Pathogenic mechanism of Rhizoctonia solani potato blight Ⅰ Micro-structure observation of the infection process and measurement of cell wall degradation enzyme activity

doi:10.11686/cyxb2015051

Abstract

Abstract: Stem canker (black scurf disease) caused by Rhizoctonia solani has become one of the main soil-borne diseases which limit the development of the potato industry in Gansu province. In this study, R. solani spores were inoculated onto the stem base of potato plants grown in pots. The infection process and activity of potato cell wall degradation enzymes was observed and measured. Results showed that the infection sequence was bud, stem, root, stolon and lastly tuber. Hyphae attached to the surface of the host within 12 h, appressoria formed in 36 h, infection cushions formed in 48 h. Deformation and fracture of the cell wall, damage to the cell membrane, and the effects of plasmolysis such as deformation and fracture of the cell wall, damage to the cell and plastid membranes and the dissolution of cytoplasm were then observed under the electron microscope. The activities of cellulase (Cx), polygalacturonase (PG) and polymethyl-galacturonase (PMG) were 1.7, 2.9 and 3.1 μg/(h·g), respectively, and these values were significantly higher than those of the control group. The activities of PG and PMG were as twice those of the control group. The increased enzyme activity may be related to tissue necrosis.

TUO Ning, ZHANG Jun, QIU Hui-Zhen, ZHANG Wen-Ming, ZHANG Chun-Hong, LIU Xing, ZHU Jing. Pathogenic mechanism of Rhizoctonia solani potato blight Ⅰ Micro-structure observation of the infection process and measurement of cell wall degradation enzyme activity[J]. Acta Prataculturae Sinica, 2015, 24(12): 74-82.

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