Analysis of genetic diversity in Sorghum germplasm collections using SSR markers

doi:10.11686/cyxb2015336

Abstract

Abstract: Fifteen SSR markers were used to quantify the genetic diversity of the core germplasm collections of sorghum. A total of 161 accessions were assayed. 118 bands were generated, among which 88 (75.21%) were polymorphic. The average value of polymorphic information content (PIC) was 0.699 (0.612-0.806); the average Nei’s gene diversity index (H) was 0.696; and the average Shannon information index (I) was 1.393. The genetic similarities among the 161 accessions ranged from 0.636 to 0.977. The UPGMA dendrogram indicated that the 161 Sorghum germplasm collections were clustered into three groups with a threshold of 0.682, and that the 129 Sorghum sudanense and Sorghum bicolorx×Sorghum sudanense germplasm resources were divided into two clusters at a threshold of 0.671. This study demonstrated that there is considerable genetic diversity conserved in Sorghum germplasm collections and SSR is an ideal molecular marker for assessing this diversity.

WANG Fang, GAO Qiu, WANG Jie, MA Jin-Xing, SUN Juan. Analysis of genetic diversity in Sorghum germplasm collections using SSR markers[J]. Acta Prataculturae Sinica, 2016, 25(5): 125-133.

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