利用CRISPR/Cas9技术敲除拟南芥转录因子MYB 40的两种可变剪接体

doi:10.11686/cyxb2018099

草业学报 ›› 2019, Vol. 28 ›› Issue (1): 120-127.DOI: 10.11686/cyxb2018099

利用CRISPR/Cas9技术敲除拟南芥转录因子MYB 40的两种可变剪接体

李孟湛, 尹红菊^*, 李丁丁, 刘亚琪, 王锁民

兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2018-02-06 出版日期:2019-01-20 发布日期:2019-01-20
通讯作者: *E-mail: yinhj@lzu.edu.cn
作者简介:李孟湛(1992-),女,山东菏泽人,在读博士。E-mail: limzh2015@lzu.edu.cn
基金资助:
国家自然科学基金青年基金项目(31601992)资助

Knock out of two splice variants of MYB 40 using the gene-editing technique CRISPR/Cas9

LI Meng-zhan, YIN Hong-ju^*, LI Ding-ding, LIU Ya-qi, WANG Suo-min

State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2018-02-06 Online:2019-01-20 Published:2019-01-20
Contact: *E-mail: yinhj@lzu.edu.cn

摘要/Abstract

摘要： MYB家族转录因子在植物抵抗非生物胁迫过程中发挥着重要功能。前期研究发现强旱生植物霸王转录因子ZxMYB315参与到植物抗逆过程中,其在拟南芥中的同源基因MYB40的两种不同可变剪接体MYB40.1和MYB40.2对盐胁迫均有明显响应,但其在植物抗逆过程中的功能还未见报道。分析了在盐处理条件下MYB40.1及MYB40.2的表达模式,并利用CRISPR/Cas9技术,获得了2个MYB40.1被编辑株系,及3个MYB40.1和MYB40.2被同时编辑株系,为揭示MYB40这两种可变剪接体在拟南芥响应逆境胁迫过程中的功能和分子机理奠定了基础。

关键词: 转录因子, MYB40.1, MYB40.2, 抗逆, CRISPR/Cas9技术

Abstract: MYB transcription factors play important roles in various abiotic stress signaling pathways. In a previous study, we found that ZxMYB315 from Zygophyllum xanthoxylum is involved in plant responses to salt and drought stresses. In addition, the ortholog of ZxMYB315 in Arabidopsis, MYB40, has two splice variants, MYB40.1 and MYB40.2. Expression levels of both MYB40.1 and MYB40.2 are affected by salt treatment. However, the functions of these changes in response to abiotic stress are still unclear. In this study, we further analyzed the expression patterns of MYB40.1 and MYB40.2 under salt treatment. In particular, two heterozygous mutations for MYB40.1 and three heterozygous mutations for both MYB40.1 and MYB40.2 were generated using the gene-editing technique CRISPR/Cas9. These mutants now provide a foundation for studying functions and molecular mechanisms of MYB40 in various abiotic stress signaling pathways.

Key words: transcription factor, MYB40.1, MYB40.2, abiotic stress tolerance, CRISPR/Cas9

李孟湛, 尹红菊, 李丁丁, 刘亚琪, 王锁民. 利用CRISPR/Cas9技术敲除拟南芥转录因子MYB 40的两种可变剪接体[J]. 草业学报, 2019, 28(1): 120-127.

LI Meng-zhan, YIN Hong-ju, LI Ding-ding, LIU Ya-qi, WANG Suo-min. Knock out of two splice variants of MYB 40 using the gene-editing technique CRISPR/Cas9[J]. Acta Prataculturae Sinica, 2019, 28(1): 120-127.

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利用CRISPR/Cas9技术敲除拟南芥转录因子MYB 40的两种可变剪接体

Knock out of two splice variants of MYB 40 using the gene-editing technique CRISPR/Cas9

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