生物肥部分替代化肥对花椰菜产量、品质、光合特性及肥料利用率的影响

doi:10.11686/cyxb20150107

摘要/Abstract

摘要： 高原夏季蔬菜生产面临着化肥过量使用导致的蔬菜品质下降、肥料利用率低和地下水污染等问题,通过研究生物肥部分替代化肥,探讨生物肥的增效作用、替代量和肥料利用率,为菜田合理施肥与提高蔬菜品质提供科学依据。采用随机区组试验,设不施肥(CK)、100%常规施肥(100CF)、80%常规施肥(80CF)、60%常规施肥(60CF)、100%常规施肥+生物肥(100CFB)、80%常规施肥+生物肥(80CFB)、60%常规施肥+生物肥(60CFB)7个处理,研究了化肥配施生物肥对花椰菜产量、品质、养分分配、肥料利用率及光合特性的影响。结果表明,与单施化肥相比,配施生物肥可显著提高花椰菜花球单重,同时提高了氮、磷、钾肥利用率及其吸收量;与100%常规施肥相比,化肥减施20%配施生物肥提高了花球产量,而化肥减施40%配施生物肥显著降低了花球产量,化肥减量配施生物肥显著降低了花球硝酸盐含量,显著提高了花球维生素C和可溶性糖含量;以80CFB处理氮肥利用率和功能叶吸氮量最高,100CF处理花球吸氮量最高,易造成硝酸盐的积累;配施生物肥增加了叶片净光合速率和气孔导度,减小了叶片胞间CO₂浓度。因此,适当减施化肥配施生物肥(80%常规施肥+生物肥)在不影响花椰菜产量的同时,能够显著改善花椰菜的品质、提高肥料利用率和光合效率。

Abstract: The over-use of inorganic fertilizer in summer vegetable production leads to diminished vegetable quality, nutrient use inefficiency and groundwater pollution. It is not known whether the combined application of bio-fertilizer with mineral fertilizer can improve quality and yield. A field experiment with broccoli (Brassica oleracea var. botrytis) has been under taken to investigate the effects of different combinations on quality, yield, nitrogen, phosphorus and potassium distribution rates, fertilizer utilization rate and photosynthesis. Three different rates (60%, 80% and 100%) of the conventional doses of nitrogen, phosphorus and potassium fertilizers (CF) were combined with bio-fertilizer (CK) in 6 treatments as follows: 60% CF without bio-fertilizer (60CF), 60% CF with bio-fertilizer (60CFB), 80% CF without bio-fertilizer (80CF), 80% CF with bio-fertilizer (80CFB), 100% CF without bio-fertilizer (100CF) and 100% CF with bio-fertilizer (100CFB). These treatments were applied to the crop in a randomized complete block design with three replicates.The head weight of broccoli with bio-fertilizer treatments significantly increased compared to that of sole mineral fertilizer treatments. The bio-fertilizer treatments’ utilization rates and uptake of nitrogen, phosphorus and potassium were also higher. Compared with 100CF, the yield under 80CFB increased, whereas the yield under 60CFB decreased. Compared with 100CF treatment, the nitrate content in the broccoli head decreased significantly, while the vitamin C and soluble sugar contents significantly increased under lower CF and bio-fertilizer treatments. Nitrogen uptake was highest in the functional leaf of broccoli with 80CFB and in the head of broccoli with 100CF. Under the treatments with bio-fertilizer, the photosynthetic rate and stomatal conductance increased, whereas intercellular CO₂ leaf concentrations reduced. These results demonstrated that the 750 kg/ha bio-fertilizer combined with 80% conventional mineral fertilizer can improve broccoli head quality, fertilizer utilization rate and photosynthesis.

李杰, 贾豪语, 颉建明, 郁继华, 杨萍. 生物肥部分替代化肥对花椰菜产量、品质、光合特性及肥料利用率的影响[J]. 草业学报, 2015, 24(1): 47-55.

