酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响

doi:10.11686/cyxb2019466

草业学报 ›› 2020, Vol. 29 ›› Issue (8): 134-142.DOI: 10.11686/cyxb2019466

酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响

张利霞¹, 常青山^{2, *}, 薛娴¹, 刘伟¹, 张巧明², 陈苏丹², 郑轶琦², 李景林², 陈婉东², 李大钊³

1. 河南科技大学农学院,河南洛阳 471000;
2. 河南科技大学林学院,河南洛阳 471000;
3. 河南科技大学食品与生物工程学院,河南洛阳 471000

收稿日期:2019-10-30 修回日期:2019-12-16 出版日期:2020-08-20 发布日期:2020-08-20
通讯作者: *. E-mail: hkdcqs@126.com
作者简介:张利霞(1982-),女,河南荥阳人,讲师,博士。E-mail: hkdzlx@126.com
基金资助:
国家自然科学基金项目(31870093,31800096),河南省自然科学基金项目(182300410082,182300410050,182300410017),河南省科技攻关计划项目(182102110401),河南科技大学培育基金(4024-13350073)和河南科技大学大学生研究训练计划(SRTP)项目(2018162,2019422)资助

Effects of acid stress on chlorophyll fluorescence characteristics and root antioxidant activity of Prunella vulgaris

ZHANG Li-xia¹, CHANG Qing-shan^{2, *}, XUE Xian¹, LIU Wei¹, ZHANG Qiao-ming², CHEN Su-dan², ZHENG Yi-qi², LI Jing-lin¹, CHEN Wan-dong¹, LI Da-zhao³

1. College of Agriculture, Henan University of Science and Technology, Luoyang 471000, China;
2. College of Forestry, Henan University of Science and Technology, Luoyang 471000, China;
3. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China

Received:2019-10-30 Revised:2019-12-16 Online:2020-08-20 Published:2020-08-20

摘要/Abstract

摘要： 为了阐明夏枯草幼苗对酸性环境的耐受性,采用不同pH值(pH=4.5、4.0、3.5、3.0)的Hoagland营养液模拟酸胁迫环境,研究酸胁迫对夏枯草幼苗叶绿素荧光特性及根系抗氧化酶活性的影响。结果表明,随着pH值下降,代表供体与受体侧参数的K点相对可变荧光(W_K)、J点相对可变荧光(V_J)和电子受体QA-被还原的最大速率(dV/dt_o)逐渐升高,光系统Ⅱ(PSⅡ)电子传递的量子效率(φE_o)降低;表示单位反应中心吸收(ABS/RC)、捕获(TR_o/RC)、热耗散(DI_o/RC)的能量逐渐增加;用于电子传递的能量(ET_o/RC)逐渐下降;表示PSⅡ的最大光化学效率(F_v/F_m)、吸收光能为基础的光合性能指数(PI_abs)、表征光系统Ⅰ(PSⅠ)的最大氧化还原活性(ΔI/I_o)及PSⅡ与PSⅠ协调性(Φ_PSⅠ/PSⅡ)随酸胁迫程度增加逐渐下降。夏枯草根系超氧化物歧化酶(SOD)与过氧化物酶(POD)活性呈下降趋势,而根系抗坏血酸过氧化物酶(APX)活性先升高后降低;夏枯草叶片可溶性糖、脯氨酸含量逐渐上升。酸胁迫下,夏枯草PSⅡ反应中心受损,电子传递受阻,光系统性能及PSⅡ与PSⅠ的协调性降低,根系SOD与POD活性受到抑制,在pH 3.0处理下表现较为明显。夏枯草通过提高单位反应中心的热耗散能力来减少过剩光能对叶片光合机构的破坏,通过提高根系APX抗氧化酶及叶片渗透调节物质含量来减少酸胁迫带来的伤害。

关键词: 夏枯草, 光系统, 酸胁迫, 抗氧化酶, 叶绿素荧光

Abstract: This research aimed to elucidate the tolerance of Prunella vulgaris seedlings to acid environments. Hoagland nutrient solutions with a range of pH values (pH=4.5, 4.0, 3.5, 3.0) were used to simulate environmental acid stress, and the effects of acid stress on chlorophyll fluorescence characteristics and root antioxidant activity were studied. It was found that at lower pH the quantum efficiency of PSⅡ electron transfer (φE_o) in leaves of P. vulgaris decreased, while a number of other fluorescence parameters increased, including: the variable fluorescence F_k to the amplitude F_j-F_o (W_K), relative variable fluorescence at J step (V_J), QA- maximum rate of reduction (dV/dt_o). The absorption flux per reaction center (ABS/RC), trapped energy flux per RC (TR_o/RC) and dissipated energy flux per RC (DI_o/RC) increased, but electron transport flux per RC (ET_o/RC) decreased gradually. The maximum quantum yield of PSⅡ (F_v/F_m), the photosynthetic index based on absorption basis (PI_abs), the maximum PSⅠ redox activity (ΔI/I_o), and the coordination of photosystem between Ⅰ and Ⅱ (Φ_PSⅠ/PSⅡ) all decreased under acid stress. The activity of superoxide dismutase (SOD) and peroxidase (POD) in roots decreased, but the activity of ascorbic acid peroxidase (APX) in roots increased on initial exposure to acid stress and then decreased. The soluble sugar and proline concentrations in leaves of P. vulgaris increased with the decrease in pH value. In summary, the PSⅡ reaction center of P. vulgaris was damaged, the electron transfer was blocked, the photosystem performance and the coordination of photosystem between Ⅰ and Ⅱ were reduced under acid stress, and the activities of SOD and POD in roots were inhibited, the latter being most obvious in the pH 3.0 treatment. P. vulgaris was able to reduce the damage to the photosynthetic mechanism by increasing the heat dissipation capacity of unit reaction center, and reduce the damage caused by acid stress by increasing the APX activity in roots and the concentration of osmolytes in leaves.

Key words: Prunella vulgaris, photosystem, acid stress, antioxidant enzymes, chlorophyll fluorescence

张利霞, 常青山, 薛娴, 刘伟, 张巧明, 陈苏丹, 郑轶琦, 李景林, 陈婉东, 李大钊. 酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响[J]. 草业学报, 2020, 29(8): 134-142.

ZHANG Li-xia, CHANG Qing-shan, XUE Xian, LIU Wei, ZHANG Qiao-ming, CHEN Su-dan, ZHENG Yi-qi, LI Jing-lin, CHEN Wan-dong, LI Da-zhao. Effects of acid stress on chlorophyll fluorescence characteristics and root antioxidant activity of Prunella vulgaris[J]. Acta Prataculturae Sinica, 2020, 29(8): 134-142.

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酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响

Effects of acid stress on chlorophyll fluorescence characteristics and root antioxidant activity of Prunella vulgaris

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