Abstract: The fermentation technology of fuel alcohol manufacturing transformation was studied using an annual ryegrass (Lolium multiflorum) variety for abundant material. It was pretreated with 1% H₂SO₄ and 10% NaOH then hydrolyzed by Trichoderma viride. The relative optimum conditions of program 1 (a volume ratio of Saccharomyces cerevisiae to Pichia pastoris of 7∶3) were a 10% concentration of the yeasts at 37℃, pH 5.5 for 48 h. For program 2 (a ratio of S. cerevisiae, P. pastoris, and Aspergullus niger of 7∶3∶2) a 12% concentration of the mixture at 37℃, pH 5.5 was tested for 48 h. The concentration of strains and the fermentation temperature were the more important factors in both programs. For pretreatments with H₂SO₄ and NaOH, program 2 achieved relatively higher alcohol conversion rates (8.36% and 8.39%, respectively) but there was no significant difference.