LI Jie, JIA Haoyu, XIE Jianming, YU Jihua, YANG Ping. Effects of partial substitution of mineral fertilizer by bio-fertilizer on yield, quality, photosynthesis and fertilizer utilization rate in broccoli[J]. Acta Prataculturae Sinica, 2015, 24(1): 47-55.

参考文献

[1] Selim E M, Mosa A A. Fertigation of humic substances improves yield and quality of broccoli and nutrient retention in a sandy soil. Journal of Soil Science and Plant Nutrition, 2012, 175: 273-281.
[2] Bai M J, Xu D, Zhang S H, et al . Spatial-temporal distribution characteristics of water-nitrogen and performance evaluation for basin irrigation with conventional fertilization and fertigation methods. Agricultural Water Management, 2013, 126: 75-84.
[3] Mahanta D, Bhattacharyya R, Gopinath K A, et al . Influence of farmyard manure application and mineral fertilization on yield sustainability, carbon sequestration potential and soil property of gardenpea-french bean cropping system in the Indian Himalayas. Scientia Horticulturae, 2013, 164: 414-427.
[4] Duan R, Tang Y F, Wen J, et al . Effect of reducing fertilizer application on crop yield and nitrogen and phosphorus loss in runoff from embankment upland in Dongting Lake Region. Chinese Journal of Eco-Agriculture, 2013, 21(5): 536-543.
[5] Meng L, Zhang X L, Jiang X F, et al . Effects of partial mineral nitrogen substitution by organic fertilizer nitrogen on the yields of rice grains and their proper substitution rate. Scientia Agricultura Sinica, 2009, 42(2):532-542.
[6] Cao D, Zong L G, Xiao J, et al . Effects of bio-fertilizer on organically cultured cucumber growth and soil biological characteristics. Chinese Journal of Applied Ecology, 2010, 21(10): 2587-2592.
[7] Marcotel I, Hernandez T, Garcia C, et al . Influence of one or two successive annual applications of organic fertilisers on the enzyme activity of a soil under barley cultivation. Bioresource Technology, 2011, 79: 147-154.
[8] Huang P, He T, Du J. Effect on water, fertilizer and light use efficiency of maize under biological bacterial fertilizer and chemical fertilizer reduction. Chinese Agricultural Science Bulletin, 2011, 27(3):76-79.
[9] Chang K H, Wu R Y, Chuang K C, et al . Effects of chemical and organic fertilizers on the growth, flower quality and nutrient uptake of Anthurium andreanum , cultivated for cut flower production. Scientia Horticulturae, 2010, 125: 434-441.
[10] Zhao Q L, Wu X, Yuan S J, et al . A study on the dynamics of phosphorus adsorption and desorption characteristics of paddy soil with long-term fertilization. Acta Prataculturae Sinica, 2014, 23(1):113-122.
[11] Miloševiĉ T, Miloševiĉ N, Glišiĉ I, et al . Fertilization effect on trees and fruits characteristics and leaf nutrient status of apricots which are grown at Cacak region (Serbia). Scientia Horticulturae, 2013, 164: 112-123.
