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Acta Prataculturae Sinica ›› 2019, Vol. 28 ›› Issue (8): 61-71.DOI: 10.11686/cyxb2018244

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Effects on the storage quality of dry corn stalk of adding cabbage wastes and organic acid

REN Hai-wei1,2,3,4, SUN An-qi1, REN Jun-le1, LI Zhi-zhong1,*, FAN Wen-guang1, WANG Yu2,3,4, LIU Fei-fei1, SHEN Jia-li1, SUN Wen-bin1   

  1. 1.College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China;
    2.China Western Energy & Environment Research Center, Lanzhou University of Technology, Lanzhou 730050, China;
    3.Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou 730050, China;
    4.China Northwestern Collaborative Innovation Center of Low-carbon Urbanization Technologies, Lanzhou 730050, China
  • Received:2018-04-23 Online:2019-08-20 Published:2019-08-20
  • Contact: *,E-mail: zzli2004@lut.cn

Abstract: Based on the physicochemical complementarity between dry corn stalk (DCS) and cabbage waste (CW), this experiment investigated the effects of the addition of cabbage wastes on the storage quality of dry corn stalk. The effects of adding formic acid to DCS with CW were also tested. The microbial community diversity during storage was investigated using Miseq high throughput sequencing technology. Three materials were prepared for testing of their storage fermentation reactions: (i) DCS with no additives (SE); (ii) DCS mixed with CW (ME); (iii) DCS mixed with CW and with the addition of formic acid (FB). The prepared materials were stored at 18±1 ℃ for 60 days, to undergo a fermentation reaction similar to silage making. The dynamic changes in the chemical composition, fermentation quality and microbial community diversity were analyzed at 30-day intervals. It was found that dry matter loss and pH value of the ME and FB materials were significantly (P<0.05) lower than in the SE material, and that longer-term storage of 60 d was beneficial through reduction of acid detergent lignin (ADL) content. The microbial community data indicated a greater diversity and abundance of lactic acid bacteria in ME and FB than in SE materials, with the more important genera identified being Lactobacillus, Paralactobacillus, Carnobacterium, Lactococcus, Pediococcus and Enterococcus. The main spoilage bacterium identified during storage was Enterobacter, and the abundance of Enterobacter in FB was significantly lower than in SE material. In summary, addition of CW to DCS reduced the loss of dry matter during fermentation and storage, and improved the structure of the microbial community. The application of formic acid during mixed storage of DCA and CW can further assist lignocellulosic breakdown.

Key words: dry corn stalk (DCS), cabbage wastes (CW), formic acid, storage quality