甘肃中部地区禾谷镰孢的变异研究

草业学报 ›› 2009, Vol. 18 ›› Issue (1): 118-124.

甘肃中部地区禾谷镰孢的变异研究

李金花^1,2, 纪莉景³, 柴兆祥^2*, Knight T E⁴, Burgess L W⁴

1.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070;
2.甘肃农业大学草业学院植物病理学系,甘肃兰州730070;
3.河北农林科学院植保所,河北保定071001;
4.澳大利亚悉尼大学农业、食品和自然资源学院镰刀菌研究实验室,悉尼2006

收稿日期:2007-12-06 出版日期:2009-01-25 发布日期:2009-02-20
作者简介:李金花(1972-),女,甘肃秦安人,副教授。 E-mail: ljh@gsau.edu.cn
基金资助:
澳大利亚ATSE/Crawford 基金,甘肃省科技厅(甘科计[2001]21号)和甘肃省教育厅项目(032208)资助。

A study on the variance of Fusarium graminearum sampled from central Gansu

LI Jin-hua^1,2, JI Li-jing³, CHAI Zhao-xiang², Knight T E⁴, Burgess L W⁴

1.Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China;
2.Department of Plant Pathology, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China;
3.Institute of Plant Protection, Hebei Academy of Agricultural and
Forestry Science, Baoding 071001, China;
4.Fusarium Research Laboratory, Faculty of
Agriculture, Food and Natural Resources, University of Sydney,
NSW, Sydney 2006, Australia

Received:2007-12-06 Online:2009-01-25 Published:2009-02-20

摘要/Abstract

摘要： 为掌握禾谷镰孢在甘肃中部地区的分布及变异情况,从根部表现有坏死和叶鞘发褐的小麦幼苗的不同部位、小麦地土壤及玉米籽粒、玉米茎秆上分离禾谷镰孢,并以形态学为基础,参照Nelson分类系统进行鉴定。结果表明,在分离到的43个镰刀菌菌株中,有14个菌株经鉴定为禾谷镰孢,均从玉米茎秆上分离到,小麦根部、小麦叶鞘、小麦地土壤、玉米籽粒中分离到的镰刀菌中未见禾谷镰孢。将禾谷镰孢在特定条件下培养后,发现14个禾谷镰孢菌株产生子囊壳的数量不同,为2～90个。在以Fg16为引物的PCR反应中,随机选取的11个禾谷镰孢菌株都产生0.41kb的PCR产物,而6个对照菌株都产生0.50kb的片段,证明引物Fg16可以区分禾谷镰孢菌株群体的遗传多态性。以Tri13为引物的PCR反应显示,11个禾谷镰孢菌株以及3个中国对照菌株都产生脱氧雪腐镰刀菌烯醇(DON)毒素,而3个澳大利亚对照菌株产生雪腐镰刀菌烯醇(NIV)毒素。

Abstract: The occurrence of Fusarium graminearum on Triticum aestivum and Zea mays, in the middle areas of Gansu Province, China was assessed by isolation of Fusarium species from T. aestivum roots with brown rot symptoms, from T. aestivum sheaths, T. aestivum soil, Z. mays kernels, and Z. mays stalks. Fusarium isolates were identified by morphology according to Nelson’s system. Fourteen of 43 Fusarium isolates were F. graminearum. It was isolated only from corn stalks. These 14 isolates produced between 2 and 90 perithecia each. PCR using the Fg16 primer set resulted in a product of 0.41 kb from all 14 isolates and of 0.50 kb from the 6 reference isolates. It successfully differentiated between the F. graminearum isolates. PCR reactions using the Tri13 primer showed that all 14 isolates and the three Chinese reference cultures produced DON while the three Australian reference isolates produced fragments of 415 bp showing that they produced NIV.

中图分类号:

S435.121.4

李金花, 纪莉景, 柴兆祥, Knight T E, Burgess L W. 甘肃中部地区禾谷镰孢的变异研究[J]. 草业学报, 2009, 18(1): 118-124.

LI Jin-hua, JI Li-jing, CHAI Zhao-xiang, Knight T E, Burgess L W. A study on the variance of Fusarium graminearum sampled from central Gansu[J]. Acta Prataculturae Sinica, 2009, 18(1): 118-124.

