外源甜菜碱对匍匐翦股颖的抗旱性调控作用分析

doi:10.11686/cyxb20150308

摘要/Abstract

摘要： 为了探究外源甜菜碱(GB)对干旱胁迫及复水条件下匍匐翦股颖的生长调控作用，以匍匐翦股颖(T-1)成熟草皮为材料，移栽至温室适应性生长50 d后，分别用0，50，100，200 mmol/L的GB喷施匍匐翦股颖叶片，3 d后进行人工模拟干旱处理和复水处理，研究在不同水分条件下，外源GB对匍匐翦股颖坪观质量、叶片相对含水量、细胞膜透性、叶绿素、游离脯氨酸、可溶性蛋白、丙二醛(MDA)含量以及抗氧化酶活性的影响。结果表明，干旱胁迫降低了匍匐翦股颖的坪观质量、相对含水量和叶绿素含量，提高了细胞膜透性、游离脯氨酸含量、可溶性蛋白含量、MDA含量和SOD、CAT、POD活性，而外施一定浓度的GB可使干旱胁迫引起的坪观质量、叶片相对含水量和叶绿素含量的下降幅度显著降低，同时相对提高了叶片脯氨酸含量、可溶性蛋白含量以及SOD和CAT活性，并降低了细胞膜透性和MDA含量，复水后，各指标均得到不同程度的恢复，其中GB处理恢复甚至优于干旱处理前的水平。本研究结果表明，外施一定浓度的GB对干旱胁迫下匍匐翦股颖的抗旱性具有增效作用，并且在复水后使匍匐翦股颖积累了更多对干旱逆境的防御能力，使其能够更好地适应干旱胁迫环境。综合各项指标，100 mmol/L的GB处理最有利于提高匍匐翦股颖的抗旱性。

Abstract: To explore the regulatory role of exogenous glycine betaine (GB) on the growth of creeping bentgrass (Agrostis stolonifera) under drought stress and rehydration, creeping bentgrass sods were transplanted into a greenhouse and grown for 50 days before spraying with GB using 4 different concentrations (0, 50, 100 and 200 mmol/L). Artificial drought treatment and rehydration were applied 3 days after GB application. Turf quality, relative water content, cell membrane permeability, chlorophyll content, free-proline content, soluble protein content, malondialdehyde (MDA) content, and the activity of antioxidant enzymes; superoxide dismutase (SOD); catalase (CAT); and peroxidase (POD) were measured. The results showed that turf quality, relative water content and chlorophyll content were decreased by drought stress while cell membrane permeability, free-proline content, soluble protein content, MDA content and SOD, CAT, and POD activity were increased. However, application of GB significantly reduced the decline of turf quality, relative water content, and chlorophyll content under drought stress. Application of GB also increased the free-proline content, soluble protein content, SOD and CAT activity but reduced cell membrane permeability and MDA content compared to the control. Recovery after rehydration was also improved by GB application. The results suggested that exogenous GB could enhance the drought tolerance of creeping bentgrass under drought stress and the optimum concentration was100 mmol/L.

刘思露，杨鹏，尹淑霞. 外源甜菜碱对匍匐翦股颖的抗旱性调控作用分析[J]. 草业学报, 2015, 24(3): 80-88.

LIU Silu, YANG Peng, YIN Shuxia. Regulatory role of exogenous glycine betaine on the drought tolerance of creeping bentgrass[J]. Acta Prataculturae Sinica, 2015, 24(3): 80-88.

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