Effects of environmental factors on population genetic diversity of Tamarix chinensis

doi:10.11686/cyxb2018691

Abstract

Abstract: In this study, we examined the relationships between environmental factors and the genetic diversity of 20 Tamarix chinensis. populations throughout its native range. Genetic diversity was evaluated by comparing the sequences of nuclear ribosomal internal transcribed spacer regions (ITS) and two chloroplast DNA (cpDNA) regions (trnL-trnF and rps16). Eleven haplotypes were identified on the basis of 10 variable sites within the 616 bp sequenced ITS region, and the total nucleotide diversity and haplotype diversity indexes were 2.170 and 0.814, respectively. Sixteen haplotypes were identified on the basis of 14 variable sites within the two chloroplast regions (1542 bp), and the total nucleotide diversity and haplotype diversity indexes were 0.500 and 0.586, respectively. The results showed that the genetic diversity based on the ITS region is influenced mainly by altitude, temperature, and longitude. Populations with rich genetic diversity exist at lower altitudes, in warmer habitats, and in eastern regions nearer to the sea. We detected no significant relationships between the genetic diversity of cpDNA and environmental variables. Using the Mantel test, a significant relationship was detected between matrices of ITS genetic differentiation and matrices of geographical distance, but this relationship was not detected from the cpDNA data. This result further proved that seed flow is an important factor contributing to the low genetic differentiation of T. chinensis populations.

Key words: Tamarix chinensis, nucleotide diversity, haplotype diversity, genetic differentiation, environmental factors

SUN Li-kun, LIU Guang-xiu, ZHANG Bao-gui, ZHANG Gao-sen. Effects of environmental factors on population genetic diversity of Tamarix chinensis[J]. Acta Prataculturae Sinica, 2019, 28(10): 178-186.

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