Effects of tannic acid on digestion and utilization of nutrients and the metabolic parameters of nitrogen in sheep

doi:10.11686/cyxb2017135

Abstract

Abstract: An experiment was conducted to determine the effects of tannic acid on nutrient digestion and nitrogen metabolic parameters in sheep, in order to determine the appropriate amount of tannic acid in feed. Four fine-wool wethers (two years old and average live weight of 35±2.15 kg) fitted with permanent rumen fistula were selected and a 4×4 Latin square design with 10 d preliminary and 6 d sampling experiment was used to study the effects of 4 different tannic acid contents on nutrient digestion and nitrogen metabolic profiles. The contents of tannic acid in diets were 0 (A), 10 (B), 15 (C), and 20 (D) g/kg DM. The results indicated that both feed intake and the intake of DM, OM, NDF and ADF were affected by the tannic acid in treatment D (20 g/kg DM) (P>0.01), while the digestibility of DM, OM, NDF, ADF, Ca and P was unaffected by the acid contents (P>0.05). The intake and digestion of N were higher in the A, B and C treatments than in treatment D (P>0.01). N retention increased as the level of tannic acid increased (N retention in treatment C was higher than in treatment A). No differences were observed in N digestibility, pH and total nitrogen in rumen fluids (P>0.05), whereas the levels of NH₃-N and BUN decreased linearly. The level of NH₃-N for diet A was higher than for D (P<0.05) and BUN was higher in the C and D treatments (P>0.01). In conclusion, tannic acid increased rumen bypass proteins. N retention was improved when the ratio of tannic acid was less than 15 g/kg DM, suggesting that the optimal tannic acid content in diets is less than this ratio.

PAN Fa-Ming, WANG Cai-Lian, DIAO Qi-Yu, SONG Shu-Zhen, LANG Xia, GONG Xu-Yin, ZHANG Jun-Cheng. Effects of tannic acid on digestion and utilization of nutrients and the metabolic parameters of nitrogen in sheep[J]. Acta Prataculturae Sinica, 2017, 26(12): 179-185.

