长期饲喂高精料日粮对泌乳期山羊血液生化参数及组织中糖代谢相关基因表达的影响

doi:10.11686/cyxb20140535

摘要/Abstract

摘要：

为了研究长期饲喂高精料日粮对泌乳期山羊血液生化指标和糖代谢的影响,选择8只安装永久性瘤胃瘘管的健康经产泌乳中期山羊,分别饲喂精粗比为40∶60 (low concentrate, LC,n=4) 和60∶40 (high concentrate, HC,n=4)的日粮。实验期共8周,实验期间采集瘤胃液,血液;实验结束时采集肝脏,肌肉,脂肪组织置于液氮速冻后于-70℃保存待测。结果显示,饲喂高精料日粮2周后山羊瘤胃液pH显著降低,采食后瘤胃液pH持续低于5.8超过3 h,8周后瘤胃液pH升高维持在6.0以上,但仍低于LC组山羊。与LC组山羊相比较,HC组山羊血液中葡萄糖、游离脂肪酸含量均升高(P<0.05,P=0.071),谷草转氨酶含量有上升的趋势(0.05<P<0.1);HC组肝脏中葡萄糖转运蛋白4 (Glut4) mRNA 表达有上升的趋势(P=0.06),脂肪和肌肉中丙酮酸羧化酶(PC)、线粒体型磷酸烯醇式丙酮酸羧激酶(PCK2)均呈上升趋势(P=0.066,P=0.069)。长期饲喂高精料日粮引起泌乳山羊能量代谢异常,并伴随血液代谢异常及组织糖代谢基因表达的变化。

Abstract:

In order to investigate the effect of feeding high concentrate diets for a long-term on plasma biochemical parameters and expression of genes involved in glucose metabolism in different tissues, 8 healthy multiparous goats in mid-lactation were rumen-cannulated in and were randomly allocated to two groups, one receiving a diet with low concentrate (LC, concentrate∶roughage, %=40∶60) as the LC group (n=4), and another receiving high concentrate diet (HC, concentrate∶roughage, %=60∶40) as the HC group (n=4). The experiment lasted for 8 weeks. At the end of experiment, tissues including liver, muscle and fat were sampled and immediately immersed in liquid nitrogen and then stored at -70℃ for later gene expression analysis. After feeding the high-concentrate diet for 2 weeks, the mean ruminal pH value of HC group had declined to less than 5.8, with low pH persisting 3 hours after feeding. However, after being fed the high concentrate diet for 8 weeks, the HC goats showed an increase in ruminal pH (mean pH>6.0), although this value was still significantly lower than that of LC goats (P<0.05). Among the biochemical parameters detected in plasma, the level of glutamic oxalacetic transaminase (AST) showed a tendency to increase (P=0.09), and the level of glucose (P<0.05) and non-esterified fatty acid (NEFA, P=0.071) was increased in HC goats compare to control. The gene expression of Glut4, pyruvate carboxylase (PC) and mitochondrial type phosphoenolpyruvate carboxylase (PCK2) in liver, fat and muscle, respectively, showed a tendency to increase (P=0.06, P=0.066 and P=0.069, respectively). These results suggest that prolonged feeding of a high concentrate diet increased plasma NEFA, glucose and AST, and up-regulated gene expression involved in glucose metabolism.

中图分类号:

S827.4⁺2

董海波,王绍庆,贾媛媛,端木永前,陶诗煜,倪迎冬,张源淑,庄苏,沈向真,赵茹茜. 长期饲喂高精料日粮对泌乳期山羊血液生化参数及组织中糖代谢相关基因表达的影响[J]. 草业学报, 2014, 23(5): 295-302.

DONG Hai-bo,WANG Shao-qing,JIA Yuan-yuan,DUANMU Yong-qian,TAO Shi-yu,NI Ying-dong,ZHANG Yuan-shu,ZHUANG Su,SHEN Xiang-zhen,ZHAO Ru-qian. Effect of long-term feeding of high concentrate diet on plasma biochemical parameters and expression of genes involved in glucose metabolism in lactating goats[J]. Acta Prataculturae Sinica, 2014, 23(5): 295-302.

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