紫花苜蓿根腐病原菌——拟枝孢镰刀菌的鉴定及其生物学特性研究

doi:10.11686/cyxb2014466

草业学报 ›› 2015, Vol. 24 ›› Issue (10): 88-98.DOI: 10.11686/cyxb2014466

紫花苜蓿根腐病原菌——拟枝孢镰刀菌的鉴定及其生物学特性研究

潘龙其¹, 张丽¹, 杨成德^{1*, *}, 袁庆华^{2*, *}, 王瑜², 苗丽宏²

1.甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070;
2.中国农业科学研北京畜牧兽医研究所,北京100193

出版日期:2015-10-20 发布日期:2015-10-20
通讯作者: *通讯作者Corresponding author. E-mail:yangcd@gsau.edu.cn, yuanqinghua@iascaas.net.cn
作者简介:潘龙其(1988-),男,甘肃天水人,在读硕士。E-mail:gsaupanlongqi@126.com
基金资助:
公益性行业(农业)科研专项项目(201303057)和国家科技支撑项目“优质多抗牧草新品种选育与良种繁育关键技术研究与示范”(2011BAN17B01)资助

Identification and biological characteristics of Fusarium sporotrichioide isolated from Medicago sativa root

PAN Long-Qi¹, ZHANG Li¹, YANG Cheng-De^{1, *}, YUAN Qing-Hua^{2, *}, WANG Yu², MIAO Li-Hong²

1.College of Grassland, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
2.Institute of Animal Sciences of CAAS, Beijing 100193, China

Online:2015-10-20 Published:2015-10-20

摘要/Abstract

摘要： 本文对从内蒙古赤峰巴雅尔草业基地紫花苜蓿根部分离到的菌株BYE27-2-5进行了致病性测定、形态学和rDNA-ITS序列分析鉴定及生物学特性测定。结果表明,该菌菌丝生长、产孢和孢子萌发的最适温度分别为25,30和28℃;pH值5.0~11.0均宜于菌丝生长,产孢最适pH 8.0,孢子萌发最适pH 7.0;光暗交替利于菌丝生长,光照利于产孢和孢子的萌发;孢子在相对湿度低于75%不萌发,高于95%萌发较快;葡萄糖蛋白胨培养基利于菌丝生长和孢子萌发,马铃薯蔗糖琼脂培养基利于产孢;碳源中葡萄糖利于菌丝生长和孢子萌发,蔗糖利于产孢;氮源中蛋白胨利于菌丝生长,酵母膏利于产孢和孢子萌发;菌丝致死温度为54℃(10 min),分生孢子的致死温度为48℃(10 min);根据其形态特征和rDNA-ITS序列分析结果,鉴定其为拟枝孢镰刀菌(Fusarium sporotrichioide)。

Abstract: The strain BYE27-2-5 isolated from alfalfa (Medicago sativa) with root rot in north China was comprehensively evaluated by analyses of its biological characteristics, morphology, and ITS sequence. The optimum temperatures for mycelium growth, sporulation, and spore germination were 25℃, 30℃, and 28℃, respectively. The strain was able to grow in a pH range of 5.0-11.0 and the optimal pH for sporulation and spore germination were 8.0 and 7.0, respectively. Alternative light conditions were optimal for mycelial growth whereas complete light conditions were optimal for sporulation and spore germination. Spores germinated most rapidly at 95% relative humidity or in water-drop conditions, and failed germinate at relative humidity levels below 75%. Dextrose peptone medium was the best medium for mycelial growth and spore germination and potato dextrose agar was the best medium for sporulation. The best carbon source for mycelial growth and spore germination was glucose and that for sporulation was sucrose. The best nitrogen source for mycelium growth was peptone and that for spore germination and sporulation was yeast extract. The lethal temperature for mycelia was 54℃ (10 min) and that for spores was 48℃ (10 min). Based on these biological characteristics and the results of an ITS sequence analysis, the isolate was identified as Fusarium sporotrichioide.

潘龙其, 张丽, 杨成德, 袁庆华, 王瑜, 苗丽宏. 紫花苜蓿根腐病原菌——拟枝孢镰刀菌的鉴定及其生物学特性研究[J]. 草业学报, 2015, 24(10): 88-98.

