Observations and study on the pollen morphology of some taxa of Miscanthus (Poaceae)

Abstract

Abstract: Miscanthus (Gramineae, Poaceae) has recently received comprehensive attention because of its great potential to be developed and utilized as a new renewable energy plant. To accumulate information about pollen morphology of Miscanthus in China for relevant study, the pollen morphology of 39 accessions from Miscanthus sinensis, M. floridulus, M. sacchariflorus, M. lutarioriparius, M. nepalensis, and M. nudipes were observed using light and scanning electron microscopy. The exine sculpture of pollen grains of Miscanthus was of two types: granulate and verrucate. The latter could be divided into three types: obscurely verrucate, verrucate, and obviously verrucate based on differences of the raised extent of verrucation. There were distinct differences in the exine ornamentation and pollen size between the group containing M. nepalensis and M. nudipes and the group consisting of M. sinensis, M. floridulus, M. sacchariflorus, and M. lutarioriparius, supporting the separation of M. nepalensis and M. nudipes from Miscanthus as a standalone genus, Diandranthus. M. sinensis, M. floridulus, M. sacchariflorus, and M. lutarioriparius shared a high similarity in pollen morphology, showing the close affinity between them. Meanwhile, M. floridulus was more advanced than M. sinensis in evolutionary history. Nevertheless, it may be essential to further investigate the exine structure of pollen grains of Miscanthus in order to clarify the phylogenetic relationship and evolutionary level between the taxa of Miscanthus based on pollen morphology.

CLC Number:

Q944.42

LU Yu-fei, JIANG Jian-xiong, AI Xin, XIAO Liang, YI Zi-li. Observations and study on the pollen morphology of some taxa of Miscanthus (Poaceae)[J]. Acta Prataculturae Sinica, 2012, 21(6): 151-158.

References

[1] Hodkinson T R, Chase M W, Lledó M D, et al. Phylogenetics of Miscanthus,Saccharum and related genera (Saccharinae, Andropogoneae, Poaceae) based on DNA sequences from ITS nuclear ribosomal DNA and plastid trnL intron and trnL-F intergenic spacers[J]. Journal of Plant Research, 2002, 115(5): 381-392.
[2] Chen S L, Renvoize S A. Miscanthus[A]. In: Wu Z Y, Raven P H. Flora of China[M]. Beijing & St.Louis: Science Press;Missouri Botanical Garden Press, 2006: 581-583.
[3] Jakob K, Zhou F S, Paterson A H. Genetic improvement of C₄ grasses as cellulosic biofuel feedstocks[J]. In Vitro Cellular & Developmental Biology - Plant, 2009, 45(3): 291-305.
[4] Sang T, Zhu W X. China’s bioenergy potential[J]. GCB Bioenergy, 2011, 3(2): 79-90.
[5] 刘明稀, 蒋建雄, 易自力. 细胞工程技术在芒属能源作物上的应用[J]. 草业学报, 2011, 20(4): 261-269.
[6] 侯新村, 范希峰, 武菊英, 等. 纤维素类能源草在京郊地区的经济效益与生态价值评价[J]. 草业学报, 2011, 20(6): 12-17.
[7] 刘亮. 芒属, 双药芒属, 荻属[A]. 见: 陈守良, 庄体德, 方文哲, 等. 中国植物志[M]. 北京: 科学出版社, 1997: 4-26.
[8] 孙必兴, 王松. 禾本科甘蔗亚族一新属[J]. 云南植物研究, 1997, 19(3): 239-240.
[9] Sun Q, Lin Q, Yi Z L, et al. A taxonomic revision of Miscanthus s.l. (Poaceae) from China[J]. Botanical Journal of the Linnean Society, 2010, 164: 178-220.
[10] Huang T C. Pollen grains of formosan plants (6)[J]. Taiwania, 1970, 15(1): 73-179.
[11] 于慧, 胡晓颖, 徐信兰, 等. 须芒草族植物花粉形态的观察[J]. 热带亚热带植物学报, 2000, 8(2): 164-170, 186-187.
[12] 蓝盛银, 徐珍秀. 植物花粉剥离观察扫描电镜图解[M]. 北京: 科学出版社, 1996: 3-12.
[13] Ibaragi Y. The taxonomy of Diandranthus (Poaceae)[J]. Acta Phytotaxonomica Geobotanica, 2003, 54(2): 109-125.
[14] 埃尔特曼 G. 孢粉学手册[M]. 北京: 科学出版社, 1978: 6-115.
[15] 王开发, 王宪曾. 孢粉学概论[M]. 北京: 北京大学出版社, 1983: 21-34.
[16] 王伏雄, 钱南芬, 张玉龙, 等. 中国植物花粉形态[M]. 北京: 科学出版社, 1997: 3-9, 38-47, 206.
[17] 张彦妮, 钱灿. 12种百合属植物花粉形态扫描电镜观察[J]. 草业学报, 2011, 20(5): 111-118.
[18] Lafferty J, Lelley T. Cytogenetic studies of different Miscanthus species with potential for agricultural use[J]. Plant Breeding, 1994, 113: 246-249.
[19] 何立珍, 李爱华, 刘选明, 等. 南荻的染色体核型模式[J]. 湖南农业大学学报, 1996, 22(5): 461-465.
[20] 陈少风, 何俊, 周朴华, 等. 芒和五节芒的核型研究[J]. 江西农业大学学报, 2008, 30(1): 123-126.
[21] Mehra P N, Sharma M L. Cytological studies in some central and eastern Himalayan grasses: Ⅰ. The Andropogoneae[J]. Cytologia, 1975, 40: 61-74.
[22] Chou C H, Ueng J J. Phylogenetic relationship among species of Miscanthus populations in Taiwan[J]. Botanical Bulletin of Academia Sinica, 1992, 33: 63-73.