小麦秸秆与米糠粕瘤胃体外发酵组合效应研究

doi:10.11686/cyxb2015516

摘要/Abstract

摘要： 为了探讨小麦秸秆与米糠粕间的组合效应,采用体外产气法评价了小麦秸秆与米糠粕按100:0(RBM0)、75:25(RBM25)、50:50(RBM50)、25:75(RBM75)、0:100(RBM100)的比例组合后的发酵特性。结果表明,1)在产气特性方面,随着米糠粕比例的增加,12,24,48 h累计产气量(GP)、理论最大产气量(HM)和产气速率(B)逐渐增大,而体外延滞时间(Lag)逐渐降低,各比例之间差异显著(P<0.05)。2)在体外发酵12,24,48 h时,当米糠粕占25%时,小麦秸秆与米糠粕的产气量产生最大组合效应(P<0.05)。3)当米糠粕占75%时,小麦秸秆与米糠粕的干物质降解率(DMD)、中性洗涤纤维降解率(NDFD)、酸性洗涤纤维降解率(ADFD)最大,各比例之间差异显著(P<0.05);当米糠粕占25%时,小麦秸秆与米糠粕的DMD产生最大的组合效应(P<0.05);当米糠粕占75%时,小麦秸秆与米糠粕的NDFD、ADFD产生最大组合效应(P<0.05)。4)当米糠粕占75%时,小麦秸秆与米糠粕组合体外发酵48 h的乙酸、丙酸、丁酸以及总挥发性脂肪酸浓度最高,乙酸/丙酸最低。5)48 h累计产气量、产气速率与干物质(DM)、粗蛋白(CP)、粗灰分(Ash)以及中性洗涤可溶物(NDS)极显著正相关(P<0.01),与中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、有机物(OM)、半纤维(HC)以及中性洗涤可溶物/粗蛋白(NDS/CP)呈极显著负相关(P<0.01);理论最大产气量与DM、CP、Ash、NDS显著正相关(P<0.05),与NDF、ADF、OM和HC显著负相关(P<0.05);Lag与NDS/CP极显著正相关(P<0.01)。本试验结果提示,在本试验条件下,从产气量及干物质降解率的组合效应方面来看,小麦秸秆与米糠粕的最优组合是75:25;从纤维降解率的组合效应方面来看,小麦秸秆与米糠粕的最优组合是25:75。

Abstract: This study used an in vitro gas production technique to investigate the associative effects of wheat straw (WS) and rice bran meal (RBM), on the fermentation characteristics of WS mixed with RBM at rations of 100:0 (RBM0), 75:25 (RBM25), 50:50 (RBM50), 25:75 (RBM75) and 0:100 (RBM100). Key results were:1) For gas measurements, 12, 24 and 48 h gas production (GP), maximum theoretical (HM) and rate of biogas production (B) increased while the lag time reduced incrementally (P<0.05) with the percentage of RBM added; 2) The RBM25 group had the highest positive associative effects for B (P<0.05); 3) For dry matter degradation (DMD), neutral detergent fiber degradation (NDFD) and acid detergent fiber degradation (ADFD),the RBM75 group had the highest values (P<0.05), while for DMD, the RBM25 group had the highest positive associative effects (P<0.05). For NDFD and ADFD, the RBM75 group had the highest positive associative effects (P<0.05); 4) With the RBM75 mixture, the acetate, propionate, butyrate and total volatile fatty acid production were highest, and acetate:propionate was the lowest; 5) For the 48 h GP, B was positively related to dry matter (DM), crude protein (CP), crude ash (Ash) and neutral detergent soluble (NDS) (P<0.01), but was negatively related to neutral detergent fiber (NDF), acid detergent fiber (ADF), organic matter (OM), hemicellulose (HC) and NDS/CP (P<0.01); HM was positively related to DM, CP, Ash, NDS (P<0.05), but it was negatively related to NDF, ADF, OM and HC (P<0.05); the lag was negatively related to NDS/CP (P<0.01). It is concluded that for B and DMD, the RBM25 mixture gave the best associative effects in this experiment. For fiber degradation rate, a RBM75 mixture gave the best associative effects.

孟梅娟, 涂远璐, 白云峰, 严少华, 高立鹏, 刘萍. 小麦秸秆与米糠粕瘤胃体外发酵组合效应研究[J]. 草业学报, 2016, 25(9): 161-172.

MENG Mei-Juan, TU Yuan-Lu, BAI Yun-Feng, YAN Shao-Hua, GAO Li-Peng, LIU Ping. Study of associative effects of wheat straw mixed with rice bran meal on in vitro fermentation parameters[J]. Acta Prataculturae Sinica, 2016, 25(9): 161-172.

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