氮肥对苦参生长和生物总碱的效应

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 159-164.

氮肥对苦参生长和生物总碱的效应

纪瑛¹,张庆霞¹,蔺海明^2*,蔡伟¹

1.甘肃农业职业技术学院,甘肃兰州730020;
2.甘肃农业大学农学院,甘肃兰州 730070

收稿日期:2008-07-15 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:纪瑛(1963-),女,甘肃定西人,副教授,博士。E-mail:lzjiying2005@163.com
基金资助:
甘肃省科技攻关项目(2GS064-A43-019-08)资助。

Effect of nitrogen on growth and total alkaloids of Sophora flavescens

JI Ying¹, ZHANG Qing-xia¹, LIN Hai-ming², CAI Wei¹

1.Gansu Agricultural College, Lanzhou 730020, China;
2.College of Agronomy, Gansu
Agricultural University, Lanzhou 730070, China

Received:2008-07-15 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 对苦参进行驯化栽培是解决其供需矛盾的主要途径,也是保护生态环境最有效的措施。本试验研究氮肥对苦参生长、生物总碱含量和生物总碱产量的效应。采用盆栽试验,施纯氮量设0,50,100,150和200 mg/kg五个水平,完全随机设计。结果表明,施用氮肥对苦参生长以及苦参总碱含量均有显著促进效应。各施氮处理根干重均与对照差异显著,当施纯氮150 mg/kg时根干重最高,比对照增加42％;根直径随施氮量提高呈增加趋势,当施纯氮150 mg/kg时根直径最大,比对照增加21.1％;叶绿素总含量在施纯氮100 mg/kg时最高,比对照增加33.8％;苦参总碱含量在施纯氮100 mg/kg时最高,比对照增加35.7％;单株苦参总碱产量在施纯氮150 mg/kg时最高,较对照增加90.5％。氮对根干重增加效应主要是由于根直径增加引起根体积增加所致,苦参叶片中叶绿素含量随施氮量增加有利于苦参总碱含量提高。

Abstract: The effect of nitrogen on growth, total alkaloids concentration and total alkaloids yield of Sophora flavescens was studied. Four levels of N (50, 100, 150, 200 mg/kg) were applied using urea (N 46%) in a pot experiment. The control was no N. Plant height, root diameter, root dry weight, aerial dry weight, Chl concentration and total alkaloids concentration increased relative to the amount of N applied. The root dry weight was 26.7 g/plant at 150 mg/kg N applied, an increase of 42% compared with the control. Root dry weight/root fresh weight and root dry weight/total plant dry weight decreased with amount of N applied. Nitrogen application had significant positive effect on root diameter. The root diameter was highest at 150 mg/kg N applied, an increase of 21.1% compared with the control. The percentage of xylem diameter increased with N applied while the percentage of root bark depth decreased. Chl concentration and total alkaloids concentration increased with N applied. Chl concentration was highest at 100 mg/kg N applied, an increase of 33.8% compared with the control. Total alkaloids concentration (4.4%) was also highest at 100 mg/kg N applied, an increase of 35.7% compared with the control. Total alkaloids concentration increased by improving Chl concentration. The total alkaloids yield per plant (1.18 g/plant) was highest at 150 mg/kg N applied, an increase of 90.5% compared with the control.

中图分类号:

纪瑛,张庆霞,蔺海明,蔡伟. 氮肥对苦参生长和生物总碱的效应[J]. 草业学报, 2009, 18(3): 159-164.

JI Ying, ZHANG Qing-xia, LIN Hai-ming, CAI Wei. Effect of nitrogen on growth and total alkaloids of Sophora flavescens[J]. Acta Prataculturae Sinica, 2009, 18(3): 159-164.

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