Isolation and identification of high-quality lactic acid bacteria from wild forage silages on the Hulunbuir prairie

doi:10.11686/cyxb2016092

Abstract

Abstract:

The objectives of this study were to identify lactic acid bacteria (LAB) from forage silages on the Hulunbuir prairie and screen LAB for desirable attributes. LAB strains were isolated from grass silages of four different communities and identified with classical identification and 16S rRNA analysis methods. Five strains: DME53, DMG108, DMC80, DMG138 and DMG139 were isolated and identified as Lactobacillus graminis, L. casei, L. plantarum, L. brevis and Pediococcus pentosaceus, respectively, in which DMG138 was identified as heterofermentative LAB while the other four strains were all homofermentative LAB. All five strains grew well at different temperatures, ranging from 5 to 45 ℃, and in 3.0% and 6.5% NaCl; an exception was that DMG139 grew poorly at 40 and 45 ℃. All strains generally grew well at pH ranging from 3.0-8.0; however, DME53 was inactive at pH 3.0. In conclusion, strain DMC80 demonstrated consistently desirable traits; strong acid production capacity, rapid fermentation rate and good tolerance of acidity and cold which could be used for the preparation of silage in Hulunbuir prairie.

WANG Hong-Mei, SUN Qi-Zhong, TU Yan, SI Bing-Wen, SA Ru-La, NA Ya, DIAO Qi-Yu. Isolation and identification of high-quality lactic acid bacteria from wild forage silages on the Hulunbuir prairie[J]. Acta Prataculturae Sinica, 2016, 25(8): 189-196.

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