Transcriptome profiling analysis of the phosphate-solubilizing mechanism of the white clover rhizosphere strain RW8

doi:10.11686/cyxb2016399

Abstract

Abstract: We conducted transcriptome sequencing of the white clover (Trifolium repens) rhizosphere phosphorus-solubilizing bacterium RW8 to explore its gene expression profiles. Cells of RW8 were cultured in three different media: one containing insoluble phosphorus (group A), one containing soluble phosphorus (group B), and one lacking phosphorus (group C). Compared with group B cells, group A cells showed 4782 and 447 up- and down-regulated genes, respectively, and group C cells showed 3630 and 209 up- and down-regulated genes, respectively. The up-regulated genes in Groups A and C had similar gene ontology annotations. The main subcategories in the biological process category were metabolic process, cellular process, single-organism process, response to stimulus, localization, and biological regulation. In the cell components category, the main subcategories were cell part, membrane, membrane part, and macromolecular complex. In the molecular function category, the main subcategories were catalytic, binding, and transporter. A metabolic pathway analysis showed that several metabolic pathways were significantly enriched in groups A and C, including 2-oxocarboxylic acid metabolism, alpha-linolenic acid metabolism, C5-branched dibasic acid metabolism, fatty acid metabolism, glycine-serine-threonine metabolism and valine-leucine-isoleucine biosynthesis. Ten differentially expressed genes in three different groups were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The transcriptional patterns detected by qRT-PCR were similar to those detected in the transcriptome sequencing analyses. The composition and concentrations of organic acids were detected by high performance liquid chromatography (HPLC). The lactic acid, succinic acid, and citric acid concentrations were significantly higher in group A and group C than in group B. The fumaric acid and malic acid concentrations were much higher in group C than in groups A and B, and did not differ significantly between groups A and B. The concentration of α-ketoglutaric acid was higher in group A than in group B, and did not differ significantly between groups C and B.

LI Xiao-Dong, WANG Xiao-Li, CHEN Xi, CAI Lu, ZENG Qing-Fei, SHU Jian-Hong, CAI Yi-Ming. Transcriptome profiling analysis of the phosphate-solubilizing mechanism of the white clover rhizosphere strain RW8[J]. Acta Prataculturae Sinica, 2017, 26(8): 168-179.

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