References

[1] Xu X F, Zhang L L. Nutritional role of tannin for ruminants. Chinese Journal of Animal Nutrition, 2011, 23(12): 2084-2089.
徐晓锋, 张力莉. 单宁对反刍动物促营养作用的研究进展. 动物营养学报, 2011, 23(12): 2084-2089.
[2] Li Z F, Gao T Y, Zhou C S. Nutritional mechanism of tannin on nutrients utilization in ruminants. Acta Ecologiae Animalis Domastici, 2007, 28(6): 97-103.
黎智峰, 高腾云, 周传社. 单宁对反刍动物养分利用的营养机制. 家畜生态学报, 2007, 28(6): 97-103.
[3] Getachewn G, Makkar H P S, Becker K. Method of polyethylene glycol application to tannin-containing browses to improve microbial fermentation and efficiency of microbial protein synthesis from tannin-containing browes. Animal Feed Science and Technology, 2001, 92(1): 51-57.
[4] Yang Y F, Wei H J. Review of effects of tannins on ruminants. Special Wild Economic Animal and Plant Research, 2010, 32(1): 60-64.
杨镒峰, 魏海军. 单宁对反刍动物影响的研究进展. 特产研究, 2010, 32(1): 60-64.
[5] Zhang X Q, Li Y, Li F D, et al . Effects of tannin content in sainfoin ( Onobrychis viciifolia ) hay on digestibility of nutrients and utilization of nitrogen in sheep diets. Acta Veterinaria et Zootechnica Sinica, 2009, 40(3): 356-362.
张晓庆, 李勇, 李发弟, 等. 红豆草单宁含量对绵羊养分消化率及氮利用的影响. 畜牧兽医学报, 2009, 40(3): 356-362.
[6] Yang S X. Evaluation Method of Feed Ntritional Vlue. Lanzhou: Gansu People's Press, 1982.
杨诗兴. 饲料营养价值评定方法. 兰州: 甘肃人民出版社, 1982.
[7] Yang S. Feed and Feed Quality Detection Technology. Beijing: Beijing Agricultural University Press, 1993.
杨胜. 饲料分析及饲料质量检测技术. 北京: 北京农业大学出版社, 1993.
[8] Feng Z C. Improvement of ammonia nitrogen content in rumen by colorimetry. Inner Mongolia Animal Husbandry Science, 1993, (4): 40-41.
冯宗慈. 通过比色法测定瘤胃液氨氮含量方法的改进. 内蒙古畜牧科学, 1993, (4): 40-41.
[9] Waghorn G C, Shelton I D, Mcnabb W C. Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep.1. Non-nitrogenous aspects. Journal of Agricultural Science, 1994, 123(1): 109-119.
[10] Stienezen M, Waghorn G C, Douglas G B. Digestibility and effects of condensed tannins on digestion of sulla ( Hedysarum coronarium ) when fed to sheep. New Zealand Journal of Agricultural Research, 1996, 39(2): 215-221.
[11] Barahona R, Lascano C E, Cochran R, et al . Intake, digestion, and nitrogen utilization by sheep fed tropical legumes with contrasting tannin concentration and astringency. Animal Science, 1997, 75(6): 1633-1640.
[12] Zhang X Q. Effects of Tannin in Sainfoin on Protein Protection of Diets. Lanzhou: Gansu Agricultural University, 2005.
张晓庆. 红豆草中单宁对饲粮蛋白质保护的效果. 兰州: 甘肃农业大学, 2005.
[13] Pan F M, Li F D, Hao Z L, et al . Effects of tannin content in residue of tea-leaves on digestion and utilization of nutrients and metabolic parameters of nitrogen in sheep. Acta Veterinaria et Zootechnica Sinica, 2012, 43(1): 71-81.
潘发明, 李发弟, 郝正里, 等. 茶渣单宁含量对绵羊养分消化利用与氮代谢参数的影响. 畜牧兽医学报, 2012, 43(1): 71-81.
[14] Merkel R C, Toerien C, Sahlu T, et al . Digestibility, N balance and blood metabolite levels in Alpine goat wethers fed either water oak or shining sumac leaves. Small Ruminant Research the Journal of the International Goat Association, 2001, 40(2): 123-127.
[15] Diao Q Y. The latest research dynamic of tannins. Feed Research, 1999, (11): 28.
刁其玉. 单宁的最新研究动态. 饲料研究, 1999, (11): 28.
[16] Yu F, Barry T N, Mcnabb W C, et al . Effect of bound condensed tannin from cottonseed upon in situ, protein solubility and dry matter digestion in the rumen. Journal of the Science of Food and Agriculture, 1995, 69(3): 311-319.
[17] Robbins C T, Hanley T A, Hagerman A E, et al . Role of tannins in defending plants against ruminants: reduction in protein availability. Ecology, 1987, 68(1): 98-107.
[18] Silanikove N, Perevolotsky A, Provenza F D. Use of tannin-binding chemicals to assay for tannins and their negative postingestive effects in ruminants. Animal Feed Science and Technology, 2001, 91(1/2): 69-81.
[19] Cortés J E, Moreno B, Pabón M L, et al . Effects of purified condensed tannins extracted from Calliandra, Flemingia and Leucaena on ruminal and postruminal degradation of soybean meal as estimated in vitro . Animal Feed Science and Technology, 2009, 151(3): 194-204.
[20] Ngamsaeng A, Wanapat M, Khampa S. Evaluation of local tropical plants by in vitro rumen fermentation and their effects on fermentation end-products. Pakistan Journal of Nutrition, 2006, 5(5): 717-719.
[21] Calixto J B, Nicolau M, Rae G A. Pharmacological actions of tannic acid. I. Effects on isolated smooth and cardiac muscles and on blood pressure. Planta Medica, 1986, 52(1): 32.
[22] Neto S G, Batista A M V, de Carvalho F F R, et al . Bromatological composition, intake and in vivo digestibility of the diets with different levels of “catingueira” hay ( Caesalpinea bracteosa ), Fed to Morada Nova Sheep. Revista Brasileira De Zootecnia, 2001, 30(2): 553-562.
[23] Luque A, Barry T N, Mcnabb W C, et al . The effect of grazing Lotus corniculatus during late summer-autumn on reproductive efficiency and wool production in ewes. Australian Journal of Agricultural Research, 2000, 51(3): 385-392.