RAPD analysis of Cichorium intybu by space mutagenesis and high-yield strains’ selection

Abstract

Abstract: Cichorium intybu is a excellent forage with high yield and good palatability, which was introduced from abroad.It has been used in production widely. The genetic relationship and production performance of 29 C. intybu were studied in this research by using RAPD method. The result showed that 15 primers selected from 100 random primers were characteristic of high polymorphism, repeatability and stability. 78 DNA fragments were amplified, including 63 polymorphic bands. The average percentage of polymorphic bands was 87.77%, cluster analysis showed that genetic similarity coefficient of C. intybus was ranging from 0.69 to 0.80, there was a lower genetic diversity among germplasm resources of C. intybus tested. PA-186 clustered alone. The fingdings indicated an incomplete correlation among the dendrogram and phenotypic characteristics. According to production performance, the yield of twelve new lines were higher than Puna chicory (9.5 kg/m²)and Jiangjun chicory(9.7 kg/m²).

CLC Number:

HAN Yong-fen, LU Xin-shi, SHU Jian-hong, FU Wei, LU Rui-xia, Qin Tao-ying. RAPD analysis of Cichorium intybu by space mutagenesis and high-yield strains’ selection[J]. Acta Prataculturae Sinica, 2011, 20(5): 133-141.

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