Effect of Rhizobium inoculation on crop growth and nitrogen nutrition of a pea/maize intercropping system

Abstract

Abstract: A Rhizobium inoculation experiment was conducted in a pea/maize intercropping system to investigate the changes of nodulation, nitrogen (N) fixation, N absorption, and crop growth. Nitrogenase activity of Rhizobium in pea nodules was increased significantly by intercropping and inoculation with ACCC16101 (R₁) and XC3.1 (R₂). Nodule weights of intercropped pea increased by 19.0%-120.4% after inoculation with these two Rhizobium strains. ACCC16101 had little effect on N absorption and dry matter accumulation of either pea or maize. In an intercropping system inoculated with XC3.1, N and dry matter accumulation of these two crops were considerably improved and seed yields of pea and maize increased by 16.9% and 19.1%, respectively. XC3.1 is suitable for inoculation in a pea/maize system.

CLC Number:

S143.1
S314

GUO Li-zhuo, ZHANG Hu-tian, HE Ya-hui, CHAI Qiang, HUANG Gao-bao. Effect of Rhizobium inoculation on crop growth and nitrogen nutrition of a pea/maize intercropping system[J]. Acta Prataculturae Sinica, 2012, 21(1): 43-49.

References

陈利云, 张海林, 周志宇. 生物与非生物因素对共生固氮的影响. 草业科学, 2010, 27(6): 64-69.
房增国, 赵秀芬, 孙建好, 等. 接种根瘤菌对蚕豆/玉米间作系统氮营养的影响. 华北农学报, 2009, 24(4):124-128.
Van Kessel C, Hartley C. Agricultural management of grain legumes, has it led to an increase in nitrogen fixation. Field Crops Research, 2000, 465: 165-181.
Fan F L, Zhang F S, Song Y N, et al. Nitrogen fixation of faba bean (Vicia faba L.) interacting with a non-legume in two contrasting intercropping systems. Plant and Soil, 2006, 283: 275-286.
Hauggaard-Nielsen H, Jensen E S. Evaluating pea and barley cultivars for complementarity in intercropping at different levels of soil nitrogen availability. Field Crops Research, 2001, 72: 185-196.
Chu G X, Shen Q R, Cao J L. Nitrogen fixation and N transfer from peanut to rice cultivated in aerobic soil in an intercropping system and its effect on soil N fertility. Plant and Soil, 2004, 263: 17-27.
Xiao Y B, Li L, Zhang F S. Effect of root contact on interspecific competition and N transfer between wheat and faba bean using direct and indirect ¹⁵N techniques. Plant and Soil, 2004, 262: 45-54.
Li L, Zhang F S, Li X L, et al. Interspecific facilitation of nutrient uptake by intercropped maize and faba bean. Nutrient Cycling in Agroecosystems, 2003, 65: 61-71.
褚贵新, 沈其荣, 李弈林, 等. 用¹⁵N叶片标记法研究旱作水稻与花生间作系统中氮素的双向转移. 生态学报, 2004, 24(2): 278-284.
Stern W R. Nitrogen fixation and transfer in intercropping system. Field Crops Research, 1993, 34: 335-356.
Sennaratne R, Ratnasinghe D S. Nitrogen supply groundnuts to maize in a maize plus groundnut intercropping system, as affected by the genotype. Biology and Fertility of Soils, 1993, 15: 215-219.
Hauggaard-Nielsen H, Andersen M K, Jornsgaard B, et al. Density and relative frequency effects on competitive interactions and resource use in pea-barley intercrops. Field Crops Research, 2006, 95: 256-267.