PAN Long-Qi, ZHANG Li, YANG Cheng-De, YUAN Qing-Hua, WANG Yu, MIAO Li-Hong. Identification and biological characteristics of Fusarium sporotrichioide isolated from Medicago sativa root[J]. Acta Prataculturae Sinica, 2015, 24(10): 88-98.

参考文献

[1] Nan L L, Shi S L, Zhang J H. Study on root system development ability of different root-type alfalfa. Acta Prataculturae Sinica, 2014, 23(2): 117-124.
[2] Tian C X, Zhang Y M, Wang K, et al . The anatomical structure responses in alfalfa tosalinity-alkalinity stress of NaHCO 3 . Acta Prataculturae Sinica, 2014, 23(5): 133-142.
[3] Huang N, Lu X S. Research and resistance evaluation progress on alfalfa leaf and root diseases.Chinese Agricultural Science Bulletin, 2012,28(5):1-7.
[4] Liu H B, Yu Y X. Research progress in root rot of alfalfa. Grassland and Turf (Bimonthly), 2006, 3: 3-13.
[5] Zhang F C. The control measure of alfalfa common diseases and insect pests. Journal of Sichuan Grassland,1994, (1): 59-64.
[6] Wang X W, Wang C L, Zhou G, et al . Investigation and analysis on lucerne diseases from altay newly-established areas in Northern Xinjiang. Journal of Xinjiang Agricultural University,1996, 19(3): 40-44.
[7] Chen Y, He J C, Xiao F, et al . The preliminary study on root rot diseases of alfalfa in Xinjiang uygur autonomous region.Grassland of China, 1989, (2): 71-73.
[8] Hu Q Q. The occurring status and control measure of alfalfa root rot. Inner Mongolia Prataculture, 2009,21(1):37-40.
[9] Cao L X, Zhao C H, Kong Q Q, et al . Research progress of control and pathogen in alfalfa root rot. Inner Mongolia Agricultural Science And Technology, 2006, (3):36-37.
[10] Li M Q, Chai Z X, Li J H, et al . Identification pathogens of crown and root rot of alfalfa in Dingxi region. Acta Agrestia Sinica, 2003, 11(1):83-86.
[11] Chen Y J, Liu X M, Cui G W, et al . Research progress in root rot of alfalfa.Acta Agrestia Sinica, 2000, (1):51-56.
[12] Liang Q W, Sun D X, Wu Y H, et al . The problems and suggestion of alfalfa in Chifeng. Feed Research, 2014, 3: 68-70.
[13] Fang Z D. The Method on Studies of Plant Pathology[M]. Beijing:Chinese Agricultural Press, 1998: 124.
[14] Qiu X Y, Tang Z P, Zhang M, et al . Research on the isolation method of single spore of most plant pathogenic fungi. Journal of Anhui Agriculture Science, 2011, 39(9): 5263-5264.
[15] Booth C. The Genus Fusarium [M]. Chen Q Y. Beijing: Agricultural Press, 1988: 103.
[16] Zhang Y, Zheng J Q, Xie B Y, et al . Identification on pathogen of cabbagewilt disease. Acta Phytopathologica Sinica, 2008, 38(4): 337-345.