[12] Luo S Q, Shi A D, Yuan L, et al . Changes in antimalarial compounds and antioxidation activities of Artemisia annua in response to fertilization. Acta Prataculturae Sinica, 2014, 23(1):339-345.
[13] Xu G W, Li S, Zhao Y F, et al . Effect of straw returning and nitrogen fertilizer application on root secretion and nitrogen utilization of rice. Acta Prataculturae Sinica, 2014, 23(2):140-146.
[14] Bao S D. Soil and Agrochemical Analysis[M]. Beijing: China Agriculture Press, 2000.
[15] He L Y, Liang H D. The research of HClO 4 -H 2 SO 4 digestion plants to prevent nitrogen loss. Chinese Journal of Analytical Chemistry, 1992, 20(11): 1277-1250.
[16] Zou Q. Guidance of Plant Physiology Experiments[M]. Beijing: China Agriculture Press, 2000.
[17] Yi Q, Zhang X Z, He P, et al . Effects of reducing N application on crop N uptake, utilization, and soil N balance in rice-wheat rotation system. Plant Nutrition and Fertilizer Science, 2010, 16(5):1069-1077.
[18] Liu Z F, Zhang G B, Yu J H, et al . Effects of different nitrogen forms and their ratios on broccoli yield, quality, and nutrient absorption. Chinese Journal of Applied Ecology, 2013, 24(7):1923-1930.
[19] Wang B Q, Yin N W, Zheng M H, et al . Effects of reduction of chemical fertilizer and organic manure supplement on vegetables yield and quality. Chinese Agricultural Science Bulletin, 2012, 28(1):242-247.
[20] Villeneuve S, Coulombe J, Bélec C, et al . A comparison of sap nitrate test and chlorophyll meter for nitrogen status diagnosis in broccoli ( Brassica oleracea L. var. italica ). Acta Horticulturae, 2002, 571: 171-177.
[21] Westerveld S M, McKeown A W, McDonald A R, et al . Chlorophyll and nitrate meters as nitrogen monitoring tools for selected vegetables in southern Ontario. Acta Horticulturae, 2003, 627: 259-266.
[22] Wang Q, Jiang L N, Fu J R, et al . Effects of form, rate and time of N fertilizer application on yield and nitrate content of greengrocery. Plant Nutrition and Fertilizer Science, 2008, 14(1): 126-131.
[23] Zhu L X, Bi J J, Wang J H, et al . Effects of mixture of controlled-release urea and conventional urea on the yield and nitrogen use efficiency of Chrysanthemum morifolium . Acta Horticulturae Sinica, 2013, 40 (4): 782-790.
[24] Gao Z H, Chen X Y, Lin C H, et al . Effect of fertilizer application rates on cassava N, P, K accumulations and allocation and yield in Sloping Lands of North Guangdong. Scientia Agricultura Sinica, 2011, 44(8): 1637-1645.
[25] Wang D Z, Zhang C J, Guo X S. Effects of lower fertilizer on rice growth and nitrogen use efficiency. Chinese Journal of Soil Science, 2012, 43(1): 161-165.
[26] Tang H, Zhang Y Z, Hou J Q, et al . Study on characteristics of growth and development and nutrients’ uptake of summer hot pepper under different fertilization amendments. Chinese Journal of Soil Science, 2012, 43(4): 891-895.
