosphere, 1999, 38: 2247-2262.
Tolls J. Sorption of veterinary pharmaceuticals in soils: a review. Environmental Science & Technology, 2001, 35: 3397-3406.
De Liguoro M, Cibin V, Capolongo F, et al. Use of oxytetracycline and tylosin in intensive calf farming: evaluation of transfer to manure and soil. Chemosphere, 2003, 52: 203-212.
Hamscher G, Sczesny S, Hoper H, et al. Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Analytical Chemistry, 2002, 74: 1509-1518.
Mackie R I, Koike S, Krapac I, et al. Tetracycline residues and tetracycline resistance genes in groundwater impacted by swine production facilities. Animal Biotechnology, 2006, 17: 157-176.
Schmitt H, Stoob K, Hamscher G, et al. Tetracyclines and tetracycline resistance in agricultural soils: microcosm and field studies. Microbial Ecology, 2006, 51: 267-276.
Dolliver H, Kumar K, Gupta S. Sulfamethazine uptake by plants from manure-amended soil. Journal of Environmental Quality, 2007, 36: 1224-1230.
鲍艳宇, 周启星, 谢秀杰. 四环素类抗生素对小麦种子芽与根伸长的影响. 中国环境科学, 2008, 28: 566-570.
Farkas M H, Mojico E E, Patel M, et al. Development of a rapid biolistic assay to determine the changes in relative levels of intracellular calcium in leaves following tetracycline uptake by pinto bean plants. Analyst, 2009,134:1594-1600.
金彩霞, 刘军军, 陈秋颖, 等. 兽药污染土壤对小麦和白菜根伸长抑制的毒性效应.农业环境科学学报, 2009, 28: 1358-1362.
刘吉强, 诸葛玉平, 崔丽娜. 外源四环素对土壤酶活性和油菜品质的影响. 应用生态学报, 2009, 20: 943-948.
魏瑞成, 包红朵, 郑勤, 等. 粪源抗生素金霉素和喹乙醇在养殖水体中的残留及对锦鲤的生态毒理效应研究. 农业环境科学学报, 2009, 28: 1800-1805.
Boxall B A, Johnson P, Smith E J, et al. Uptake of veterinary medicines from soils into plants. Journal of Agricultural and Food Chemistry, 2006, 54: 2288-2297.
Kumar K, Gupta S C, Baidoo S K, et al. Antibiotic uptake by plants from soil fertilized with animal manure. Journal of Environmental Quality, 2005, 34: 2082-2085.
Sengelv G, Halling-Srensen B, Aarestrup F M. Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E. coli from food animals. Veterinary Microbiology, 2003, 95: 91-101.
Teuber M. Veterinary use and antibiotic resistance. Current Opinion in Microbiology, 2001, 4: 493-499.
Xu W H, Zhang G, Li X D, et al. Occurrence and elimination of antibiotics at four sewage treatment plants in the Pearl River Delta (PRD), South China. Water Research, 2007, 41: 4526-4534.
李俊锁, 邱月明, 王超. 兽药残留分析.上海: 上海科学技术出版社, 2002: 393-412.
Xue Y F, Liu L, Liu Z P, et al. Protective role of Ca against NaCl toxicity in Jerusalem artichoke by up-regulation of antioxidant enzymes. Pedosphere, 2008, 18: 766-774.
Martínez-Carballo E, González-Barreiro C, Scharf S, et al. Environmental monitoring study of selected veterinary antibiotics in animal manure and soils in Austria. Environmental Pollution, 2007, 148: 570-579.
王敏, 唐景春. 土壤中的抗生素污染及其生态毒性研究进展. 农业环境科学学报, 2010, 29: 261-266.
秦峰梅, 张红香, 武祎, 等. 盐胁迫对黄花苜蓿发芽及幼苗生长的影响. 草业学报, 2010, 19(4): 71-87.
薛延丰, 李慧明, 易能, 等. 微囊藻毒素(MC-RR)对白三叶种子萌发及幼苗生理生化特性影响. 草业学报, 2009, 18(6): 180-185.
张清智, 陈振德, 王文姣, 等. 毒死蜱胁迫对小白菜抗氧化酶活性和相关生理指标的影响. 生态学报, 2008, 28: 4524-4530.
张永峰, 殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响. 草业学报, 2009, 18(1): 46-50.
Herklotz P A, Gurung P, Vanden Heuvel B, et al. Uptake of human pharmaceuticals by plants grown under hydroponic conditions. Chemosphere, 2010, 78: 1416-1421.
Migliore L, Godeas F, De Filippis S P, et al. Hormetic effect(s) of tetracyclines as environmental contaminant on Zea mays. Environmental Pollution, 2010, 158: 129-134.
McCracken R J, Blanchflower W J, Haggan S A, et al. Simultaneous determination of oxytetracycline, tetracycline and chlortetracycline in animal tissues using liquid chromatography, post-column derivatization with aluminium, and fluorescence detection. Analyst, 1995, 6: 1763-1766.
Jin L, Amaya-Mazo X, Apel M E, et al. Ca²⁺ and Mg²⁺ bind tetracycline with distinct stoichiometries and linked deprotonation. Biophysical Chemistry, 2007, 128: 185-196.
Zia H, Price J C. Binding study of tetracyclines to human serum albumin using difference spectrophotometry. Journal of Pharmaceutical Sciences, 1976, 65: 226-230.
Arikan O A. Degradation and metabolization of chlortetracycline during the anaerobic digestion of manure from medicated calves. Journal of Hazardous Materials, 2008, 158: 485-490.
Posyniak A, Mitrowska K, Zmudzki J, et al. Analytical procedure for the determination of chlortetracycline and 4-epi-chlortetracycline in pig kidneys. Journal of Chromatography A, 2005, 1088: 169-174.