禾草内生真菌与2种AM真菌互作对黑麦草生长的影响

doi:10.11686/cyxb2016445

草业学报 ›› 2017, Vol. 26 ›› Issue (9): 132-140.DOI: 10.11686/cyxb2016445

禾草内生真菌与2种AM真菌互作对黑麦草生长的影响

李芳, 李彦忠, 段廷玉^*

草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2016-11-29 修回日期:2017-03-22 出版日期:2017-09-20 发布日期:2017-09-20
通讯作者: *通信作者Corresponding author. E-mail:duanty@lzu.edu.cn
作者简介:李芳(1990-),女,青海西宁人,在读博士。E-mail:lif2013@lzu.edu.cn
基金资助:
中央高校基本科研业务费(2022016zr0003),国家绿肥产业技术体系(CARS-22)和国家自然科学基金项目(31100368/C0309)资助

Effects of interactions between a grass endophyte and an arbuscular mycorrhizal fungus on perennial ryegrass growth

LI Fang, LI Yan-Zhong, DUAN Ting-Yu^*

State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2016-11-29 Revised:2017-03-22 Online:2017-09-20 Published:2017-09-20

摘要/Abstract

摘要： 在温室盆栽条件下,以侵染内生真菌和未侵染内生真菌的黑麦草为材料,接种幼套球囊霉(Claroideoglomus etunicatum)和根内球囊霉(Rhizophagus intraradices)以及两种AM真菌的混合菌剂,通过比较单一及混合AM真菌和禾草内生真菌对黑麦草株高、根长、生物量、P含量、可溶性糖、可溶性蛋白以及光合指标等方面的差异,探究AM真菌与禾草内生真菌对黑麦草生长的影响。结果表明,接种幼套球囊霉、根内球囊霉和混合菌剂的黑麦草平均AM真菌侵染率分别为44.04%,34.13%和41.13%,禾草内生真菌降低AM真菌平均侵染率22.28%。接种AM真菌和侵染禾草内生真菌均提高了黑麦草形态和生理生化指标,且二者(尤其是幼套球囊霉与禾草内生真菌)共存时,黑麦草的形态和生理生化指标最高,与既不侵染禾草内生真菌也不接AM真菌的处理相比,幼套球囊霉与禾草内生真菌共同作用时总P含量、总生物量和净光和速率分别提高48.21%, 21.12%和73.53%,总可溶性糖和蛋白分别提高147.12%和24.27%。总体而言,AM真菌显著促进了植物地上、地下部分生长,而禾草内生真菌主要促进植物地上部分的生长。

Abstract: The aim of this study was to determine the effects of interactions between an arbuscular mycorrhizal (AM) fungus and an endophyte on the growth of perennial ryegrass (Lolium perenne). Ryegrass plants were grown in pots in a greenhouse and inoculated with the AM fungus Claroideoglomus etunicatum, Rhizophagus intraradices, and a mixture of C. etunicatum and R. intraradices, and then plant height, root length, biomass, phosphorus content, soluble sugars content, soluble protein content, and photosynthetic indexes were monitored. The results showed that the average AM colonization by C. etunicatum, R. intraradices, and the mixture of both species was 44.04%, 34.13%, and 41.13%, respectively. The average AM colonization was 22.28% lower in endophyte-infected ryegrass than in endophyte-free ryegrass. Both the AM fungus and the endophyte increased the morphological, physiological, and biochemical indexes of perennial ryegrass. The total P content, total dry weight, photosynthetic rate, total soluble sugars content, and soluble protein content was 48.21%, 21.12%, 73.53%, 147.12%, and 24.27% higher, respectively, in the plants co-inoculated with C. etunicatum and grass endophyte than in uninoculated plants. Both fungi enhanced shoot growth and root growth, but in general, the AM fungus significantly increased shoot and root growth, while the endophyte mainly increased the shoot growth of perennial ryegrass.

李芳, 李彦忠, 段廷玉. 禾草内生真菌与2种AM真菌互作对黑麦草生长的影响[J]. 草业学报, 2017, 26(9): 132-140.

LI Fang, LI Yan-Zhong, DUAN Ting-Yu. Effects of interactions between a grass endophyte and an arbuscular mycorrhizal fungus on perennial ryegrass growth[J]. Acta Prataculturae Sinica, 2017, 26(9): 132-140.

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禾草内生真菌与2种AM真菌互作对黑麦草生长的影响

Effects of interactions between a grass endophyte and an arbuscular mycorrhizal fungus on perennial ryegrass growth

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