Biological characteristics of a pathogen of turfgrass root rot disease

Abstract

Abstract: The chief pathogen was identified as Pythium aphanidermatum and its morphology, cultural characteristics and pathogenicity were investigated. The optimal temperatures for fungal growth were 30-35℃ but it could grow in the range 15-40℃.The temperature range for sporangium production was 15-25℃. The best pH was 7-8 for hyphal growth, 9-10 for sporangium development, six for oospore development. The best cultural condition for mycelial and oospore growth was 24 h in the dark while the optimum treatment for sporangium growth was 24 h under light. Ultraviolet irradiation can accelerate the growth of hyphae and sporangia but it inhibits oospore production. The best source of carbon was amylum and the best source of nitrogen was ammonium nitrate.

CLC Number:

S435.4

GU Li-jun, XU Bing-liang, LI Bin, LIANG Qiao-lan. Biological characteristics of a pathogen of turfgrass root rot disease[J]. Acta Prataculturae Sinica, 2012, 21(3): 93-98.

References

张玉琴, 王代军, 刘荣堂, 等. 草坪草腐霉枯萎病的研究现状及进展. 草原与草坪, 2002, (2): 3-7.
Abad Z G, Shew H D, Lucas L T. Characterization and pathogenicity of Pythium species isolated from trufgrass with symptoms of root and crown rot in North Carolina. Phytopathology, 1994, 84: 913-921.
石洁, 李建成, 刘玉瑛. 草坪草根腐病病原菌研究初报. 华北农学报, 2000, 15(增刊): 94-98.
穆合塔尔, 王瑞, 刘荣堂. 多菌灵防治草坪草腐霉枯萎病的研究. 草原与草坪, 2000, (2): 22-25.
李崴, 李秀文, 栗振华, 等. 天津草坪病害诊断技术研究初报. 天津农林科技, 2003, (4): 12-14.
张金林. 坪草腐霉病菌除草活性毒素组分的分离鉴定与突变菌株的 DDRT 差异显示分析. 保定: 河北农业大学, 2005.
张金林, 庞民好, 刘颖超, 等. 坪草腐霉病菌毒素产生除草活性物质的条件优化. 河北农业大学学报, 2005, 28(4): 84-88.
杨亚君, 陶晡, 李川, 等. 腐霉枯萎病菌毒素在坪草抗病性筛选中的应用研究. 草业学报, 2008, 17(3): 93-98.
徐娇, 许文超, 康占海, 等. 瓜果腐霉毒素对多年生黑麦草的致病作用机理初步研究. 草业学报, 2009, 18(4): 181-186.
古丽君, 徐秉良, 梁巧兰, 等. 生防木霉菌T2菌株对禾草腐霉病抑菌作用及机制研究. 草业学报, 2011, 20(2): 46-51.
徐娇, 陈红岩, 石文伟, 等. 坪草腐霉枯萎病菌的生物学特性及诱变菌株毒素的除草活性研究. 河北农业大学学报, 2008, 31(4): 81-86.
余永年, 马国忠. 腐霉的生长温度与分类. 云南农业大学学报, 1990, 5(2): 65-71.
徐娇, 陈红岩, 石文伟, 等. 坪草腐霉枯萎病菌(Pythium aphanidermatum)的生物学特性及诱变菌株毒素的除草活性研究. 云南农业大学学报, 2008, 31(4): 81-86.
孙淑琴, 刘水芳, 杨秀荣. 天津地区草坪腐霉枯萎病病原鉴定及生物学特性研究. 草原与草坪, 2009, (3): 31-38.
古丽君, 徐秉良, 梁巧兰, 等. 兰州市草坪禾草根腐病的发生及病原菌鉴定. 草业学报, 2009, 18(4): 175-180.
徐秉良, 郁继华. 非洲菊枯萎病菌的生物学特性. 甘肃农业大学学报, 2002, 37(1): 35-39.
方中达. 植病研究方法. 北京: 中国农业出版社, 1998.
张俊忠, 陈秀蓉, 杨成德, 等. 东祁连山高寒草地土壤可培养真菌多样性分析. 草业学报, 2010, 19(2): 124-132.
郑小波. 疫霉菌及其研究技术. 北京: 中国农业出版社, 1997.
张金林, 庞民好, 刘颖超, 等. 不同杀菌剂对草坪草病原菌毒力的作用测定. 草业学报, 2006, 15(1): 58-61.
王国良. 影响冷季型草坪腐霉病发生因子的研究. 浙江农业学报, 2000, 12(3): 136-139.
谭鹏, 张学忠. 草坪主要病害的发生与防治. 陕西林业科技, 2005, (2): 41-44.
顾爱星, 范燕敏, 武红旗, 等. 天山北坡退化草地土壤环境与微生物数量的关系. 草业学报, 2010, 19(2): 116-123.

