[1] Chen B S. Research of yield and nutrition in sainfoin. Grassland of China, 1983, (2): 36-38. [2] Chen B S. Sainfoin is an excellent leguminous forage and a plant for water and soil conservation. Science of Soil and Water Conservation, 1984, 29(8): 14-18. [3] Chen B S. Sainfoin[M]. Lanzhou: Gansu Science and Technology Press, 1992: 18. [4] Howarth R E, Sarkar S K, Fesser A C, et al . Some properties of soluble proteins from alfalfa ( Medicago sativa ) herbage and their possible relation to ruminant bloat. Journal of Agricultural and Food Chemistry, 1977, 25(1): 175-179. [5] Wu Z L, Niu J L, Zhang Y P, et al . Tannin dynamics study for sainfoin. Chinese Journal of Grassland, 1993, (5): 25-27. [6] McMahon L R, McAllister T A, Berg B, et al . A review of the effects of forage condensed tannins on ruminal fermentation and bloat in grazing cattle. Canadian Journal of Plant Science, 2000, 80: 469-485. [7] Wang Y P, Yang Z M, Zhang B. Identification of protein related to biosynthesis of condensed Tannin in Onobrychis tanaitica spreng. Acta Agrestia Sinica, 2000, 8(2): 126-131. [8] Zhao W J, Zhang D, Ma L J, et al . Biosynthetic pathway, functional genes and metabolic engineering of proanthocyanidins. Plant Physiology Communications, 2009, 45(5): 509-519. [9] Xie D Y, Sharma S B, Paiva N L, et al . Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis. Science, 2003, 299: 396-399. [10] Bogs J, Downey M O, Harvey J S, et al . Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves. Plant Physiology, 2005, 139(2): 652-663. [11] Yuan L, Wang L, Han Z, et al . Molecular cloning and characterization of PtrLAR3, a gene encoding leucoanthocyanidin reductase from Populus trichocarpa , and its constitutive expression enhances fungal resistance in transgenic plants. Journal of Experiment Botany, 2012, 63(7): 2513-2524. [12] Paolocci F, Robbins M P, Madeo L, et al . Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosyn thesis: structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus. Plant Physiology, 2007, 143(1): 504-516. [13] Wang Y, Zhang Q L, Luo Z R. Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development. Biologia Plantarum, 2010, 54(4): 707-710. [14] Pang Y, Wenger J P, Saathoff K, et al . A WD40 repeat protein from Medicago truncatula is necessary for tissue-specific anthocyanin and proanthocyanidin biosynthesis but not for trichome development. Plant Physiology, 2009,151(3): 1114-1129. [15] Sambrook J, Russell D W. Molecular Cloning: A Laboratory Manual[M]. Vol.2. Beijing: Beijing Sciences Press, 1995: 343-392. [16] Shi B S, Zhuo L H. Modification and comparation of total extraction methods from Prunus cerasifera leaves. Molecular Plant Breeding, 2006, 4(5): 721-725. [17] Terrill T H, Rowan A M, Douglas G B. et al . Determination of extractable and bound condensed tannin concentrations in forage plants, prote in concentrate meals and cereal grains. Journal of the Science of Food and Agriculture, 1992, 58(3): 321-329. [18] Li H, Wang X L. The difficulties in the isloation of RNA from plant tissues and their resolving strategies. Bio Technology Information, 1999, (1): 36-39. [19] Gasic K, Hernandez A, Korban S S. