不同营养调控对草地早熟禾生长和内源激素含量影响研究

doi:10.11686/cyxb20150204

摘要/Abstract

摘要： 养分在促进草坪生长中发挥着重要的作用。为了探讨不同肥料在草地早熟禾生长中的作用机理,采用不同的施肥组合进行草地早熟禾草坪的建植试验,研究了不同养分调控下草地早熟禾生长发育状况与内源激素含量水平的相关性,并对草地早熟禾坪用质量状况进行了评价。研究结果表明,在配合施用一定量生物菌肥和柠檬酸的情况下,较少量的氮、磷肥施用(处理3)能显著提高草地早熟禾坪用质量;各施肥组合能显著促进植株体IAA和ZT含量的提高,抑制ABA含量在根部的合成和体内的积累;在施肥后的35 d内,IAA和ZT含量随着施肥后时间的推移而不断增加,而ABA含量在除对照以外的各处理中相对较低,且随着施肥后时间的推移,其含量也明显下降,但在对照处理中却表现出一定的增加态势。

Abstract: Nutrient application is essential in turf management. To help reveal the mechanisms of fertilizer improvement in turfgrass growth, field experiments were carried out testing different fertilizer combinations. The relationship between endogenous hormone content and growth of kentucky bluegrass (Poa pratensis) was determined; turf quality was also evaluated. Fertilizer application (bio-fertilizer and citric acid) significantly affected turf quality. Fertilization significantly improved indoleacetic acid (IAA) and zeatin (ZT) content and restricted the synthesis and accumulation of abscisic acid (ABA).Post fertilization, IAA and ZT content increased with time (35 days). However, ABA content was relatively low and decreased after fertilizer application.

刘强, 张锁科, 孙万斌, 俞玲, 马晖玲. 不同营养调控对草地早熟禾生长和内源激素含量影响研究[J]. 草业学报, 2015, 24(2): 31-40.

LIU Qiang, ZHANG Suoke, SUN Wanbin, YU Ling, MA Huiling. Nutrition effects on growth and endogenous hormones in kentucky bluegrass[J]. Acta Prataculturae Sinica, 2015, 24(2): 31-40.