参考文献

[1] Rubella S G, Kistler H C. Heading for disaster: Fusarium graminearum on cereal crops[J]. Molecular Plant Pathology, 2004, 5(6): 515-525.
[2] Sutton J C. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum[J]. Canadian Journal of Plant Pathology, 1982, 4: 195-209.
[3] Parry D W, Jenkinson P, Mclead L. Fusarium ear blight (scab) in small grain cereals-a review[J]. Plant Pathology, 1995, 44: 207-238.
[4] Blaney B J, Dodman R L. Production of zearalenone, deoxynivalenol, nivalenol, and acetylated derivatives by Australian isolates of Fusarium graminearum and F. pseudograminearum in relation to source and culturing conditions[J]. Australian Journal of Agricultural Research, 2002, 53: 1317-1326.
[5] O’Donnell K, Kistler H C, Tacke B K, et al. Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab[A]. Proceedings of the National Academy of Sciences of the United States of America[C]. Washington:University of Washington Press, 2000.
[6] Williams K C, Blaney B J. Effect of the mycotoxins, nivalenol and zearalenone, in maize naturally infected with Fusarium graminearum on the performance of growing and pregnant pigs[J]. Australian Journal of Agricultural Research, 1994, 45: 1265-1279.
[7] Dusabenyagasani M, Dostaler D, Hamelin R C. Genetic diversity among Fusarium graminearum strains from Ontario and Quebec[J]. Canadian Journal of Plant Pathology, 1999, 21: 308-314.
[8] Jennings P, Coates M E, Walsh K, et al. Determinaiton of deoxynivalenol and nivalenol-producing chemotypes of Fusarium graminearum isolated from wheat crops in England and Wales[J]. Plant Pathology, 2004, 53: 643-652.
[9] Gale L R, Chen L F, Hernick C A, et al. Population analysis of Fusarium graminearum from wheat fields in eastern China[J]. Phytopathology, 2002, 92: 1315-1322.
[10] 胡光荣, 赵纯森, 廖玉才. 我国小麦禾谷镰刀菌(Fusarium graminearum)营养体亲合性与致病性研究[J].植物病理学报,2003,33(4):379-380.
[11] 赵纯森,马星霞,武爱波,等.禾谷镰刀菌培养性状与致病力的相关性分析[J]. 华中农业大学学报,2005, 24(3):254-257.
[12] Wang Y Z. Epidemiology and management of wheat scab in China[A]. Fusarium Head Scab: Global Status and Future Prospects[M]. Mexico: Mexico D F, 1997.
[13] Tio M, Burgess L W, Nelson P E, et al. Techniques for the isolation, culture and preservation of the Fusaria[J]. Australian Plant Pathology Society Newsletter, 1977, 6: 11-13.
[14] Fisher N L, Burgess L W, Tousson T A, et al. Carnation leaves as a substrate for preserving cultures of Fusarium species[J]. Phytopathology, 1982, 72: 151-153.
[15] Burgess L W, Sumerell B A, Bullock S, et al. Laboratory Manual for Fusarium Research(3rd ed)[M]. Sydney: Sydney University Press, 1994.
[16] Knight T. Genetic and phenotypic variation between two geographically isolated populations of Fusarium graminearum in Australia(0228578)[R]. Sydney: Sydney University Press, 2005.
[17] Smith W J, Brett A S, Gunn L, et al. Molecular Techniques for the Study of Soilborn Fungal Plant Panthology[M]. Sydney: Sydney University Press, 2003.
[18] Chandler E A, Duncan R S, Thomsett M A, et al. Development of PCR assays to Tri7 and Tri13 and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis[J]. Physiological and Molecular Plant Pathology, 2003, 62: 355-367.
[19] Walker S L, Leath S, Hagler W M, et al. Variation among isolates of Fusarium graminearum associated with Fusarium head blight in North Carolina[J]. Plant Disease, 2001, 85: 404-410.
[20] Cook R J. Fusarium: Diseases, Biology, and Taxonomy[M]. University Park, PA, Pennsylvania State University Press, 1981.
[21] Carter J P, Rezanoor H N, Desjardins A E, et al. Variation in Fusarium graminearum isolates from Nepal associated with their host of origin[J]. Plant Pathology, 2000, 49: 452-460.
[22] Carter J P, Rezanoor H N, Holden D, et al. Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum[J]. European Journal of Plant Pathology, 2002, 108(6): 573-583.
[23] Desjardins A E, Plattner R D. Diverse traits for pathogen fitness in Gibberella zeae[J]. Canadian Journal of Plant Pathology, 2003, 25: 21-27.
[24] Zeller K A, Bowden R L, Leslie J F. Population differentiation and recombination in wheat scab populations of Gibberella zeae from the United States[J]. Molecular Ecology, 2004, 13: 563-571.
[25] 曲涛,南志标.作物和牧草对干旱胁迫的响应及机理研究进展[J].草业学报,2008,17(2):126-135.
[26] 王生荣,郑华平,何冬云,等. 天祝高寒草原草地植物黑粉病调查研究[J].草业学报,2008,17(1):48-51.
[27] 张礼军,张恩和,郭丽琢,等. 水肥耦合对小麦/玉米系统根系分布及吸收活力的调控[J].草业学报,2005,14(2):102-108.
[28] 陈本建. 甘肃省豆科牧草资源及植物区系分析[J].草业科学,2008,25(4):42-45.
[29] 刘生瑞,常生华,侯扶江. 甘肃省退耕还林还草区划刍议[J].草业科学,2008,25(1):5-9