Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar

doi:10.11686/cyxb2014533

Abstract

Abstract: In order to compare drought resistance of DREB1A/Bar transgenic potato plants, with a control cultivar (non-transgenic Longshu 10), 5-7 cm seedlings of DREB1A transgenic lines were grown in pots using vermiculite and regularly watered. Watering continued to the 15-16 leaf stage after which plants were not watered for 18 days to impose for drought stress. During the drought stress, plant phenotypic features were recorded using visual observation and digital camera images. After 10 days of drought stress, stress-related physiological and biochemical parameters were determined. During the normal watering phase both the non-transgenic control and the transgenic potato lines grew well, the MDA content, relative electrical conductivity and superoxide dismutase (SOD) activity were not significantly different (P>0.05). After 10 days of drought stress the control plants began to wilt, but the transgenic lines remained in good condition. MDA content and relative electrical conductivity in the transgenic line was significantly lower than those of the non-transgenic plants (P<0.05), while the SOD activity was significantly higher than that of non-transgenic plants (P<0.05). After for 18 days of drought stress 74.33% of the control plants were dead whereas fewer transgenic plants had died; the mortality of DR2 and DR5 was 20.43% and 5.65% respectively. The resistance of DREB1A/Bar transgenic potatoes to glufosinate, a systemic non-selective herbicide, compared with non-transgenic lines was determined by treating plants with a 0.3% glufosinate spray. Ten days after treatment all non-transgenic plants had died, while the transgenic plants were affected slightly. The study indicated that introduction of DREB1A and Bar genes significantly enhanced drought tolerance and herbicide resistance in transgenic potato plants.

JIA Xiao-Xia, QI En-Fang, MA Sheng, HU Xin-Yuan, WANG Yi-Hang, WEN Guo-Hong, GONG Cheng-Wen, LI Jian-Wu. Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar[J]. Acta Prataculturae Sinica, 2015, 24(11): 58-64.

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