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草业学报 ›› 2019, Vol. 28 ›› Issue (9): 123-134.DOI: 10.11686/cyxb2018602

• 研究论文 • 上一篇    下一篇

外源纤维素酶对发酵全混合日粮营养价值、发酵品质和酶活性的动态影响

陈光吉1, 吴佳海1, *, 尚以顺1, 张蓉1, 何廷章2, 吴彦3, 娄芬4, 熊俊4, 甘小波4, 陈荫琼4, 王普昶1, 韩永芬5, 李世歌1, 舒建虹1   

  1. 1.贵州省农业科学院草业研究所,贵州 贵阳 550006;
    2.绥阳县枧坝镇农业服务中心,贵州 绥阳 563307;
    3.绥阳县草地工作站,贵州 绥阳 563300;
    4.大方县农业农村局,贵州 大方 551600;
    5.贵州金农富平生态农牧科技有限公司,贵州 贵阳 550006
  • 收稿日期:2018-09-13 修回日期:2019-03-11 出版日期:2019-09-20 发布日期:2019-09-20
  • 通讯作者: *E-mail: 1848266168@qq.com
  • 作者简介:陈光吉(1990-),男,贵州遵义人,助理研究员,硕士。E-mail: cgjgz09@126.com
  • 基金资助:
    贵州省科技计划项目(黔科合成果[2017]4117),贵州省农业科学院科技创新项目(黔农科院科技创新[2017]01号),贵州省科技计划项目 (黔科合支撑[2017]2591),国家重点研发计划(2017YFD0502101-3),贵州省农业科学院农业科技改革与创新服务园区项目,贵州省优秀青年科技人才培养对象专项(黔科合人字(2015)02号),贵州省科学技术基金(黔科合J字 [2015]2080号),科技创新人才团队建设项目(黔科合平台人才[2016]5617),贵州省科技计划项目(黔科合服企[2018]4001)和贵州省科技计划项目(黔科合人才[2016]5713)资助

Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration

CHEN Guang-ji1, WU Jia-hai1,*, SHANG Yi-shun1, ZHANG Rong1, HE Ting-zhang2, WU Yan3, LOU Fen4, XIONG Jun4, GAN Xiao-bo4, CHEN Yin-qiong4, WANG Pu-chang1, HAN Yong-fen5, LI Shi-ge1, SHU Jian-hong1   

  1. 1.Guizhou Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2.Agricultural Service Center, Jian Ba Town, Suiyang County, Suiyang 563307, China;
    3.Grassland Workstation of Suiyang County, Suiyang 563300, China;
    4.Agriculture and Rural Bureau of Dafang County, Dafang 551600, China;
    5.Guizhou Jinnong Fuping Ecological Agriculture and Animal Husbandry Technology Co., Ltd., Guiyang 550006, China
  • Received:2018-09-13 Revised:2019-03-11 Online:2019-09-20 Published:2019-09-20
  • Contact: *E-mail: 1848266168@qq.com

摘要: 本试验旨在探究外源纤维素酶(EFE)添加到发酵全混合日粮(FTMR)中对其营养价值、发酵品质和酶活性的影响,并找出适宜添加水平。采用完全随机试验设计,设置5个纤维素酶添加水平(0、0.4、0.8、1.6、3.2 g·kg-1 DM),分别测定底物发酵的第7、14、35、49、70天的概略养分、可溶性碳水化物(WSC)、发酵参数和4种酶活力。结果表明,1)纵向看,随发酵时间的延长,底物的干物质(DM)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和可溶性碳水化合物含量,以及4种酶活力均呈显著下降趋势(P<0.05),而乙酸含量则呈显著上升趋势(P<0.05)。2)横向看,随着EFE添加量的提高,发酵一定时间后, DM、NDF、ADF和WSC含量以及pH值、氨态氮含量、羧基肽酶活力和酸性蛋白酶活力呈显著下降趋势(P<0.05),而乙酸含量、纤维素酶活力、和木聚糖活力呈上升趋势(P<0.05),发酵第35天后趋于稳定,0.8和1.6 g·kg-1 EFE添加组表现出较低的pH值、NDF含量和ADF含量(P<0.05),以及较高的纤维素酶和木聚糖酶活力(P<0.05);由此,本试验发现,添加EFE可促进FTMR中纤维类物质的降解、有机酸的积累和自身纤维类物质的酶活力,抑制羧基肽酶活力和酸性蛋白酶活力,减少蛋白质的损失,且存在添加剂量效应,适宜添加量为0.8~1.6 g·kg-1 DM。

关键词: FTMR, 外源纤维素酶, 酶活力, 动态影响

Abstract: The objective of this study was to determine the effect of exogenous fibrolytic enzyme (EFE) supplementation in fermentated total mixed ration (FTMR) on the nutritional value, fermentation quality and enzyme activities, and to find out the optimal level of EFE addition. A completely randomized experimental design was adopted, and 5 levels of EFE (0, 0.4, 0.8, 1.6 and 3.2 g·kg-1 dry matter) were added to FTMR as the 5 experimental treatments. At 7, 14, 35, 49, 70 days after initiation of fermentation, major nutrients, water soluble carbohydrate (WSC), fermentation parameters and activities of four enzymes were determined. It was found: 1) With increased fermentation time, the dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and WSC contents and the activities of the four enzymes monitored showed a decrease (P<0.05), while acetic acid accumulated with time (P<0.05). 2) With increased EFE supplementation, the DM, NDF, ADF and WSC contents, pH value, ammonia nitrogen content, and the activities of carboxyl peptidase and acid protease decreased (P<0.05) until day 35 and then stabilised, while the acetic acid content, and cellulase and xylanase activities increased and stabilized over the same time period (P<0.05). At day 35, the EFE supplementation treatments of 0.8 and 1.6 g·kg-1 showed lower pH values, NDF and ADF contents, and higher activity of cellulase and xylanase (P<0.05). It was found that the addition of EFE promoted fiber degradation and the accumulation of organic acids through enzymatic activity, inhibited the activities of carboxypeptidase and acidic protease, and reduced the loss of protein in FTMR. The dose effect was additive and the optimum EFE dose was 0.8-1.6 g·kg-1 DM.

Key words: FTMR, exogenous fibrolytic enzymes, enzyme activity, dynamic effects