基于小尺度高原鼢鼠种群遗传结构研究

doi:10.11686/cyxb2017068

草业学报 ›› 2018, Vol. 27 ›› Issue (1): 123-130.DOI: 10.11686/cyxb2017068

基于小尺度高原鼢鼠种群遗传结构研究

刘丽, 王贵珍, 周延山, 楚彬, 马素洁, 姬程鹏, 田永亮, 花立民^*

甘肃农业大学草业学院,教育部草业生态系统重点实验室,甘肃农业大学-新西兰梅西大学草地多样性研究中心,甘肃兰州 730070

收稿日期:2017-03-01 修回日期:2017-07-03 出版日期:2018-01-20 发布日期:2018-01-20
通讯作者: E-mail:hualm@gsau.edu.cn
作者简介:刘丽(1988-),女,甘肃会宁人,硕士。E-mail:liull022013@126.com
基金资助:
国家重点研发计划(2017YFC0504800)和国家自然基金项目(31460635)资助

Small-scale genetic structural analysis of plateau zokor (Eospalax baileyi)

LIU Li, WANG Gui-zhen, ZHOU Yan-shan, CHU Bin, MA Su-jie, JI Cheng-peng, TIAN Yong-liang, HUA Li-min^*

College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education; Grassland Biodiversity Research Center of Gansu Agricultural University-New Zealand Massey University, Lanzhou 730070, China

Received:2017-03-01 Revised:2017-07-03 Online:2018-01-20 Published:2018-01-20

摘要/Abstract

摘要： 高原鼢鼠是青藏高原特有的地下啮齿动物,研究其种群遗传结构对于了解扩散、基因交流等方面具有重要意义。本研究利用微卫星分子标记技术,分析祁连山东段高寒草甸区小尺度下4个高原鼢鼠种群的遗传多样性和遗传结构特点。结果表明:1)4个种群有效等位基因(Ne)为83.6,种群平均观测杂合度(Ho)最高为0.32,平均期望杂合度(He)最高为0.50,平均多态信息含量(PIC)为0.40,遗传多态性处于中等水平。2)种群遗传结构推导分析结果为 4 个种群被分成 2 组,MYT(马营滩种群)为一组,XNG(下南泥沟种群)、MYR(马营河东边种群)、MYL(马营河西边种群)为一组。3)试验区高原鼢鼠种群间近交系数(F_ST)都大于0.05,种群间的基因流值(N_m)都小于1,特殊岛状栖息地限制了高原鼢鼠种群基因流,河流和公路可能对种群内和种群间个体交流有阻碍作用。

Abstract: The plateau zokor is a subterranean rodent that is one of the unique native animals living on the Tibetan Plateau. Research on the genetic structure of zokor will be useful to understand its patterns of dispersal and genetic exchange. In this study, we focused on the genetic structure of four plateau zokor populations in the alpine meadow in the eastern Qilian Mountains in northwest China. We used microsatellite marker technology to study the genetic diversity and structure of these populations. The effective alleles (Ne) value was 83.6 in the four different geographic populations. The highest average observed heterozygosity (Ho) value was 0.32, and the expected heterozygosity (He) value was 0.50. The average polymorphism information content (PIC) value was 0.40, indicating that plateau zokor have medium-level genetic diversity. A genetic structure analysis divided the four populations into two groups; one group containing the MYT (Mayingtan) population; and the other group containing XNG (Xianannigou), MYR, and MYL populations (west and east of the Maying river, respectively). The genetic distance (F_ST) values were greater than 0.05 and gene flow values were lower than 1 in the four geographical populations. These results indicated that the island-type habitat has limited genetic exchange among plateau zokor populations. Rivers and roads may have restricted the movement and exchange of individuals among and within populations.

刘丽, 王贵珍, 周延山, 楚彬, 马素洁, 姬程鹏, 田永亮, 花立民. 基于小尺度高原鼢鼠种群遗传结构研究[J]. 草业学报, 2018, 27(1): 123-130.

LIU Li, WANG Gui-zhen, ZHOU Yan-shan, CHU Bin, MA Su-jie, JI Cheng-peng, TIAN Yong-liang, HUA Li-min. Small-scale genetic structural analysis of plateau zokor (Eospalax baileyi)[J]. Acta Prataculturae Sinica, 2018, 27(1): 123-130.

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基于小尺度高原鼢鼠种群遗传结构研究

Small-scale genetic structural analysis of plateau zokor (Eospalax baileyi)

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