Effect of waterlogging on antioxidant and anaerobic respiratory enzymesin Medicago sativa varieties from southern and northern China

doi:10.11686/cyxb20150510

Abstract

Abstract: Two Medicago sativa varieties, Yumu No.1 (southern China) and Xinjiangziniquan (northern China), were compared. Six waterlogging treatments; 0, 2, 4, 6, 8 and 10 days of waterlogging were imposed on seedlings at the 4-leaf stage. The malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity in the leaves and lactate dehydrogenase (LDH), pyruvate (PDC) and alcohol dehydrogenase (ADH) activity in the roots were evaluated in all treatments. Biomass was measured after 10 days of waterlogging. Waterlogging stress reduced the biomass of both varieties but Yumu No.1 was less affected than Xinjiangziniquan. The activities of SOD, POD and CAT in leaves of Yumu No.1 were higher than those of Xinjiangziniquan under waterlogging stress, but the MDA content in the leaves was lower than that of Xinjiangziniquan. The activity of respiratory enzymes in the roots was influenced by waterlogging; In Yumu No.1 ADH and PDC activity increased more than LDH activity with stronger alcohol dehydrogenaseactivity, while in Xinjiangziniquanwaterlogging resulted in increased LDH and lactic dehydrogenase activity. It was concluded that the greater tolerance of waterlogging observed in Yumu No.1 was primarily due to higher antioxidant enzyme and anaerobic respiratory enzyme activity.

QUAN Rui-Lan, YU Yong-Xiong. Effect of waterlogging on antioxidant and anaerobic respiratory enzymesin Medicago sativa varieties from southern and northern China[J]. Acta Prataculturae Sinica, 2015, 24(5): 84-90.

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