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Molecular Biology Reporter, 2004, 22(4): 437-438. [20] Rodrigues S M, Soares V L F, de Oliveira T M, et al . Isolation and purification of RNA from tissues rich in polyphenols, polysaccharides, and pigments of annatto ( Bixa Orellana L.). Molecular Biotechnology, 2007, 37(3): 220-224. [21] Ainsworth C. Isolation of RNA from floral tissue of Rumex acetosa (sorrel). Plant Molecular Biology Reporter, 1994, 12(3): 198-203. [22] Tanner G J, Francki K T, Abrahams S, et al . Proanthocyanidin biosynthesis in plants:purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA. Journal of Biology Chemistry, 2003, 278: 31647-31656. [23] Ma J, Wang B, Dai Y, et al . Cloning and expression analysis of leucoanthocyanidin reductase gene in Fagopyrum dibotrys . Acta Pharmaceutica Sinica, 2012, 47(7): 953-961. [24] Thill J, Regos I, Farag M A, et al . Polyphenol metabolism provides a screening tool for beneficial effects of Onobrychis viciifolia (sainfoin). Phytochemistry, 2012, 82: 67-80. [25] Shen B Y, Liu Y H, Zhang J L, et al . Analysis of cloning and sequence characteristics of cDNA ending the GBSS I gene from tubers of Sloanum tuberosum . Acta Prataculturae Sinica, 2010, 19(5): 1-8. [26] Li L Q, Huang Y B, Wang X Y. Cloning, sequence analysis and prokaryotic expression of the WRKY6 gene of potato. Acta Prataculturae Sinica, 2011, 20(2): 177-183. [27] Lv H Y, Li Y X, Kong F J, et al . Recent progress in studies on Na + /H + antiporter in plants. Chinese Bulletin of Botany, 2003, 20(3): 363-369. [28] Wang L, Jiang Y, Yuan L, et al .Isolation and characterization of cDNAs encoding leucoanthocyanidin reductase and anthocyanidin reductase from Populus trichocarpa . PLoS One, 2013, 8(5): e64664. doi:10.1371/journal. Pone. 0064664. [29] Daniel A H. Biomass allocation is an important determinant of the tannin concentration in growing plants. Annals of Botany, 2007, 99: 111-120. [1] 陈宝书. 红豆草产量和营养成分的研究. 中国草原, 1983, (2): 36-38. [2] 陈宝书. 红豆草是优良的豆科牧草和水土保持植物. 中国水土保持, 1984, 29(8): 14-18. [3] 陈宝书. 红豆草[M]. 兰州: 甘肃科学技术出版社, 1992: 18. [5] 吴自立, 牛菊兰, 张跃平, 等. 红豆草中丹宁的动态研究. 中国草地学报, 1993, (5): 25-27. [7] 王玉萍, 杨茁萌, 张博. 顿河红豆草缩合单宁生物合成相关蛋白鉴定. 草地学报, 2000, 8(2): 126-131. [8] 赵文军, 张迪, 马丽娟, 等. 原花青素的生物合成途径、功能基因和代谢工程. 植物生理学通讯, 2009, 45(5): 509-519. [15] 萨姆布鲁克J, 拉塞尔D W. 分子克隆实验指南[M]. 2版. 北京: 科学出版社, 1995: 343-392. [16] 史宝胜, 卓丽环. 紫叶李叶片总RNA提取方法的改进与比较. 分子植物育种, 2006, 4(5): 721-725. [18] 李宏, 王新力. 植物组织RNA提取的难点及对策. 生物技术通报, 1999, (1): 36-39. [23] 马婧, 王斌, 代银, 等. 金荞麦无色花色素还原酶基因FdLAR的克隆和表达分析. 药学学报, 2012, 47(7): 953-961. [25] 沈宝云, 刘玉汇, 张俊莲, 等. 马铃薯块茎GBSSI基因的cDNA克隆及其序列特征分析. 草业学报, 2010, 19(5): 1-8. [26] 李立芹, 黄玉碧, 王西瑶. 马铃薯WRKY6基因的克隆、序列分析与原核表达研究. 草业学报, 2011, 20(2): 177-183. [27] 吕慧颖, 李银心, 孔凡江, 等. 植物Na + /H + 逆向转运蛋白研究进展. 植物学通报, 2003, 20(3): 363-369. |