[27] Zhang J E, Gao A X, Xu H Q, et al . Effects of maize/peanut intercropping on rhizosphere soil microbes and nutrient contents. Chinese Journal of Applied Ecology, 2009, 20(7): 1597-1602.
[28] Zheng S L, Chen Q X, Mei F X, et al . Effect of bio-organic fertilizer on brassica alboglabra growth and soil N, P, K content. Journal of South China Agricultural University, 2007, 28(3):15-19.
[29] Zhu C H, Dong C X, Shen Q R, et al . Microbial mechanism on enhancement of inorganic fertilizer-N use efficiency for combined use of inorganic and organic fertilizers. Plant Nutrition and Fertilizer Science, 2010, 16(2): 282-288.
[30] Wang X J, Jia Z K, Liang L Y, et al . Effects of organic fertilizer application rate on leaf photosynthetic characteristics and grain yield of dry and maize. Chinese Journal of Applied Ecology, 2012, 23(2): 419-425.
[31] Winter K, Schramm M J. Analysis of stomatal and nonstomatal components in the environmental control of CO 2 exchanges in leaves of Welwitschia mirabilis . Plant Physiology, 1986, 82: 173-178.
[32] Farquhar G D, Sharkey T D.Stomatal conductance and photosynthesis. Annual Review of Plant Physiology, 1982, 33: 317-345.
[4] 段然, 汤月丰, 文炯, 等. 减量施肥对湖垸旱地作物产量及氮磷径流损失的影响. 中国生态农业学报, 2013, 21(5): 536-543.
[5] 孟琳, 张小莉, 蒋小芳, 等. 有机肥料氮替代部分化肥氮对稻谷产量的影响及替代率. 中国农业科学, 2009, 42(2): 532-542.
[6] 曹丹, 宗良纲, 肖峻, 等. 生物肥对有机黄瓜生长及土壤生物学特性的影响. 应用生态学报, 2010, 21(10): 2587-2592.
[8] 黄鹏, 何甜, 杜娟. 配施生物菌肥及化肥减量对玉米水肥及光能利用效率的影响. 中国农学通报, 2011, 27(3): 76-79.
[10] 赵庆雷, 吴修, 袁守江, 等. 长期不同施肥模式下稻田土壤磷吸附与解吸的动态研究. 草业学报, 2014, 23(1): 113-122.
[12] 罗世琼, 石安东, 袁玲, 等.黄花蒿抗疟相关成分及抗氧化活性对施肥方式的响应. 草业学报, 2014, 23(1): 339-345.
[13] 徐国伟, 李帅, 赵永芳, 等.秸秆还田与施氮对水稻根系分泌物及氮素利用的影响研究. 草业学报, 2014, 23(2): 140-146.
[14] 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2000.
[15] 贺立源, 梁华东. 高氯酸-硫酸消化植物样品防止氮素损失的研究. 分析化学, 1992, 20(11): 1277-1280.
[16] 邹琦.植物生理学实验指导[M]. 北京: 中国农业出版社, 2000.
[17] 易琼, 张秀芝, 何萍, 等. 氮肥减施对稻-麦轮作体系作物氮素吸收、利用和土壤氮素平衡的影响. 植物营养与肥料学报, 2010, 16(5): 1069-1077.
[18] 刘赵帆, 张国斌, 郁继华, 等. 氮肥形态及配比对花椰菜产量、品质和养分吸收的影响. 应用生态学报, 2013, 24(7): 1923-1930.
[19] 王冰清, 尹能文, 郑棉海, 等. 化肥减量配施有机肥对蔬菜产量和品质的影响. 中国农学通报, 2012, 28(1): 242-247.
[22] 王强, 姜丽娜, 符建荣, 等. 氮素形态、用量及施用时期对小青菜产量和硝酸盐含量的影响. 植物营养与肥料学报, 2008, 14(1): 126-131.
[23] 祝丽香, 毕建杰, 王建华, 等. 控释尿素与尿素配施对杭白菊产量和氮肥利用率的影响. 园艺学报, 2013, 40(4): 782-790.
[24] 高志红, 陈晓远, 林昌华, 等. 不同施肥水平对木薯氮磷钾养分积累、分配及其产量的影响. 中国农业科学, 2011, 44(8): 1637-1645.
[25] 王道中, 张成军, 郭熙盛. 减量施肥对水稻生长及氮素利用率的影响. 土壤通报, 2012, 43(1): 161-165.
[26] 汤宏, 张杨珠, 侯金权, 等. 不同施肥条件下夏季辣椒的生长发育与养分吸收规律研究. 土壤通报, 2012, 43(4): 891-895.
[27] 章家恩, 高爱霞, 徐华勤, 等. 玉米/花生间作对土壤微生物和土壤养分状况的影响. 应用生态学报, 2009, 20(7): 1597-1602.
[28] 郑少玲, 陈琼贤, 梅凤娴, 等. 生物有机肥对芥蓝生长及土壤中氮、磷、钾含量的影响. 华南农业大学学报, 2007, 28(3): 15-19.
[29] 朱菜红, 董彩霞, 沈其荣, 等. 配施有机肥提高化肥氮利用效率的微生物作用机制研究. 植物营养与肥料学报, 2010, 16(2): 282-288.
[30] 王晓娟, 贾志宽, 梁连友, 等. 不同有机肥量对旱地玉米光合特性和产量的影响. 应用生态学报, 2012, 23(2): 419-425.