象草与皇竹草组合效应的研究

doi:10.11686/cyxb2017302

草业学报 ›› 2018, Vol. 27 ›› Issue (2): 135-145.DOI: 10.11686/cyxb2017302

象草与皇竹草组合效应的研究

吴征敏^{1, *}, 王志敬^{1, 2*, *}, 吴浩浩¹, 李政¹, 李文威¹, 庄桂锋¹, 尹福泉^{1, *}, 赵志辉¹

1.广东海洋大学农学院,广东湛江 524088;
2.衡阳德邦新金生物科技有限公司,湖南衡阳 421001

收稿日期:2017-07-10 修回日期:2017-09-12 出版日期:2018-02-20 发布日期:2018-02-20
通讯作者: yinfuquan01@163.com
作者简介:吴征敏(1989-),男,安徽萧县人,硕士。E-mail:wuzm1314@126.com。王志敬(1990-),男,安徽定远人,硕士。E-mail:zhijingw@126.com。**共同第一作者These authors contributed equally to this work.
基金资助:
舍饲条件下雷州山羊养殖关键技术研究与示范(2015A03029),舍饲雷州山羊全混合日粮(TMR)配方研制及应用(2016LM3189)和广东省大学生创新实验项目(524000087206; CXXL2016048; 201610566061)资助

Study on the combination effect of Pennisetum purpureum and Pennisetum sinese

WU Zheng-min^{1, **}, WANG Zhi-jing^{1, 2, **}, WU Hao-hao¹, LI Zheng¹, LI Wen-wei¹, ZHUANG Gui-feng¹, YIN Fu-quan^{1, *}, ZHAO Zhi-hui¹

1.College of Agricultural, Guangdong Ocean University, Zhanjiang 524088, China;
2.Hengyang Debon Xinjin Bio-Tech CO.,LTD, Hengyang 421001, China

Received:2017-07-10 Revised:2017-09-12 Online:2018-02-20 Published:2018-02-20

摘要/Abstract

摘要： 本试验旨在通过体外产气法研究象草与皇竹草不同比例组合对山羊瘤胃降解率的影响,为南方粗饲料资源的科学应用提供理论依据和数据支持。试验将象草与皇竹草按干物质比为100∶0,75∶25,50∶50,25∶75,0∶100分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,通过体外产气法培养4、8、12、24和48 h,每个时间点3个重复,培养结束后终止发酵并测定各时间点发酵液pH、氨态氮(NH₃-N)浓度、总挥发性脂肪酸(TVFA)浓度和发酵滤渣的干物质(DM)、粗蛋白质(CP)和中性洗涤纤维(NDF)含量。结果表明:1)象草和皇竹草组合影响了瘤胃pH和NH₃-N浓度,随皇竹草添加比例的增加,pH逐渐降低,显著低于单一象草组(P<0.05);NH₃-N浓度呈先降低后升高的趋势(P>0.05),数值在正常变化范围内,有利于瘤胃发酵;随皇竹草比例的增加,瘤胃TVFA浓度逐渐增加,在发酵8~48 h组Ⅲ、Ⅳ和Ⅴ显著高于单一象草组(P<0.05)。2)随皇竹草比例增加,DM、NDF和CP降解率增高,显著高于单一象草组(P<0.05),添加75%皇竹草组较其他组高,较为理想。3)象草和皇竹草组合产生了正组合效应,添加50%皇竹草组DM降解率组合效应达到最大值、NDF降解率组合效应均为正值;添加75%皇竹草组在发酵12 h NDF降解率组合效应达到最大值(P<0.05);在发酵4和48 h添加皇竹草组CP降解率组合效应为正。综合得出,象草和皇竹草组合能提高DM、NDF和CP降解率,产生正组合效应;象草和皇竹草以25∶75组合效果较好。

Abstract: The study investigated the effects of the combination of Pennisetum purpureum and Pennisetum sinese in different proportion on goats rumen degradation rate by using in vitro gas production technique so as to provide theoretical basis and data support for scientific application of roughage resources in South China. According to the ratio of dry substance, P. purpureum and P. sinese were mixed in the ration of 100∶0, 75∶25, 50∶50, 25∶75, 0∶100 and divided into group Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ respectively, which were cultured for 4, 8, 12, 24 and 48 hours by using in vitro gas production respectively. Three replicates in each time point. We stopped fermentation and determined liquid pH, NH₃-N concentration and the amounts of DM (dry matter), CP (dry matter) and NDF (neutral detergent fiber) in fermentation residue. The results showed as follows: 1) Combinations of P. purpureum and P. sinese influenced rumen pH and NH₃-N concentration while pH decreased significantly (P<0.05) as the increase of the proportion of P. sinese, which was significantly lower than single P. purpureum group (P<0.05); The concentration of NH₃-N firstly decreased and then increased (P>0.05) with their normal fluctuation range, which was beneficial to rumen fermentation. Rumen TVFA (total volatile fatty acids) concentration increased gradually with the increase of P. sinese ratio. Ⅲ, Ⅳ and Ⅴ was significantly higher than that of single P. purpureum group (P<0.05) in 8-48 h. 2) The degradation rate of DM, NDF and CP increased significantly with the increase of the proportion of P. sinese, which was significantly higher than single P. purpureum group (P<0.05), and 75% P. sinese group was higher than those of the other groups as the better proportion. 3) The combined effects of P. purpureum and P. sinese were positive. The maximum combination effect (P<0.05) of DM degradation rate and positive combination effect of NDF degradation rate was obtained in 50% P. sinese group; The maximum combination effects (P<0.05) on the degradation rate of NDF in 75% P. sinese group was obtained at 12 hours; The combined effects on CP degradation rate were positive in P. sinese group at 4 and 48 h. We concluded that the combination of P. purpureum and P. sinese may improve the degradation rate of DM, NDF, CP and positive combination effect while better proportion of P. purpureum and P. sinese was 25∶75.

吴征敏, 王志敬, 吴浩浩, 李政, 李文威, 庄桂锋, 尹福泉, 赵志辉. 象草与皇竹草组合效应的研究[J]. 草业学报, 2018, 27(2): 135-145.

WU Zheng-min, WANG Zhi-jing, WU Hao-hao, LI Zheng, LI Wen-wei, ZHUANG Gui-feng, YIN Fu-quan, ZHAO Zhi-hui. Study on the combination effect of Pennisetum purpureum and Pennisetum sinese[J]. Acta Prataculturae Sinica, 2018, 27(2): 135-145.

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象草与皇竹草组合效应的研究

Study on the combination effect of Pennisetum purpureum and Pennisetum sinese

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