参考文献

[1] Gu M X, Zhang L, Shao L D, et al . Progress in somaclonal variation and its application in germplasm improvement of turfgrasses. Journal of Nuclear Agricultural Sciences, 2013, 27(4): 430-436.
[2] Duo L A, Zhao S L, Gao Y B. Probe into several ecological issues related to the establishment of urban turf systems. Acta Ecologica Sinica, 2007, 27(3): 1065-1071.
[3] Ma X M, Luo G F, Li D R, et al . Effects of various trampling stresses on turf characteristics and the natural recovery of Zoysia metrella . Acta Prataculturae Sinica, 2014, 23(3): 243-249.
[4] Evans L T, Bingham J, Jackson P, et al . Effects of awns and drought on the supply of photosynthate and its distribution within the wheat ears. Annals of Applied Biology, 1972, 70: 67-76.
[5] Wardlaw I F, Moncur L. Source, sink and hormonal control of translocation in wheat. Planta, 1976, 128: 93-100.
[6] Brenner M L, Cheikh N. The role of hormones in photosynthate partitioning and seed filling. In: Davies J. Plant Hormones[M]. Dordrecht: Kluwer Academic Publishers, 1995: 649-697.
[7] Marschner H. Mineral Nutrition of Higher Plants. 2nd edn[M]. London: Academic Press, 1995.
[8] Seth A K, Wareing P F. Hormone-directed transport of metabolites and its possible role in plant senescence. Journal of Experimental Botany, 1967, 18: 65-77.
[9] Bangerth F, Aufhammer W, Baum O. IAA level and dry matter accumulation at different positions within a wheat ear. Journal of Plant Physiology, 1985, 63: 121-125.
[10] Hartung W, Davies W J. Drought induced changes in physiology and ABA. In: Davies W J, Jones H G. Abacisic Acid, Physiology and Biochemistry[M]. Oxford: Bios Scientific Publishers, 1991: 63-79.
[11] Hartung W, Jeschke W D. Abacisic acid: a long distance stress signal in salt-stress plant. In: Lerner H R. Plants Response to Environmental Stress[M]. New York: Marcel Dekerker, 1999: 333-348.
[12] Xiong Y, Li Q K. Chinese Soil[M]. Beijing: Science Press, 1987: 492.
[13] Zhao Q L, Wu X, Yuan S J, et al . A study on the dynamics of phosphorus adsorption and desorption characteristics of paddy soil with long-term fertilization. Acta Prataculturae Sinica, 2014, 23(1): 113-122.
[14] Liu Q, Yao T, Ma H L. Combined effects of bio-fertilizer and citric acid on turf quality and soil biology on a calcareous soil. Acta Prataculturae Sinica, 2014, 23(5): 223-230.
[15] Zhang R L, Cai B Y. Effects of different fertilization tratments on the coverage density and turf grass growing time of hybrid Zoysia . Journal of Sichuan Grassland, 2004, (2): 32-34.
[16] Liu Y J, Han J G, Liu Y K. Effects of various fertilization treatments on turf quality of tall fescue. Grassland of China, 2001, 23(6): 27-33.
[17] Zhang M, Nyborg M, Malhi S S. Comparison of controlled-release nitrogen fertilizer on turfgrass in a moderate temperature area. Hort Science, 1998, 33(7): 1203-1206.
[18] Wang X Y, Hu T M, Wang Q Z, et al . Growth of kentucky bluegrass as influenced by nitrogen and trinexapac-ethyl. Scientia Agricultura Sinica, 2008, 41(11): 3679-3684.
[19] Han J G, Liu S, Liu Y J, et al . Effects of fertilization on turf quality of Poa pratensis and nitrate dynamic in soil. Acta Prataculturae Sinica, 2004, 13(6): 50-59.
[20] Fan W G, An H M, Liu G Q, et al . Changes of endogenous hormones contents in fruit, seeds and their effects on the fruit development of Rosa roxburghii . Agricultural Sciences in China, 2004, 37(5): 728-733.
