Physiological aspects of insect resistance of Achnatherum inebrians after spraying with methyl jasmonate or with Epichloё gansuensis infection

doi:10.11686/cyxb2019289

Abstract

Abstract: The grass Achnatherum inebrians forms a symbiotic relationship with Epichloё gansuensis endophyte, which can promote plant growth and improve abiotic and biotic stress tolerance. The experiment was carried out in a greenhouse on the Yuzhong campus of Lanzhou University [Temperature (25±2) ℃; Humidity: 37.7%-48.3%], using endophyte-infected (E+) and nil-endophyte (E-) seedlings of A. inebrians from the Yuzhong region of Gansu province. After planting, seedings were exposed to exogenous methyl jasmonate (MJ) at concentrations of 200 or 400 mg·L^-1 to simulate herbivore feeding, and the physiological response assessed. It was found that, compared with the control, MJ treatments significantly decreased the soluble sugar content, and chlorophyll content (P<0.05) but significantly increased total phenol content (P<0.05). Effects of MJ treatment on herbivore resistance were different between E+ and E- plants. For E- plants, MJ treatment significantly increased polyphenoloxidase activity and phenylalanine ammonia-lyase activity. For E+ plants, the activity of trypsin inhibitors was significantly increased compared with the control group (P<0.05). In conclusion, MJ treatment strengthened the defensive ability of the host plant at the cost of reduced palatability, thus inhibiting the feeding of herbivorous insects. MJ treatment did enhance A. inebrians-mediated insect resistance.

Key words: methyl jasmonate, Epichloё endophyte, drunken horse grass, insect resistance

HE Ya-li, CHEN Zhen-jiang, WEI Xue-kai, ZHANG Hai-juan, LIU Yang, LIU Hui, LI Chun-jie. Physiological aspects of insect resistance of Achnatherum inebrians after spraying with methyl jasmonate or with Epichloё gansuensis infection[J]. Acta Prataculturae Sinica, 2020, 29(3): 121-129.

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