不同放牧强度下垂穗披碱草遗传多样性分析

doi:10.11686/cyxb2014355

草业学报 ›› 2015, Vol. 24 ›› Issue (8): 159-165.DOI: 10.11686/cyxb2014355

不同放牧强度下垂穗披碱草遗传多样性分析

陈钊¹, 梁新平¹, 侯扶江², 田苗苗¹, 张红瑞¹, 余莹¹, 管永卓¹, 王成章¹, 严学兵^{1*, *}

1.河南农业大学牧医工程学院,河南郑州 450002;
2.兰州大学草地农业科技学院,草地农业生态系统国家重点实验室,甘肃兰州 730020

出版日期:2015-08-20 发布日期:2015-08-20
通讯作者: E-mail:yxbbjzz@163.com
作者简介:陈钊(1990-),男,河南滑县人,硕士。E-mail:740515547@qq.com
基金资助:
国家自然科学基金项目(30901051,31172249)资助

Genetic diversity of Elymus nutans under different grazing intensities

CHEN Zhao¹, LIANG Xin-Ping¹, HOU Fu-Jiang², TIAN Miao-Miao¹, ZHANG Hong-Rui¹, YU Ying¹, GUAN Yong-Zhuo¹, WANG Cheng-Zhang¹, YAN Xue-Bing^{1, *}

1.College of Animal &Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China;
2.College of Pastoral Agriculture Science and Technology, Lanzhou University, State Key Laboratory of Grassland Agro-ecosystems, Lanzhou 730020, China

Online:2015-08-20 Published:2015-08-20

摘要/Abstract

摘要： 放牧对草地植物种群遗传与进化产生重要影响,本研究利用SSR分子标记对4个不同放牧强度下垂穗披碱草种群遗传多样性进行研究,试验地选择在甘肃省甘南自治州玛曲县的阿孜试验站,利用8对多态性强的SSR引物对不同放牧压力下4个居群的800个个体基因组进行检测,每个位点的有效等位基因数在1.2267~1.9976之间。利用popgene分析发现不放牧垂穗披碱草种群遗传多样性最高,在3种不同放牧地,中等放牧强度的遗传多样性指数较高,其次为重牧,最后为轻度放牧。在不同放牧干扰下的4个垂穗披碱草种群的遗传分化系数为0.5168,基因流N_m=0.2337,说明4个种群的遗传变异主要发生在种群之间。从种质资源保护角度来讲,不放牧对于垂穗披碱草种质资源的保护是有利的;从草地利用角度,中等放牧强度比较合理。

Abstract: Grazing can influence the population genetics and evolution of grassland plant species. To study the relationship between grazing and the potential for evolutionary differentiation and gene flow, we used SSR markers to study the genetic diversity of Elymus nutans. The experiment station investigated is based at Azi, in Maqu County in the Gannan region of Gansu Province. The grazing lands were divided into four levels according to different grazing intensities. Eight pairs of SSR primers were used to detect genetic diversity among 800 individual plants from the four populations under different grazing pressures. The effective number of alleles per locus ranged from 1.2267 to 1.9976. We found that materials under moderate grazing intensity had the highest genetic diversity index, followed by the heavy and then light grazing levels. The genetic differentiation coefficient under different grazing levels is 0.5168. This suggests that genetic variation of the four populations exists mainly among populations. In conclusion, no grazing or enclosure is effective for the conservation of E. nutans genetic resources. For grassland utilization, however, moderate grazing is relatively optimal.

陈钊, 梁新平, 侯扶江, 田苗苗, 张红瑞, 余莹, 管永卓, 王成章, 严学兵. 不同放牧强度下垂穗披碱草遗传多样性分析[J]. 草业学报, 2015, 24(8): 159-165.

CHEN Zhao, LIANG Xin-Ping, HOU Fu-Jiang, TIAN Miao-Miao, ZHANG Hong-Rui, YU Ying, GUAN Yong-Zhuo, WANG Cheng-Zhang, YAN Xue-Bing. Genetic diversity of Elymus nutans under different grazing intensities[J]. Acta Prataculturae Sinica, 2015, 24(8): 159-165.

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不同放牧强度下垂穗披碱草遗传多样性分析

Genetic diversity of Elymus nutans under different grazing intensities

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