[21] Liu J X. Approach to the comprehensive assessment system of turf quality-Ⅱ. utilization of the assessment system. Grassland of China, 2000, 65(3): 54-56.
[22] Zheng H J, Hua L, Gao Z G. A study on indices system and assessment of turf quality. Journal of Capital Normal University, 2003, 24(1): 78-82.
[23] Liu X J. A new method of evaluating turf quality-apparent quality evaluation. Journal of Gansu Agricultural University, 2004, 39(6): 651-655.
[24] Fan H R, Hua L, Wang H H. Study on indices system and assessment of turf quality. Pratacultural Science, 2006, 23(10): 101-105.
[25] Wang Y Z, Liu X W, Sun H Y, et al . Effects of water and nitrogen on root/shoot ratio and water use efficiency of winter wheat. Chinese Journal of Eco-Agriculture, 2013, 21(3): 282-289.
[26] Zhao H X, Huang X J, Zhu Z L, et al . Effect of mowing on the volume, weight and sugar content of root in mixed pasture of Bromus inermis and alfalfa. Grassland and Turf, 2007, (4): 50-53.
[27] Zhen J, Chen Q X, Jin C, et al . Discussion of different TTC methods for the determination of Liquidambar formosana and other broad-leaved tree container seedling root activity. Journal of Zhejiang Agricultural Sciences, 2008, (1): 39-42.
[28] Bai Y, Du F Y, Liu H W. Research progress on detection technique of plant hormone. Life Sciences, 2010, 22(1): 36-42.
[29] Ma Y N, Chen M X. Recent development in pretreatment and determination methods of plant endohormones. Chinese Agricultural Science Bulletin, 2011, 27(3): 15-19.
[30] Zhang Y M, Yan S J, Cao Z Z, et al . Study on the total saponin measurement and variation in growth period of four legumes. Pratacultural Science, 2009, 26(8): 122-127.
[31] Bian X J, Hu L, Li X L, et al . Effect of nitrogen and potassium levels on the growth and turf quality of perennial ryegrass. Acta Prataculturae Sinica, 2000, 9(1): 55-59.
[32] Wang X, Below F E. Cytokinins in enhanced growth and tillering of wheat induced by mixed nitrogen source. Crop Science, 1996, 36: 121-126.
[33] He Z L, Yuan K N, Zhu Z X. Effect of organic ligands on phosphat adsorption by hydrous iron and aluminum oxides, kaolinite and red earth. Acta Pedologica Sinica, 1990, 27(4): 377-384.
[34] Wang Y L, He Y Q, Li C L. Persistent activating effect of citric acid on phosphorus in red soil and its mechanism. Acta Pedologica Sinica, 2007, 44(1): 130-135.
[35] Wei M L, Huang T W, Sun Y Q, et al . Effects of shiqi bio-bacterial fertilizer on Panax notoginseng growth. Special Wild Economic Animal and Plant Research, 2008, 30(3): 61-64.
[36] Li Z G, Wang X M, Liu T Y, et al . Restoration of continuous cropping obstacles of rehmannia glutinosa libosch by applying compound bacterial manure. Hunan Agricultural Sciences, 2008, (5): 62-65.
[37] Huang J, Wang G F, An S Z, et al . Effect of nitrogen fertilization on the vegetation structure and biomass of degraded meadow and soil fertility. Pratacultural Science, 2009, 26(3): 75-75.
[38] Ji Y, Zhang Q X, Lin H M, et al . Effect of nitrogen on growth and total alkaloids of Sophora flavescens . Acta Prataculturae Sinica, 2009, 18(3): 159-164.
[39] Lin H M, Ji Y, Wang B, et al . Effects of nitrogen and phosphorus on seedling growth and total alkaloids of Sophora flavescens . Acta Prataculturae Sinica, 2010, 19(3): 102-109.
