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草业学报 ›› 2016, Vol. 25 ›› Issue (2): 160-168.DOI: 10.11686/cyxb2015143

• 研究论文 • 上一篇    下一篇

内生真菌和水引发对NaCl胁迫条件下中华羊茅种子萌发的影响

旷宇, 南志标, 田沛*   

  1. 草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃 兰州 730020
  • 收稿日期:2015-03-17 出版日期:2016-02-20 发布日期:2016-02-20
  • 通讯作者: E-mail:tianp@lzu.edu.cn
  • 作者简介:旷宇(1989-),女,湖南攸县人,在读硕士。E-mail:kuangy13@lzu.edu.cn
  • 基金资助:
    国家重点基础研究发展规划(973计划) (2014CB138702)和中央高校基本科研业务费专项(lzujbky-2014-76)资助

Effects of Epichloё endophyte and seed hydro-priming on the germination of Festuca sinensis under NaCl stress

KUANG Yu, NAN Zhi-Biao, TIAN Pei*   

  1. State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2015-03-17 Online:2016-02-20 Published:2016-02-20

摘要: 本试验以带Epichloё内生真菌(E+)及不带内生真菌(E-)中华羊茅种子为研究材料,研究其水引发后,在NaCl胁迫条件下的萌发情况。结果表明,NaCl胁迫显著降低中华羊茅种子发芽率、发芽势、发芽指数、胚芽长、胚根长和干重。除对照处理外,相对于不带内生真菌的中华羊茅种子,内生真菌能显著提高不同NaCl浓度胁迫条件下中华羊茅种子的萌发力及幼苗生长。水引发与内生真菌可产生互作,在引发时间为20 min时与内生真菌的互作效果最为明显,在不同NaCl浓度胁迫下中华羊茅种子各萌发指标及幼苗生长情况均显著高于其他处理。在对各指标的相关性分析显示,中华羊茅种子的发芽率、发芽势、发芽指数、胚芽长、胚根长和干重相互之间均呈极显著正相关。

Abstract: This study investigated the germination and vigor of Festuca sinensis seeds infected (E+) and uninfected (E-) with Epichloё endophyte and under different levels of NaCl stress after hydro-priming. The results showed that NaCl stress treatments significantly reduced seed germination rate, interim germination, the germination index, the length of embryo and radicle and the dry weights of both E+ and E- F. sinensis seeds. Compared with E-, infection with Epichloё endophyte can significantly improve germination and seedling growth of F. sinensis seeds under different concentrations of NaCl. There were interaction effects between seed hydro-priming and the Epichloё endophyte. The highest levels of interaction were with 20 mins seed hydro-priming, when the germination indices and seedling growth rates were significantly higher than for all the other treatments. Correlation analysis showed that F. sinensis seed germination rate, interim germination, germination index, the length of embryo and radicle and dry weight were all significantly positively related to each other.