刘忠宽, 曹卫东, 秦文利, 等. 玉米-紫花苜蓿间作模式与效应研究. 草业学报, 2009,18(6): 158-163.
王旭, 曾昭海, 朱波, 等. 燕麦与箭筈豌豆不同混作模式对根际土壤微生物数量的影响. 草业学报, 2009, 18(6): 151-157.
左胜鹏, 王会梅, 马永清, 等. 刈割扰动下半干旱区豆科牧草种间关系的反应. 草业学报, 2009, 18(5): 150-159.
Brophy L S, Heichel G H, Russelle M P. Nitrogen transfer forage legumes to grass in a systematic planting design. Crop Science, 1987, 27: 753-758.
褚贵新, 沈其荣, 张娟, 等. 用¹⁵N富积标记和稀释法研究旱作水稻/花生间作系统中氮素固定和转移. 植物营养与肥料学报, 2003, 9(4): 385-389.
Madiama C, Paul L G V. Influence of urea on biological N₂ fixation and N₂ transfer from Azolla intercropped with rice. Plant and Soil, 2003, 250: 105-112.
师尚礼. 甘肃寒旱区苜蓿根瘤菌促生能力影响因子分析及高效促生菌株筛选研究. 兰州: 甘肃农业大学, 2005.
肖焱波, 李隆, 张福锁. 接种不同根瘤菌对间作蚕豆和小麦生长的促进作用研究. 农业现代化研究, 2003, 24(4): 275-277.
钟增涛, 沈其荣, 孙晓红, 等. 根瘤菌在小麦与紫云英混作中的作用. 应用生态学报, 2003, 14(2): 187-190.
房增国, 赵秀芬, 孙建好, 等. 接种根瘤菌对蚕豆/玉米间作系统产量及结瘤作用的影响. 土壤学报, 2009, 46(5): 887-893.
胡博. 不同氮素水平接种对混作的影响初步研究——以玉米紫花苜蓿为例. 北京: 北京林业大学, 2009.
武帆. 菌根真菌和根瘤菌对大豆玉米种间氮素转移作用的研究. 哈尔滨: 东北农业大学, 2009.
武帆, 李淑敏, 孟令波. 菌根真菌、根瘤菌对大豆/玉米氮素吸收作用的研究. 东北农业大学学报, 2009, 40(6): 6-10.
钟增涛, 沈其荣, 冉炜, 等. 旱作水稻与花生混作体系中接种根瘤菌对植株生长的促进作用. 中国农业科学, 2002, 35(3): 303-308.
肖焱波, 李隆, 张福锁. 根瘤菌菌株NM353对小麦/蚕豆间作体系中作物生长及养分吸收的影响. 植物营养与肥料学报, 2006, 12(1): 89-96.
李淑敏, 李隆, 张福锁. 蚕豆/玉米间作接种AM真菌与根瘤菌对其吸磷量的影响. 中国生态农业学报, 2005, 13(3): 136-139.
Ta T C, Faris M A. Effects of environmental conditions on the fixation and transfer of nitrogen from alfalfa to associated timothy. Plant and Soil, 1988,107(1): 25-30.
王平, 王勤, 冯新梅. 华癸根瘤菌在非豆科植物根圈定殖能力的研究. 华中农业大学学报, 1999, 18(3): 238-241.
Ta T C, Faris M A, Macdowall F D H. Evaluation of N¹⁵ methods to measure nitrogen transfer from alfalfa to companion timothy. Plant and Soil, 1989, 114(2): 243-247.
王立祥, 李军. 农作学. 北京: 科学出版社, 2003: 122-123, 152, 177.
陈冠雄, 戴祥鹏, 周礼凯. 生物固氮研究方法. 北京: 科学出版社, 1987: 238, 256.
O’Hara G W. Mineral constraints to nitrogen fixation. Plant and Soil, 1988, 108: 93-110.
鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2005: 264-268.
Mulvaney R L. Nitrogen-inorganic forms. In: Bigham J M. Methods of Soil Analysis, Part 3, Chemical Methods. Madison: SSSA, ASA, 1996: 1123-1124.
Santalla M, Amurrio J M, Rodino A P, et al. Variation in traits affecting nodulation of common bean under intercropping with maize and sole cropping. Euphytica, 2001, 122: 243-255.
李玉英, 孙建好, 李春杰, 等. 施氮对蚕豆/玉米间作系统蚕豆农艺性状及结瘤特性的影响. 中国农业科学, 2009, 42(10): 3467-3474.
Maingi J M, Shisanya C A, Gitonga N M, et al. Nitrogen fixation by common bean (Phaseolus vulgaris L.) in pure and mixed stands in semi-arid south-east Kenya. European Journal of Agronomy, 2001, 14(1): 1-12.
Berdahl J D, Karn J F, Hendrickson J R. Dry matter yield of cool-season grass monocultures and grass-alfalfa binary mixtures. Agronomy Journal, 2001, 93: 463-467.
杜寒春, 郑会明, 周晶, 等. 紫云英-小麦混作体系中氮素转移对小麦生长的促进作用. 土壤学报, 2006, 43(6): 1043-1046.

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