Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa

doi:10.11686/cyxb2016293

Abstract

Abstract: The inhibitory effect of two bacteria on 9 alfalfa pathogens were tested on PDA medium using the confrontation method; the taxonomic status of each pathogen species were determined using morphological and molecular criteria. The bacteria identified were Bacillus amyloliquefaciens and Streptomyces kanamycin. The inhibitory effect of Bacillus amyloliquefaciens ranged from 24.02%-71.11%, with the greatest effect shown against Cercospora medicaginis (71.11%), Stemphylium botryosum (64.76%) and Fusarium roseum (60.55%). The inhibitory range of Streptomyces kanamycin was similar (49.95%-76.90%) with greatest effect against Colletotrichum trifolii (76.90%), Phoma medicaginis (70.94%), Stemphylium botryosum (68.01%) and Leptosphaerulina briosiana (60.99%). Bacillus amyloliquefaciens and Streptomyces kanamycin showed varying degrees of antagonism to different alfalfa pathogenic fungi indicate that they have promise as biological control agents for a variety of Medicago sativa foliar diseases, suggesting further development should be undertaken.

HU Jin-Ling, WANG Zhi-Gang, XU Na, DANG Shu-Zhong, LI Yan-Zhong. Screening of inhibitory bacteria for prevention and treatment of fungal diseases of Medicago sativa[J]. Acta Prataculturae Sinica, 2017, 26(6): 145-152.

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