苜蓿茎点霉对紫花苜蓿光合生理的影响

doi:10.11686/cyxb2016275

草业学报 ›› 2017, Vol. 26 ›› Issue (1): 112-121.DOI: 10.11686/cyxb2016275

苜蓿茎点霉对紫花苜蓿光合生理的影响

樊秦^1,2, 李彦忠^1,3,*

1.草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020;
2.甘肃中医药大学,甘肃兰州 730000;
3.中国农业科学院草原研究所,内蒙古呼和浩特 010010

收稿日期:2016-07-14 出版日期:2017-01-20 发布日期:2017-01-20
通讯作者: E-mail:liyzh@lzu.edu.cn
作者简介:樊秦(1976-), 女, 重庆酉阳人,副教授,在读博士。E-mail:fanqin408@126.com
基金资助:
公益性行业(农业)科研专项(201303057),国家牧草产业技术体系(CARS-35)和国家自然科学基金(31272496)资助

The effect of Phoma medicaginis on the photosynthetic physiology of Medicago sativa

FAN Qin^1,2, LI Yan-Zhong^1,3,*

1.State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.Gansu University of Chinese Medicine, Lanzhou 730000, China;
3.Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China

Received:2016-07-14 Online:2017-01-20 Published:2017-01-20

摘要/Abstract

摘要：

苜蓿茎点霉侵染紫花苜蓿后严重影响其产量和质量。本研究采用植物生长室盆栽法,利用光合作用测量系统研究苜蓿茎点霉对紫花苜蓿叶片光合生理的影响。结果表明, 除侵染第27天外,苜蓿茎点霉使紫花苜蓿叶片净光合速率(P_n)、气孔导度(G_s)和蒸腾速率(T_r)降低,对胞间二氧化碳浓度(C_i)在侵染前期影响不大。在侵染至23 d,紫花苜蓿叶片光化学量子效率(F_v/F_m)、PSⅡ反应中心激发能捕获效率(F_v'/F_m')、PSⅡ反应中心电荷分离实际量子效率(Φ_PSⅡ)、电子传递速率(ETR)、光化学猝灭系数(qP)和非光化学淬灭系数(qN)显著降低。光补偿点 (LCP)、CO₂补偿点(CCP)和羧化效率(CE)降低。表明苜蓿茎点霉通过降低紫花苜蓿叶片中光合电子传递率捕获效率、非光化学猝灭系数(qN)和羧化效率(CE),而产生光抑制,使CO₂的同化作用降低,影响紫花苜蓿的生长发育。

Abstract:

The yield and quality of Medicago sativa are seriously affected by the fungal pathogen Phoma medicaginis. A study has been undertaken using growth chamber pot experiments and the LI-6400/XT photosynthesis measurement system to investigate the effects of this pathogen on the photosynthetic physiology of M. sativa. The results showed that until 27 days post-inoculation (dpi) the net photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) of M. sativa were reduced by P. medicaginis infection. In the early stages of infection there were little changes in intercellular CO₂ concentration (C_i) in the leaves. By 23 dpi, infected leaves showed significant reductions in photochemical quantum efficiency (F_v/F_m), PSⅡ reaction center of excitation energy capture efficiency (F_v'/F_m'), the relative quantum efficiency of PSⅡ photochemistry (Φ_PSⅡ), electron transfer rate (ETR), photochemical quenching (qP) and non-photochemical quenching (qN). Light compensation point, CO₂ compensation point and carboxylation efficiency (CE) also decreased. The results indicate that the photosynthetic electron transfer rate and capture efficiency, non-photochemical quenching (qN), and carboxylation efficiency (CE) decreased in leaves infected by P. medicaginis, leading to light suppression and a decrease in the assimilation of CO₂ that affect the growth and development of M. sativa.

樊秦, 李彦忠. 苜蓿茎点霉对紫花苜蓿光合生理的影响[J]. 草业学报, 2017, 26(1): 112-121.

FAN Qin, LI Yan-Zhong. The effect of Phoma medicaginis on the photosynthetic physiology of Medicago sativa[J]. Acta Prataculturae Sinica, 2017, 26(1): 112-121.

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苜蓿茎点霉对紫花苜蓿光合生理的影响

The effect of Phoma medicaginis on the photosynthetic physiology of Medicago sativa

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