结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记

草业学报 ›› 2009, Vol. 18 ›› Issue (1): 72-79.

结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记

薛丹丹^1,2,郭海林¹,郑轶琦¹,陈宣¹,刘建秀^1*

1.江苏省中国科学院植物研究所,江苏南京210014;
2.南京农业大学园艺学院,江苏南京210095

收稿日期:2008-03-26 出版日期:2009-01-25 发布日期:2009-02-20
作者简介:薛丹丹(1982-),女,河南济源人,在读硕士。E-mail: yz129@163.com
基金资助:
江苏省科技攻关项目(BE2006339),国家科技支撑项目(2006BAD01A19-3),国家自然科学基金项目(30571307)和江苏省高技术项目(BG2006320)资助。

Hybrid identification of progenies of Zoysia crosses by SRAP marker

XUE Dan-dan^1,2, GUO Hai-lin¹, ZHENG Yi-qi¹, CHEN Xuan¹, LIU Jian-xiu¹

1.Insititute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;
2. College of Horticulture,Nanjing Agricultural University, Nanjing 210095, China

Received:2008-03-26 Online:2009-01-25 Published:2009-02-20

摘要/Abstract

摘要： SRAP标记是基于PCR技术的一种新型分子标记。本研究通过SRAP分子标记技术对结缕草属植物种间杂交的5个组合37份杂交后代进行了真假杂种的鉴定,以为育种工作进一步开展奠定基础。首先筛选出在父本中能扩增出,而在母本中没有的特征带的引物,共筛选出9对引物对后代进行真实性鉴定。把父母本和后代一起进行PCR扩增、电泳,根据后代中是否能扩增出父本特征带而进行鉴定。结果表明,37个杂交后代中有32个后代具有父本特征带,被鉴定为真杂种。同时,从杂种扩增的谱带来看,条带清晰、稳定且后代出现了丰富的变异,主要表现为新谱带的出现或某些谱带的消失。据此认为,SRAP标记可以有效地对结缕草属杂交后代进行真实性鉴定和遗传分析,为杂交育种提供了理论依据。

Abstract: Sequence-related Amplified Polymorphism (SRAP) is a new molecular marker technique developed on the basis of PCR. The authenticity of 37 progenies of 5 Zoysia grass cross-combinations were identified by SRAP marker to select out the true hybrids for further study. First, primers were selected to bands specific for the male parent but absent from the female, then 9 primers were selected and applied to identify the hybrids. The authenticity of the progenies was identified by whether or not they had the male parent specific bands. There were 32 progenies with these specific bands, which were therefore identified as true hybrids. The amplified bands of SRAP were clear and stable, and there was considerable variation in the hybrids, including some with new bands and some without the parent bands. The results suggest that SRAP markers could be used as effective molecular markers for the authenticity, identification and genetic analysis of the progenies of Zoysia grass

中图分类号:

薛丹丹,郭海林,郑轶琦,陈宣,刘建秀. 结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记[J]. 草业学报, 2009, 18(1): 72-79.

XUE Dan-dan, GUO Hai-lin, ZHENG Yi-qi, CHEN Xuan, LIU Jian-xiu. Hybrid identification of progenies of Zoysia crosses by SRAP marker[J]. Acta Prataculturae Sinica, 2009, 18(1): 72-79.

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