外源NO对NaCl胁迫下夏枯草幼苗抗氧化能力及光合特性的影

doi:10.11686/cyxb2015454

草业学报 ›› 2016, Vol. 25 ›› Issue (7): 121-130.DOI: 10.11686/cyxb2015454

外源NO对NaCl胁迫下夏枯草幼苗抗氧化能力及光合特性的影

常青山¹, 张利霞^{2, *}, 杨伟³, 周姗姗¹, 黄青哲¹, 吕凤娟¹, 黄玥¹, 葛淑慧¹, 张天蒙¹

1.河南科技大学林学院,河南洛阳 471003；
2.河南科技大学农学院,河南洛阳 471003；
3.扬子江药业集团江苏龙凤堂中药有限公司,江苏泰州 225321

收稿日期:2015-09-23 修回日期:2015-11-23 出版日期:2016-07-20 发布日期:2016-07-20
通讯作者: hkdzlx@126.com
作者简介:常青山(1979-),男,河南安阳人,讲师,博士。E-mail: hkdcqs@126.com
基金资助:
河南省高等学校重点科研资助项目(15A180037,16A220005),河南省科技攻关计划项目(162102110095),河南科技大学高级别项目培育基金(2015GJB029),河南科技大学青年基金资助项目(4024-13350066,4026-13350041),河南科技大学博士科研启动基金资助项目(4024-13480054,4026-13480038)和河南科技大学大学生研究训练计划(SRTP)项目(2014254,2014281,2015152)资助

Effects of exogenous nitric oxide on antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under NaCl stres

CHANG Qing-Shan¹, ZHANG Li-Xia^{2, *}, YANG Wei³, ZHOU Shan-Shan¹, HUANG Qing-Zhe¹, LV Feng-Juan¹, HUANG Yue¹, GE Shu-Hui¹, ZHANG Tian-Meng¹

1.College of Forestry, Henan University of Science and Technology, Luoyang 471003, China;
2.College of Agriculture, Henan University of Science and Technology, Luoyang 471003, China;
3.Jiangsu Longfeng Hall Chinese Medicine Limited Company, Yangtze River Pharmaceutical Group, Taizhou 225321, China

Received:2015-09-23 Revised:2015-11-23 Online:2016-07-20 Published:2016-07-20

摘要/Abstract

摘要： 试验采用硝普钠(SNP)为NO供体,夏枯草幼苗为材料,研究外源0.01~0.50 mmol/L SNP对70 mmol/L NaCl胁迫下夏枯草幼苗抗氧化系统、光合参数与叶绿素荧光参数的影响。结果表明,0.05~0.10 mmol/L SNP可以缓解NaCl胁迫对夏枯草幼苗造成的伤害,其中0.10 mmol/L SNP 缓解效果最显著,该处理显著提高了NaCl胁迫下夏枯草幼苗叶片的过氧化物酶(POD)、过氧化氢酶(CAT)活性,降低了电导率与丙二醛(MDA)的含量。显著提高了夏枯草叶片叶绿素a含量、叶绿素b含量、总叶绿素含量、净光合速率(P_n)、气孔导度(G_s)与蒸腾速率(T_r),降低了胞间CO₂浓度(C_i)。叶绿素荧光动力学参数显示,0.10 mmol/L SNP处理显著降低了NaCl胁迫下夏枯草幼苗的初始荧光(F_o)与非光化学荧光猝灭系数(NPQ),提高了最大荧光(F_m)、PSⅡ潜在光化学效率(F_v/F_o)、PSⅡ最大光化学效率(F_v/F_m)和PSⅡ有效光化学量子产量(F_v'/F_m')。外源NO通过提高抗氧化酶活性,来减少脂质过氧化作用,减少光抑制对PSⅡ的破坏,提高光化学能力,增强夏枯草叶片的光合能力,从而最终提高夏枯草的抗盐能力。本试验条件下,以0.10 mmol/L SNP处理效果最为显著。

Abstract: The objective of these experiments was to determine the effects of exogenous nitric oxide (NO) on the antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under salt stress. Seedlings of P. vulgaris under salt stress (70 mmol/L NaCl) were treated with sodium nitroprusside (SNP, an exogenous NO donor) at different concentrations (0.01, 0.05, 0.10 and 0.50 mmol/L) and their antioxidant enzyme activity, photosynthetic pigment contents, gas exchange, and chlorophyll fluorescence characteristics were analyzed. The results showed that 0.05-0.10 mmol/L SNP alleviated symptoms of NaCl stress in P. vulgaris seedlings. The 0.10 mmol/L SNP treatment produced the best results. The seedlings in this treatment showed significantly increased (P<0.05) activities of peroxidase and catalase, decreased malondialdehyde content and electric conductivity, significantly increased (P<0.05) chlorophyll content, net photosynthetic rate, stomatal conductance, and transpiration rate, and decreased intercellular CO₂ concentration. Analyses of chlorophyll fluorescence data showed that the 0.10 mmol/L SNP treatment resulted in a significant decrease (P<0.05) in initial fluorescence and non-photochemical quenching, and increased maximum fluorescence, photosystem Ⅱ (PSⅡ) potential fluorescence efficiency, PSⅡ maximum fluorescence efficiency, and PSⅡ excitation capture efficiency in leaves of salt-stressed P. vulgaris seedlings. These results showed that application of SNP at certain concentrations stabilized membranes by increasing the activities of antioxidant enzymes, and improved photochemical efficiency. These changes alleviated photoinhibition damage resulting from salt damage to the PSⅡ center, and enhanced the photosynthetic capacity to increase the salt resistance of P. vulgaris seedlings. The 0.10 mmol/L SNP treatment had the greatest effect on improving salt tolerance of P. vulgaris seedlings under these experimental conditions.

常青山, 张利霞, 杨伟, 周姗姗, 黄青哲, 吕凤娟, 黄玥, 葛淑慧, 张天蒙. 外源NO对NaCl胁迫下夏枯草幼苗抗氧化能力及光合特性的影[J]. 草业学报, 2016, 25(7): 121-130.

CHANG Qing-Shan, ZHANG Li-Xia, YANG Wei, ZHOU Shan-Shan, HUANG Qing-Zhe, LV Feng-Juan, HUANG Yue, GE Shu-Hui, ZHANG Tian-Meng. Effects of exogenous nitric oxide on antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under NaCl stres[J]. Acta Prataculturae Sinica, 2016, 25(7): 121-130.

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外源NO对NaCl胁迫下夏枯草幼苗抗氧化能力及光合特性的影

Effects of exogenous nitric oxide on antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under NaCl stres

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