[17] Liu Z H, Zheng C, Huang X Y, et al . Pathogen identification and biological characteristics of eggplant leaf mold. Plant Protection, 2013, 43(3): 58-69.
[18] Wang G C, Zheng Z, Ye Q M, et al . Guide to the Identification of Common Fusarium[M]. Beijing: Chinese Agricultural Sciences and Technology Press, 1996: 36-37.
[19] Xiao J W, Ran J X, Yang Z C, et al . Identification of Fusarium isolates from America soybean. Plant Quarantine, 2011, (1): 29-32.
[20] Du B, Yang S C, Liu C Q, et al . The pathogen of erigeron breviscapus root rot in Yunnan.Scientia Agricultura Sinica, 2007, 40(8): 1830-1834.
[21] Wang D C, Meng Y R, Li W M, et al . Isolation and identification of the pathogens causing root rot disease of Medicago sativa . Pratacultural Science, 2005, 22(10): 78-81.
[22] Li W C, Li W M, Meng Y R. Biological characteristics of dry root rot ( Fusarium solani ) of Medicago sativa . Acta Prataculturae Sinica, 2005, 14(4): 106-111.
[1] 南丽丽, 师尚礼, 张建华. 不同根型苜蓿根系发育能力研究. 草业学报, 2014, 23(2): 117-124.
[2] 田成霞, 张咏梅, 王凯, 等. 紫花苜蓿组织解剖结构对NaHCO 3 盐碱胁迫的响应. 草业学报, 2014, 23(5): 133-142.
[3] 黄宁, 卢欣石. 苜蓿叶部与根部病害研究的评价进展.中国农学通报, 2012, 28(5):1-7.
[4] 刘海波, 玉永雄. 紫花苜蓿根腐病研究进展. 草原与草坪, 2006, 3: 3-13.
[5] 张富川. 苜蓿常见病虫害的防治措施. 四川草原, 1994, (1): 59-64.
[6] 王雪薇, 王纯利, 周刚, 等. 新疆阿勒泰新垦区苜蓿病害调查与分析. 新疆农业大学学报, 1996,19(3): 40-44.
[7] 陈耀, 阂继淳, 肖凤, 等. 新疆苜蓿根腐病研究初报. 中国草地, 1989, (2): 71-73.
[8] 胡清泉. 紫花苜蓿根腐病的发生现状与防治措施. 内蒙古草业, 2009,21(1):37-40.
[9] 曹丽霞, 赵存虎, 孔庆全, 等. 紫花苜蓿根腐病病原及防治研究进展. 内蒙古农业科技, 2006, (3):36-37.
[10] 李敏权, 柴兆祥, 李金花, 等. 定西地区苜蓿根和根颈腐烂病的研究. 草地学报, 2003, 11(1): 83-86.
[11] 陈雅君, 刘学敏, 崔国文, 等. 紫花苜蓿根腐病的研究进展. 中国草地, 2000, (1): 51-56.
[12] 梁庆伟, 孙德欣, 乌艳红, 等. 赤峰市苜蓿种植存在的问题与建议. 饲料研究, 2014, 3: 68-70.
[13] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998:124.
[14] 邱小燕, 汤志鹏, 张敏, 等. 一种适用于多数植物病原真菌的单孢分离方法. 安徽农业科学, 2011, 39(9): 5263-5264.
[15] 布斯.镰刀菌属[M]. 陈其瑛. 北京: 农业出版社, 1988:103.
[16] 张扬, 郑建秋, 谢炳炎, 等. 甘蓝枯萎病病原菌的鉴定. 植物病理学报, 2008, 38(4): 337-345.
[17] 刘志恒, 郑川, 黄欣阳, 等. 茄子绒菌病斑病原菌鉴定及生物学特性研究. 植物保护, 2013, 43(3): 58-69.
[18] 王拱辰, 郑重, 叶琪明, 等. 常见镰刀菌鉴定指南[M]. 北京:中国农业技术出版社, 1996: 36-37.
[19] 肖杰文, 冉俊祥, 杨占成, 等. 美国大豆中镰刀菌的分离鉴定. 植物检疫, 2011, (1): 29-32.
[20] 杜宾, 杨生超, 刘翠琼, 等. 云南灯盏花根腐病病原菌初步鉴定. 中国农业科学, 2007, 40(8): 1830-1834.
[21] 王多成, 孟有儒, 李文明, 等. 苜蓿根腐病病原菌的分离及鉴定. 草业科学, 2005, 22(10):78-81.
[22] 李万苍, 李文明, 孟有儒. 苜蓿根腐病菌( Fusarium solani )生物学特性研究. 草业学报, 2005, 14(4):106-111.

紫花苜蓿根腐病原菌——拟枝孢镰刀菌的鉴定及其生物学特性研究

Identification and biological characteristics of Fusarium sporotrichioide isolated from Medicago sativa root

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics