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草业学报 ›› 2017, Vol. 26 ›› Issue (12): 170-178.DOI: 10.11686/cyxb2017065

• 研究论文 • 上一篇    下一篇

2,3-丁二醇诱导下匍匐翦股颖叶片细胞结构变化及抗病相关性分析

刘兴菊1, 2, 3, 马源1, 2, 3, 马晖玲1, 2, 3, *, 张咏梅4, 余倩倩5   

  1. 1.甘肃农业大学草业学院,甘肃 兰州 730070;
    2.草业生态系统教育部重点实验室,甘肃 兰州 730070;
    3.中-美草地畜牧业可持续发展研究中心,甘肃 兰州 730070;
    4.甘肃农业大学研究测试中心, 甘肃 兰州 730070;
    5.华南农业大学园艺学院,广东 广州 510642
  • 收稿日期:2017-02-24 修回日期:2017-03-29 出版日期:2017-12-20 发布日期:2017-12-20
  • 通讯作者: E-mail:mahl@gsau.edu.cn
  • 作者简介:刘兴菊(1990-),女,甘肃白银人,在读硕士。 E-mail:1437415960@qq.com
  • 基金资助:
    国家自然科学基金项目(31360583)资助

The correlation between cell microstructure induced by 2,3-butanediol and fungal resistance of creeping bentgrass

LIU Xing-Ju1, 2, 3, MA Yuan1, 2, 3, MA Hui-Ling1, 2, 3, *, ZHANG Yong-Mei4, YU Qian-Qian5   

  1. 1.Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China;
    2.Key Laboratory of Grassland Ecosystem, Lanzhou 730070, China;
    3.Sino U.S.Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
    4. Instrumental Research & Analysis Center, Gansu Agricultural University, Lanzhou 730070, China;
    5. College of Horticulture, South China Agricultural University, Guangzhou 510642, China
  • Received:2017-02-24 Revised:2017-03-29 Online:2017-12-20 Published:2017-12-20
  • Contact: E-mail:mahl@gsau.edu.cn

摘要: 由立枯丝核菌(Rhizoctonia solani)引起的褐斑病是匍匐翦股颖草坪最常见的病害之一,2,3-BD(2,3-butanediol)可诱导植物产生抗病反应,提高植物的抗病性。本试验采用250 μmol/L 2,3-BD诱导匍匐翦股颖对褐斑病的抗性,以石蜡切片方法通过显微观察,测定接种立枯丝核菌后匍匐翦股颖叶片细胞显微结构的变化。结果表明,接种病原菌后匍匐翦股颖病情指数第15天达到70.45%,2,3-BD诱导后病情指数显著降低至21.63%。与未诱导接菌处理相比,诱导后匍匐翦股颖叶片细胞结构排列紧密,气腔变小,有较多乳突形成,上、下表皮细胞显著增大,第7天差异最显著,分别是其1.19和1.26倍;叶肉细胞大小于第15天差异最为显著,是其1.30倍;叶绿体数量明显增加,第1、7、15天分别是未诱导接菌处理的1.25、1.20、1.24倍;维管束和导管大小在各时间段均变化不明显,但木质化程度较高,皮层组织加厚。综上所述,250 μmol/L 的2,3-BD能诱导匍匐翦股颖对褐斑病的抗性,并使细胞结构发生变化,其中叶片上、下表皮细胞、叶肉细胞大小和叶绿体数目与抗病性呈正相关性。

Abstract: The brown spot caused by Rhizoctonia solani is one of the most common diseases of creeping bentgrass (Agrostis stolonifera) lawns. Application of 2,3-butanediol (2,3-BD ) can induce plant disease response and improve disease resistance. In the present study, creeping bentgrass was inoculated with R. solani after treatment with 2,3-BD (250 μmol/L) and then the cell microstructure of leaves was observed using a paraffin sectioning method. It was found that the disease index of creeping bentgrass without 2,3-BD reached 70.45% after 15 days from inoculation. However, the disease index of creeping bentgrass treated with 2,3-BD was significantly lower, at 21.63% (P<0.05). Compared to leaves of pathogen-infected plants untreated with 2,3-BD, creeping bentgrass leaves with both pathogen inoculation and 2,3-BD treatment had a closely arranged cell structure, smaller cavities, more papillae, and increased upper and lower epidermal cell size. The size increase of upper and lower epidermal cells of 2,3-BD induced plants, compared to uninduced plants, was a factor of 1.19 and 1.26 times, respectively, and was most significant on day 7 after inoculation. The mesophyll cell size increase was the most significant on the 15th day (×1.30). The chloroplast density 1, 7, and 15 days after inoculation was, respectively, 1.25, 1.20, and 1.24 times that of uninduced plants (P<0.05). The size of vascular bundles and ducts did not change significantly at any time, however, the degree of lignification was higher and the cortical tissue became thicker in induced plants. In conclusion, 2, 3-BD can induce resistance of creeping bentgrass to brown spot disease through change in the cell structure. There was a positive correlation between the upper and lower epidermal cell size, the mesophyll cell size, and the number of chloroplasts per cell in creeping bentgrass leaves, and the disease resistance.