[40] Li X W, Men M X, Wang S T, et al . The effects of long-term fertilization on crop yields and farmland nutrient equilibrium. Acta Prataculturae Sinica, 2009, 18(1): 9-16.
[41] Yean D Y. Effect of fertilizer application on seed production in zoysia grass. Journal of the Korean Society for Horticultural Science, 1987, 28(2): 55-57.
[42] He P, Jin J Y. Effect of N and K nutrition on changes of endogenous hormone and metabolism of active oxygen during leaf senescence in spring maize. Plant Nutrition and Fertilizer Science, 1999, 5(4): 289-296.
[43] Guo Y, Song X L, Wang Q C, et al . Effect of potassium nutrition on endogenous hormone and free radicals in cotton seedling. Acta Agriculturae Boreali-Sinica, 2006, 21(1): 59-62.
[44] Li L Y, Cui G X. Progress in the research on the changes of plant endogenous hormone under nutrient stress. Crop Research, 2002, (5): 240-244.
[45] Johson J F, Vance C P, Allan D L. Phosphate deficiency in Lupinus albus . Plant Physiology, 1996, 112(1): 31-41.
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[2] 多立安, 赵树兰, 高玉葆. 草坪建植体系构建中的生态问题. 生态学报, 2007, 27(3): 1065-1071.
[3] 马雪梅, 罗赣丰, 李德荣, 等. 践踏胁迫对沟叶结缕草坪用性状的影响及其自然恢复能力. 草业学报, 2014, 23(3): 243-249.
[12] 熊毅, 李庆逵. 中国土壤[M]. 北京: 科学出版社, 1987: 492.
[13] 赵庆雷, 吴修, 袁守江, 等. 长期不同施肥模式下稻田土壤磷吸附与解吸的动态研究. 草业学报, 2014, 23(1): 113-122.
[14] 刘强, 姚拓, 马晖玲. 菌肥与柠檬酸互作对石灰性土壤生物学特性及草坪质量的影响. 草业学报, 2014, 23(5): 223-230.
[15] 张如莲, 蔡碧云. 不同施肥处理对杂交结缕草草坪盖度及成坪时间的影响. 四川草原, 2004, (2): 2-34.
[16] 刘玉杰, 韩建国, 刘雨坤. 不同施肥处理对高羊茅草坪质量的影响. 中国草地, 2001, 23(6): 27-33.
[18] 王晓英, 呼天明, 王佺珍, 等. 氮肥和Trinexapac-ethyl配合施用对草地早熟禾草坪生长的影响. 中国农业科学, 2008, 41(11): 3679-3684.
[19] 韩建国, 刘帅, 刘玉杰, 等. 施肥对草地早熟禾草坪质量及土壤中硝态氮动态的影响. 草业学报, 2004, 13(6): 50-59.
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[21] 刘建秀. 草坪坪用价值综合评价体系的探讨-Ⅱ. 评价体系的应用. 中国草地, 2000, 65(3): 54-56.
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[23] 刘晓静. 草坪质量评价新方法——综合外观质量法. 甘肃农业大学学报, 2004, 39(6): 651-655.
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[25] 王艳哲, 刘秀位, 孙宏勇, 等. 水氮调控对冬小麦根冠比和水分利用效率的影响研究. 中国生态农业学报, 2013, 21(3): 282-289.
[26] 赵海新, 黄晓群, 朱占林, 等. 刈割对无芒雀麦、苜蓿混播草层根体积根重及含糖量的影响. 草原与草坪, 2007, (4): 50-53.
[27] 郑坚, 陈秋夏, 金川, 等. 不同TTC法测定枫香等阔叶树容器苗根系活力探讨. 浙江农业科学, 2008, (1): 39-42.
[28] 白玉, 杜甫佑, 刘虎威. 植物激素检测技术研究进展. 生命科学, 2010, 22(1): 36-42.
[29] 马有宁, 陈铭学. 植物内源激素预处理方法与色谱检测技术的研究进展. 中国农学通报, 2011, 27(3): 15-19.
[30] 张咏梅, 晏石娟, 曹致中, 等. 4种豆科牧草总皂苷含量的测定及其生长过程中含量的变化. 草业科学, 2009, 26(8): 122-127.
[31] 边秀举, 胡林, 李晓林, 等. 不同氮钾用量对多年生黑麦草草坪的影响. 草业学报, 2000, 9(1): 55-59.
[33] 何振立, 袁可能, 朱祖祥. 有机阴离子对磷酸根吸附的影响. 土壤学报, 1990, 27(4): 377-384.
[34] 王艳玲, 何园球, 李成亮. 柠檬酸对红壤磷的持续活化效应及其活化机理的探讨. 土壤学报, 2007, 44(1): 130-135.
[35] 韦美丽, 黄天卫, 孙玉琴, 等. 世奇微生物菌肥对三七生长及产量质量的影响. 特产研究, 2008, 30(3): 61-64.
[36] 李自刚, 王新民, 刘太宇, 等. 复合微生物菌肥对怀地黄连作障碍修复机制研究. 湖南农业科学, 2008, (5): 62-65.
[37] 黄军, 王高峰, 安沙舟, 等. 施氮对退化草甸植被结构和生物量及土壤肥力的影响. 草业科学, 2009, 26(3): 75-75.
[38] 纪瑛, 张庆霞, 蔺海明, 等. 氮肥对苦参生长和生物总碱的效应. 草业学报, 2009, 18(3): 159-164.
[39] 蔺海明, 纪瑛, 王斌, 等. 生荒地氮磷配施对苦参苗生长和苦参总碱含量的影响. 草业学报, 2010, 19(3): 102-109.
[40] 李新旺, 门明新, 王树涛, 等. 长期施肥对华北平原潮土作物产量及农田养分平衡的影响. 草业学报, 2009, 18(1): 9-16.
[42] 何萍, 金继运. 氮钾营养对春玉米叶片衰老过程中激素变化与活性氧代谢的影响. 植物营养与肥料学报, 1999, 5(4): 289-296.
[43] 郭英, 宋宪亮, 王庆材, 等. 施钾对棉花苗期叶片内源激素与氧自由基代谢的影响. 华北农学报, 2006, 21(1): 59-62.
[44] 李良勇, 崔国贤. 营养胁迫下植物内源激素变化研究进展. 作物研究, 2002, (5): 240-244.