[1]	MA Hui-ling,FANG Yuan-yuan. Induction of plant disease resistance and its application for disease control in creeping bentgrass [J]. Acta Prataculturae Sinica, 2014, 23(5): 312-320.
[2]	NIE Hong-xia,GAO Feng,DUAN Ting-yu,LI Yan-zhong. Progress in research on the diseases of Onobrychis viciaefolia [J]. Acta Prataculturae Sinica, 2014, 23(3): 302-312.
[3]	ZHU Meng-meng, LIU Yan, ZHANG Rong, HUANG Wen-guang. Canonical correlations between pests and natural enemies and their niches in alfalfa grasslands [J]. Acta Prataculturae Sinica, 2013, 22(6): 159-166.
[4]	XIANG Hong-yan, LIU Ai-ping, GAO Shu-jing, XU Lin-bo, LIU Chang-zhong. A biological study on Exorista civilis, a tachinid parasitoid of Loxostege sticticalis [J]. Acta Prataculturae Sinica, 2013, 22(3): 92-.
[5]	WEN Ke-jian, LUO Tian-qiong, ZHANG Li, CHEN Yan-ping, ZHOU Yu-feng, LONG Zhong-fu,WU Jia-hai, SU Sheng . Control efficacy of 6 fungicides against 3 pathogens of turfgrass diseases [J]. Acta Prataculturae Sinica, 2013, 22(3): 124-.
[6]	LIU Jin-ping, YOU Ming-hong. Effect of intrusion volume of Imperata koenigii on relative competitiveness and traits of young Festuca arundinacea turf [J]. Acta Prataculturae Sinica, 2012, 21(6): 315-320.
[7]	LV Zong-you, SU Yan-jing, ZHAO Guo-qi, WU Cai-xia. Effects of anti-browning agents on the induction of sudangrass callus [J]. Acta Prataculturae Sinica, 2011, 20(3): 174-181.
[8]	LI Yan-zhong, NAN Zhi-biao, ZHANG Zhi-xin, LIU Yong-ru, GAO Xin-ye, ZHEN Jian-xun. Distribution of and damage caused by yellow stunt and root rot of Astragalus adsurgens in five northern provinces of China [J]. Acta Prataculturae Sinica, 2011, 20(2): 39-45.
[9]	GU Li-jun, XU Bing-liang, LIANG Qiao-lan, LI Rong-feng. Antagonism and mechanism of action of Trichoderma aureoviride against Pythium aphanidermatum causing turfgrass root rot [J]. Acta Prataculturae Sinica, 2011, 20(2): 46-51.
[10]	ZHOU Hong-xu, LI Chang-you, LI Guo-xun. Molecular cloning and sequencing of cDNAs of the novel protein Ho-Peritrophin3 from the peritrophic matrix of Holotrichia oblita, a turfgrass pest [J]. Acta Prataculturae Sinica, 2010, 19(6): 147-153.
[11]	GU Li-jun, XU Bing-liang, LIANG Qiao-lan, XUE Ying-yu. Occurrence of Pythium root rot in turf grass and identification of the pathogen [J]. Acta Prataculturae Sinica, 2009, 18(4): 175-180.
[12]	XU Jiao, XU Wen-chao, KANG Zhan-hai, ZHENG Hui, ZHANG Jin-lin. A primary study on the pathogenesis of Pythium aphanidermatum to Lolium perenne [J]. Acta Prataculturae Sinica, 2009, 18(4): 181-186.