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草业学报 ›› 2015, Vol. 24 ›› Issue (6): 159-167.DOI: 10.11686/cyxb2014274

• 研究论文 • 上一篇    下一篇

种荚预处理对红芪种子发芽特性的影响

孙云波1, 4, 陈翔2, 陈垣1*, *, 周传猛1, 白德涛3   

  1. 1.甘肃农业大学,甘肃省作物遗传改良与种质创新重点实验室,甘肃省中药材规范化生产技术创新重点实验室,农学院,甘肃 兰州 730070;
    2.中国科学院寒区旱区环境与工程研究所,沙漠与沙漠化重点实验室,甘肃 兰州 730000;
    3.陇西中天药业有限责任公司,甘肃省特色药用植物资源保护与利用工程实验室,甘肃省特色药材规范化可追溯栽培工程技术研究中心,甘肃 定西 748100;
    4.中国医学科学院药用植物研究所,北京 100193
  • 收稿日期:2014-06-09 出版日期:2015-06-20 发布日期:2015-06-20
  • 通讯作者: 2012年甘肃省战略性新兴产业创新支撑工程专项,甘肃省中药材扶持项目(151402),全国第四次中药资源普查甘肃(试点)岷县,宕昌县中药资源普查(201207002-02和2013GSZYZYPC-02)和甘肃农业大学教学研究项目资助
  • 作者简介:孙云波(1985-),女,吉林临江人,在读硕士。E-mail: syb_0229@163.com

Effects of pod pre-treatments on the germination traits of Hedysarum polybotrys seeds

SUN Yun-Bo1, 4, CHEN Xiang2, CHEN Yuan1, *, ZHOU Chuan-Meng1, BAI De-Tao3   

  1. 1.Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Provincial Key Laboratory of Good Agricultural Productive Technology for Traditional Chinese Medicines, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
    2. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
    3.Gansu Engineering Laboratory of Resource Reservation and Utilization for Characteristic Medical Plants, Gansu Cultivated Engineering and Technology Research Center of Standardization and Traceability for Characteristic Chinese Medicine, Longxi Zhongtian Pharmaceutical Co., Ltd, Dingxi 748100, China;
    4.The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
  • Received:2014-06-09 Online:2015-06-20 Published:2015-06-20

摘要: 采用去除果荚浸种、不去除果荚浸种荚和焖种荚等方法对红芪种子预处理12 h后再行发芽试验,以不去果荚不浸种干种荚为对照,旨在探寻改善红芪种子发芽特性的种子预处理方法。结果表明,春播季节20℃/18℃自然光照条件下,各处理红芪种子发芽率随发芽天数的动态变化均呈“S型”变化趋势,符合Logistic曲线方程。红芪种荚从置发芽床后第2~6天开始,种子先后启动发芽,第14~19天发芽陆续结束。整个发芽过程中,种子各发芽指标均表现出预处理间的显著差异性,预处理使红芪种子发芽提早1~4 d,发芽高峰持续期延长2 d。去果荚浸种12 h显著加快了种子发芽进程,焖种荚处理有效延长了发芽持续期。各处理发芽指标从高到低依次为去果荚浸种12 h(发芽率80.7%,发芽势54.0%,发芽指数7.083)>不去果荚焖种荚12 h(发芽率64.0%,发芽势13.3%,发芽指数3.462)>不去果荚水浸种荚12 h(发芽率48.0%,发芽势22.0%,发芽指数3.007)>对照干种荚(发芽率34.7%,发芽势4.0%,发芽指数1.865)。以上说明种荚果皮造成的吸水障碍是限制红芪种子发芽的主要因素,播种前将红芪种荚浸种12 h或焖种12 h再行播种均可显著改善红芪种子发芽特性,去除果荚后再浸种效果更佳,发芽率可达80.0%以上。

Abstract: Plant pod seeds of Hedysarum polybotrys were used in a germination test in order to explore the effect of seed pre-treatment methods. Several methods, such as soaking the peeled seeds, and soaking and stewing the unpeeled seed pods for 12 h, were compared with non-soaked, dry seed pods as a control. Results under natural illumination at 18-20℃ showed that germination dynamics in all pre-treatments followed S curve patterns that fitted to a logistic equation. Germination started days 2-6 and finished days 14-19. The pre-treatments showed significant differences in germination traits. The peeled and soaking pre-treatment and the soaking and stewed unpeeled-seed pods pre-treatment delayed peak germination by two days. The peeled and soaking pre-treatment significantly expedited germination while the unpeeled and stewing pre-treatment prolonged germination duration. All the pre-treatments for 12 h significantly improved germination quality compared with the control. The control had a germination rate of 34.7%, germination power 4.0% and germination index 1.865. The results for the peeled and soaked seeds were 80.7%, 54.0% and 7.083 respectively, that for the unpeeled stewed seed pods 64.0%, 13.3% and 3.462 respectively. The results thus show that pre-treating seeds by soaking or stewing can significantly improve germination quality. Improvements were highest in the peeled pre-treatment, where seeds showed over 80% germination rate.