Establishment of a gametocidal chromosome 2C specific SCAR marker

Abstract

Abstract: A total of 40 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on common wheat CS (Chinese spring), CS-2C disomic addition line, Aegilops cylindrica. Of these primers, one could amplify a specific DNA fragment in CS-2C disomic addition line and A. cylindrica. The fragment OPF03₁₄₉₆ was cloned and sequenced; Sequence data searching in GenBank showed that this fragment was 92% homologous to a cloned cDNA sequence from Barley. Based on the sequence of OPF03₁₄₉₆, a pair of SCAR (sequence characterized amplified region) primer 2C-F₅₈₆, 2C-R₅₈₆ was designed. SCAR analyses were performed on CS, CS-2C disomic addition line, A. cylindrica, and Thinopyrum elongatum (2X) CS-7E disomic addition line. The amplification results of SCAR primer showed that the SCAR₅₈₆ marker appeared in the CS-2C disomic addition line and A. cylindrica but not in CS, T. elongatum (2X) CS-7E disomic addition line. Furthermore, SCAR analyses were also performed on F₁ and F₂ progenies between CS-2C and CS-7E disomic addition lines. PCR amplification results showed that the SCAR₅₈₆ marker was in all of the F₁ progenies between CS-2C and CS-7E and in some of the F₂ progenies. The SCAR₅₈₆ marker can be used for detecting the gametocidal chromosome 2C in common wheat backgrounds.

CLC Number:

XU Guo-hui, GUO Chang-hong, YU Hong-tao, CHEN Jing, CONG Wen-wen, ENDO T R. Establishment of a gametocidal chromosome 2C specific SCAR marker[J]. Acta Prataculturae Sinica, 2011, 20(4): 305-310.

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